Microscopes And Magnification

Question 1

What is a light microscopes use?

3 Advantages and 2 disadvantages

Answer 1

Uses light rays to observe objects
Advantages : can observe living specimen, cheap and portable, easy to set up and use, does not use harsh chemicals
Disadvantages : low magnification (x 2000 = max), low resolution

Question 2

Transmission EM uses

3 Advantages and 2 Disadvantages

Answer 2

Uses focused electron beams to see through sections of tissue
Advantages : high magnification ( max = 5 mil ), high resolution and can see details inside of cell
Disadvantages: Expensive, harsh chemicals used in preparation which can cause artefacts, dead specimen must be used

Question 3

Scanning EM uses

3 Advantages and 2 Disadvantages

Answer 3

Uses focused electron beams reflected off tissues
Advantages: high magnification ( max =5 mil ), high resolution, can see details of surfaces of structures
Disadvantages: can not view live specimen, harsh chemicals used in preparation can cause artefact, expensive

Question 4

Laser scanning confocal microscope uses

3 Advantages and 2 Disadvantages

Answer 4

Uses laser beam of light to illuminate chemical stains within specimen
Advantages: can view living cells, can observe cell processes by tracking molecules, higher resolution than light microscopes
Disadvantages: more complex and more expensive than light microscopes