Microscopic Examination

Question 1

Preferred specimen for micrscopic exam

Answer 1

Fresh specimen

Question 2

How much urine sample is required for microscopic exam?

Answer 2

10-15mL, 12mL avg

Question 3

RCF range and for how long

Answer 3

400-450, 5 mins

Question 4

RCF stands for ______

Answer 4

Relative centrifugal force

Question 5

Volume of the sediment to be examined, and the size of the cover slip

Answer 5

20uL or 0.02mL

22 x 22 mm

Question 6

Under LPO, how many fields are going to be examined?

Answer 6

10 fields

Question 7

Under HPO, how many fields are going to be examined?

Answer 7

10-20 fields

Question 8

Why is there a need to stain a urine sample?

Answer 8

It is done when there’s a need to target a specific sediment because the stain increases the overall visibility

Question 9

Sternheimer-Malbin stain is absorbed well by:

Answer 9

WBCs, epithelial cells, and casts

Question 10

Sternheimer-Malbin stain is composed of ______ and ______.

Answer 10

Crystal violet, Safranin O

Question 11

0.5% toluidine blue enhances the nuclear detail and differentiates….?

Answer 11

WBCs and RTEs

Question 12

Oil red O and Sudan III stains what?

Answer 12

Lipids (triglycerides, neutral fats, cholesterol)

Question 13

For the detection of eosinophil in drug induced allergic reaction, what stain is used?

Answer 13

Hansel stain

Question 14

If there is no Hansel stain available, what should you use instead?

Answer 14

Wright’s stain

Question 15

What stain helps in confirming the presence of hemosiderin granules in the urine?

Answer 15

Prussian blue stain

Question 16

How does hemosiderin form?

Answer 16

When an RBC gets lysed, it releases hemoglobin. When those hemoglobin reaches the nephron, it will be reabsorbed by the tubules, which turns it into hemosiderin

Question 17

RBC normal values in the urine

Answer 17

0-3/hpf

Question 18

RBCs may be confused with what?

Answer 18

Yeast cells, oil droplets, air bubbles, pus cells

Question 19

When correlating for other test for RBC presence, hemoglobin needs to exceed _____ for it to test positive

Answer 19

10 mg/dL

Question 20

Normal value for WBC in the urine

Answer 20

0-8/hpf

Question 21

In a hypotonic solution, what are WBC called?

Answer 21

Glitter cells

Question 22

WBCs may look like…?

Answer 22

Dead Trichomonads
Crenated RBCs
RTEs