Microscopy Flashcards
Microscope Magnification
the number of time s the image of the specimen is made larger.
field of view
maximum area you can see through the ocular lens
specimen size
the actual size of the specimen being viewed
drawing magnification
the number of times larger a drawing is drawn compared to specimen size
resolution
point at which two objects can be distinguished separate
compound microscope
light rays are passed through
internal structures
cells and organisms can be observed while still living
dissecting microscope
light rays are bounced off a specimen
external structures
can be observed still living
transmission electron microscope (TEM)
beams of electrons are passed through thin sections of a specimen
internal structures of very small organisms may be viewed
images are projected onto computer or television screen
max magnification: ~1000000
Limit of resolution : 0.0000007
Scanning Electron Microscope (SEM)
Beams of electrons are bounced of specimens surface
external structures of very small organisms may be viewed
images are projected onto computer or television screen
max magnification: ~1000000
Limit of resolution : 0.0000007
Base
Bottom of microscope
Supports the microscope
arm
supports the body tube, objective lens and ocular lens as well
body tube
Connects the ocular lens to the objective lenses
coarse adjustment focusing knob
moves the stage closer and further from the lenses. general focus
fine adjustment focusing knob
an adjustment that is used for higher level lens to fine focus the image. Detail focus
low power objective lens
magnifies image 4X
medium power objective lens
magnifies image 10X
high power objective lens
magnifies image 40X
oil immersion objective lens
magnifies image 100X
ocular lens
lens at the top that you look through magnifies 10X
stage
the platform where the slides are placed
stage clips
holds the slides in place
revolving Nosepiece
holds objective lenses in place and revolves them
diaphragm
used to vary the intensity and the size of the cone of light that is projected upward into the slide
lamp
light source
condenser
focuses the light onto the specimen
Atom
smallest part of an element
Iron, magnesium
molecule
two or more atoms chemically combined
E.g. CO2, H2O
organic compounds
contain both carbon and hydrogen atoms
E.g. (DNA)
inorganic compound
lacks carbon and/or hydrogen atoms (CO2,H2O)
organelle
small structure within cells
E.g. chloroplast, mitochondria, Golgi apparatus
cell
basic unit of structure and functioning all living things
E.g. Fat cells cancer cells, skin cells
tissue
a group of specialized cells that perform a shared function
E.g. muscle tissue, blood, xylem
organ
a group of specialized tissues that work to perform a shared function
organ system
a group of interdependent organs, each with a specific function, that work together to carry out a major body function.
E.g. nervous reproductive, respiratory
multicellular organisms
an independently functioning living thing, compromised of multiple organ systems
population
a group of interacting and interbreeding organisms of one species.
community
a group of interacting populations in a specific regions
E.g. Mission creek park includes all the populations of bears, Kokanee salmon, trees, insects, etc
Ecosystem
the combination of all living and non living parts of the enviornment.
biome
major geographic areas in which the climate determines which life forms are present
biosphere
the entire world of living organisms
