Microscopy Flashcards
What are the positives of a light microscope? - There cheap, easy to use, portable and can view living organsims.
What must scientific drawings include? - 1) state the magnification. 2) include a title. 3) Use a sharpened pencil for drawings and labelling. 4) draw smooth and continuous lines. 5) don’t shade. 6) label lines should not cross over each other. 7) label lines should not have arrow heads. 8) label lines should be parallel to the top of the paper.
What is the magnification formula? - Magnification = Image size/ actual size.
Nanometre to Micrometre - Divide by 1000
How many micrometers in a millimetre? - Divide by 1000
What are the two types of electron microscopes? - transmission (TEM) and scanning (SEM)
What are the benefits of an electron microscope? - you have a much better resolving power but it does kill what you look at, it had a higher resolution and magnification.
What are the disadvantages of electron microscopes? - There expensive, large and require instalment they also only view dead specimens.
What images are produced from each kind of microscope (light, TEM, SEM,LSM) - Light - 2D coloured image. TEM - 2D, Black & white image. SEM - 3D, Black & white image. LSM - 3D, coloured (with fluorescent dye) image.
Light microscope magnification and resolution - Resolution: 200nm Magnification: X1500
Laser scanning microscope magnification and resolution - Resolution: 160nm Magnification: X1500
SEM magnification and resolution - Resolution: 20nm Magnification: X200,000
LSM magnification and resolution - Resolution: 0.1 nm Magnification: 2,000,000
The image size is given in - Millimetres
The actual size is given in - Micrometres
Millimetre -> Micrometre -.> Nanometre - x1000
How do you stain a sample in microcopy? - Place stain at edge of a sample
lower the cover slip at an angle
use blotting paper to remove excess stain
use more than one stain (to improve contrast)
There cheap, easy to use, portable and can view living organsims.
What is the purpose of staining?
It gives the image a higher contrast, making it easier to distinguish and identify components within a cell.
What must scientific drawings include?
1) state the magnification. 2) include a title. 3) Use a sharpened pencil for drawings and labelling. 4) draw smooth and continuous lines. 5) don’t shade. 6) label lines should not cross over each other. 7) label lines should not have arrow heads. 8) label lines should be parallel to the top of the paper.
