microscopes

Question 1

Advantage of a light microscope

Answer 1

can observe living cells for example mitosis

Question 2

What are the 2 disadvantages of a light microscope?

Answer 2

Stain will kill living cells
Low resolution (meaning cant see all the living organelles in the cell)

Question 3

Magnification and resolution

Answer 3

x1500, 200nm

Question 4

Why cant a light microscope have a high resolution?

Answer 4

Because it has a short wavelength of visible light (400nm - 700nm)

Question 5

Does it pass through the specimen?

Answer 5

Yes

Question 6

What is a electron microscope?

Answer 6

Uses a beam of electrons and needs to be in a vacuum

Question 7

What are the two types of electron microscopes?

Answer 7

SEM (Scanning electron microscope) and TEN(Transmission electron microscope)

Question 8

Why cant a electron microscope be used on living cells?

Answer 8

Because they would die in vacuum as there is no particles in there

Question 9

Do electron microscopes pass through the specimen?

Answer 9

Yes some parts of the specimen more easily than others

Question 10

Advantages of electron microscope (2)

Answer 10

High resolution
Have 2000x than light microscope

Question 11

Disadvantage of electron microscope

Answer 11

Uses a vacuum so cany us e living cells and require very careful staining and the specimen has to very thin

Question 12

Define resolution

Answer 12

The minimum distance between 2 objects where they can be seen as two separate objects

Question 13

Describe TEN

Answer 13

Uses beam of electrons
Electrons pass through the specimen and has to be very thin
Ultra structures are seen
Black and white images produced
Higher resolution
Magnification 100,00x - 500,000x
Resolution 0.1nm

Question 14

Describe SEM

Answer 14

Uses beam of electrons
Electrons pass back and forth across the specimen
Magnification=50,000x
Resolution 0.1nm

Question 15

Define staining

Answer 15

Provides a contrast between organelles and cytoplasm allowing structures to be observed