Microscope Quiz 2 Flashcards
What is the name of the oil used on the slide?
Immersion oil
What does each analyst have to do annually?
They have to do the ruler under microscope, with the single strip factor in the state book
True or False: Slides can be washed and reused.
True
What is the SSF if the diameter is .185?
Calculation: 10,000 / (11.28 x 0.185) = 10,000 / 2.0868 = 4,790
How do you agitate the sample?
25 times in less than 7 seconds
What is the limit for somatic cell count?
1 million
How often are thermometers calibrated?
Annually
Explain the steps after the slide comes out of the dryer box up to reading it under the microscope.
- Grip the slide with forceps
- Place in Levowitz-Weber Modification of the Newman-Lampert Canadian Stain for 2 minutes under a vented fume hood
- Remove from stain and place in front of a fan to dry
- Dip the slide 3 times in water at 35-45°C
- Let dry under the vented fume hood until dry
What is the temperature range for the water used to dip the slide?
35-45°C
How many times is the slide dipped in water?
3 times
List 5 errors that could go wrong with the dryer box.
- temp of box too low
- temp of box too high
- dirty dryer box
- dryer box not level
- lid of dryer box doesn’t close all the way
These errors can affect the performance and efficiency of the dryer box.
Give 3 reasons why you could possibly have a bad slide.
- dirty slide
- dirty pipette tip
- dirty dissecting needle
Cleanliness is crucial for accurate results in slide preparation.
Explain the process on how to make a slide starting from getting the milk sample.
- Agitate the sample 25 times in a 1 ft arc within 7 seconds
- Tap vial to dissipate the foam
- Draw up .01 mL of milk sample avoiding foam while holding vertical
- Wipe off excess milk on the outside of the tip away from the tip
- Dispense the milk on the center of the slide with one touch off
- Swirl the milk around the circle with a dissecting needle
- Close the lid and wait up to 5 minutes for it to be dry
This process ensures proper preparation for microscopic examination.
How do you round the following number to 2 sig figs? 145,000
140,000
Rounding rules apply to significant figures, focusing on the first two non-zero digits.
You can mix the milk sample on the slide with the dissecting needle like a hockey stick. True or False?
False
The correct technique is to swirl the milk, not mix it like a hockey stick.
What is the 2 sig figs of the following number? 155,000
160,000
Rounding up occurs because the third significant figure is 5.
