Microscopes

Question 1

How will you protect the microscope?

Answer 1

• ALWAYS with 2 hands:
1 on Arm | 1 under Base
• COVER with plastic to prevent dust.
• Keep SWITCHED OFF and on LOW power objective.
• CLEAN lenses only with special cloth/lens cleaners. DO NOT USE TISSUE!
• KEEP away from water.

Question 2

Function of Body Tube

Answer 2

Separates the objectives and the eye piece. Align the light to the ocular lens.

Question 3

Function of Rotating Nose Piece

Answer 3

Holds the low to high power objectives and can be rotated to change magnification.

Question 4

Function of Low Power Objective

Answer 4

Magnifies at 4X.

Question 5

Function of Medium Power Objective

Answer 5

Magnifies at 10X.

Question 6

Function of High Power Objective

Answer 6

Magnifies at 40X.

Question 7

Function of Stage Clips

Answer 7

Holds the slide in place.

Question 8

Function of Diaphragm

Answer 8

Regulates the amount of light intensity on the specimen.

Specimen- on the slide.

Question 9

Function of Light Source

Answer 9

Illuminated the specimen by shine bright light through it.

Question 10

Function of Ocular Lens (inside eyepiece)

Answer 10

Magnifies10 times.

Question 11

Function of Arm

Answer 11

Support microscope when carried. It also supports the body tube and the objectives.

Question 12

Function of Stage

Answer 12

Supports the slide being viewed. Allows light to pass through at the opening.

Question 13

Function of Coarse Adjustment Knob.

Answer 13

Moves the stage up and down for focusing.

Question 14

Function of Fine Adjustment Knob.

Answer 14

Moves the stage slightly to sharpen the image.

Question 15

Function of Base

Answer 15

Supports the microscope and contains the electronics.

Question 16

How do I make a wet mount slide?

Mention ALL 6 points.

Answer 16

1) Get a cover slip from a teacher.
2) Place 1 drop of water in the middle of the slide. Don’t use too much or the water will run off the edge and make a mess!
3) Place a thin piece of the specimen on the slide.
4) Place 1 dip of iodine solution(plant cells) or bromothymol blue(animal cells) on the specimen to stain the cells.
5) Slowly lower the cover slip on top of the drop (45° angle)
6) Place the slide on the stage and view it first with the 4X Objective. Once you see the image, you can rotate the nosepiece to view the slide with the different objectives.