microscopy

Question 1

What are the 5 types of light microcopy?

Answer 1

bright field, phase contrast, differential interference, dark field, and fluorescence

Question 2

what is bright - field microscopy?

Answer 2

Bright field microscopy is the simplest type: in order to generate contrast, cell components are stained/ dyed to make them visible

Question 3

what is phase shift microscopy?

Answer 3

Two types: in phase and out of phase –> when light moves in phase, light intensity is high. when light moves out of phase, peaks and troughs are added but cancels out, generating a dim image

Question 4

what is differential interference microscopy?

Answer 4

Differential interference microscopy manipulates phase differences to visualize edges or boundaries

Question 5

what is dark-field microscopy?

Answer 5

the only light that goes to our eye are the ones being scattered by the cell. You cannot see the outside of the cell as the image appears bright against a dark background

Question 6

What is immunocytochemisrty?

Answer 6

a lab technique to indicate where a certain protein is using the body’s own antibodies

Question 7

What is transmission Electron Microscopy?

Answer 7

In TEM, electrons shoot out of the electron gun at high velocity, focusing on the specimen, generating the image (higher electrons, shorter wavelength). Images that use TEM typically has a high resolution to see smaller and specific parts of the subject

Question 8

What is scanning electron microscopy?

Answer 8

SEM uses heavy metals on the specimen and scattered electrons bouncing off the it to generate an image. SEM produces a textured, 3D image but uses lower resolution and sees only external features