Microscopes Flashcards
Radiation used//electron microscope
Electron beam
Focusing with//light microscope
Lenses
Focusing with//electron microscope
Magnets
Magnification//light microscope
X1500
Magnification//electron microscope
X500,000
Resolving power//light microscope
200nm
Resolving power//electron microscope
0.2nm
Biological matter//light microscope
Living or dead
Biological matter//electron microscope
Dead
Size//light microscope
Small and portable
Size//electron microscope
Very large and static
Ease of use//light microscope
Easy to use
Ease of use//electron microscope
Skill and training required
Cost//light microscope
Relatively cheap
Cost//electron microscope
Very expensive
Sample in a vacuum//light microscope
No
Samples in a vacuum//electron microscope
Yes
Radiation used//Light microscope
Light rays
Transmission Electron Microscope (TEM)
Send electrons through a specimen and the image produced is 2D
Scanning Electron Microscope (SEM)
Bounce electrons off the surface of an object and the images are 3D
Why have electron microscopes
1) better magnification
2) better resolution
Magnification
Magnification=image/actual
