Microscopic Methods Flashcards by NOE GEL ROY | Brainscape

Q

Illuminating source is the light.

A

Light Microscope

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Q

Specimen preparation takes usually few

minutes to hours.

A

Light Microscope

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Q

It has low resolving power (0.25µm to

0.3µm).

A

Light Microscope

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Q

It has a magnification of 500X to 1500X.

A

Light Microscope

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Q

The object is 5µm or thicker.

A

Light Microscope

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Q

Vacuum is not required.

A

Light Microscope

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Q

There is no need of high voltage electricity.

A

Light Microscope

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Q

There is no cooling system.

A

Light Microscope

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Q

Filament is not used.

A

Light Microscope

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Q

Radiation risk is absent.

A

Light Microscope

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Q

It is used for the study of detailed gross

internal structure

A

Light Microscope

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Q

Illuminating source is the beam of

electrons.

A

Electron Microscope

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Q

Specimen preparation takes usually takes

few days.

A

Electron Microscope

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Q

All lenses are electromagnetic

A

Electron Microscope

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Q

It has high resolving power (0.001µm),
about 250 times higher than light
microscope.

A

Electron Microscope

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Q

It has a magnification of 100,000X to

300,000X.

A

Electron Microscope

Q

The object is 0.1µm or thinner.

A

Electron Microscope

Q

Vacuum is essential for its operation.

A

Electron Microscope

Q

High voltage electric current is required

50,000 Volts and above

A

Electron Microscope

Q

It has a cooling system to take out heat

generated by high electric current

A

Electron Microscope

Q

Tungsten filament is used to produce

electrons.

A

Electron Microscope

Q

Image is received in zinc sulphate

fluorescent screen or photographic plate.

A

Electron Microscope

Q

It is used in the study of external surface,

structure of atom and very small particles.

A

Electron Microscope