Microscopy

Question 1

Light Microscope Benefits

Answer 1

1,000 - 2,000 magnification
200nm resolution
easy and quick to use
low cost
living speciesism can be analysed

Question 2

Eye piece lens

Answer 2

Used to look through and has its own magnification

Question 3

Objective lens ,

Answer 3

Range of magnification lenses

Question 4

Condenser

Answer 4

Focuses the light from the light source onto the sample

Question 5

Iris

Answer 5

Focuses the size of the cone of line

Question 6

equation to calibrate a microscope

Answer 6

stage micrometer division
————————————- x no. of stage micrometer divisions
eyepiece graticule divisions

Question 7

Refractive index of a vacuum

Answer 7

1.0

Question 8

Comparative microscope

Answer 8

analyses specimens side by side
joined by an optical bridge
eg. firearms , bullet cartridges

Question 9

Polarised light microscope

Answer 9

• Used to view fibres
• Splits a ray into two - ordinary and extraordinary

Question 10

Fluorescence microscope

Answer 10

• Can be used to view pollen

Question 11

Electron microscope

Answer 11

SEM - Scanning electron microscope 2nm
TEM - Transsmisson electron microscope 1nm
2,000,000 magnification
sample prep is SLOW and complicated
expensive
- sample can’t be living