microscopes Flashcards

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1
Q

what is an eyepiece lens

A

the lens you look down

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2
Q

what is an objective lens

A

closest to the sample

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3
Q

what is magnification

A

the number of times greater that an image is than the actual object

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4
Q

what is resolution

A

the ability of a microscope to show two objects as separate

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5
Q

how do you calculate magnification

A

magnification = image size / actual size

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6
Q

what source does a light microscope use

A

light source

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7
Q

what source does a SEM and TEM use

A

electron source

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8
Q

how many micrometres are in a millimeter

A

1mm = 1000 micro metres

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9
Q

which microscope has the lowest resolution

A

light microscopes

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10
Q

what are light microscopes useful for

A

studying whole cells or tissues

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11
Q

what is the maximum magnification of a light microscope

A

around x1500

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12
Q

what do confocal microscopes use as a source

A

laser beams

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13
Q

what image is produced from a confocal microscope

A

3D

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14
Q

when do you use a confocal microscope

A

look at objects at different depths in thick specimens

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15
Q

what is good about electron microscopes

A

high resolution and high magnification

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16
Q

what image does a SEM produce

A

3D image

17
Q

what do you use TEM for

A

ultrastructure of cells