Microscopy

Question 1

What is the range of depth visible to the human eye?

Answer 1

50-100 micrometres

Question 2

What is the range of sizes of cells in the human body?

Answer 2

5-40 micrometres

Question 3

State the fractions of a metre of a millimetre, micrometre and nanometre

Answer 3

milli: 10^-3
micro: 10^-6
nano: 10^-9

1 millimetre is a 1000 micrometres and 1 micrometre is 1000 nanometres

Question 4

What structures of an animal cell are visible with a high-powered light microscope?

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Answer 4

• Golgi apparatus
• mitochondria
• cytoplasm
• cell surface membrane
• centriole
• nucleus

Question 5

What structures of a plant cell are visible with a high-powered light microscope?

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Answer 5

• Golgi apparatus
• cytoplasm
• mitochondria
• vacuole
• cell surface membrane
• cell wall
• nucleus

Question 6

Define magnification

Answer 6

The number of times larger the image of an object is from its real size.

Question 7

What is the formula for magnification?

Answer 7

Image size/ Actual size

Question 8

Define resolution

Answer 8

The ability to distinguish between two separate points- the amount of detail that can be seen.

Question 9

What is the resolution of a light and electron microscope?

Answer 9

Light: 200 nm
Electron: 0.5 nm