Microscope Quiz 1 Flashcards

1
Q

What is the ft/minute fume hood have to set at minimally?

A

100 ft/minute

The minimum airflow for fume hoods is crucial for safety in laboratory environments.

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2
Q

How often does the fume hood have to be checked?

A

Once a year

Regular checks ensure the fume hood is functioning properly to protect users from hazardous fumes.

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3
Q

What is the stain used for slides?

A

Levowitz Weber modification of the Newman lampert stain Canadian

The correct amount of stain is important for proper visualization of specimens.

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4
Q

What is the measurement of the slides have to be?

A

11.28 mm delineated

Accurate slide measurements are important for consistent results in microscopy.

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5
Q

Does the pipettor have to have a serial # and date calibrated on it?

A

Yes

Calibration and serial numbers are essential for traceability and ensuring accuracy in measurements.

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6
Q

How often is the pipettor calibrated?

A

6 months

Regular calibration maintains the accuracy and reliability of pipetting equipment.

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7
Q

What is the temperature of the dryer box?

A

40-45°C

Maintaining the correct temperature is vital for the drying process in slide preparation.

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8
Q

What is the name of the instrument that you swirl the milk on the slide with?

A

Dissecting needle

Dissecting needles are commonly used in microscopy to manipulate samples on slides.

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9
Q

How much milk do you draw up to put on slide

A

0.01 mL

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10
Q

What is mastitis characterized by?

A

Long chains of bacteria

Mastitis bacteria appear in long chains, while diplococci are seen in pairs.

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11
Q

What is the cut off limit of bacteria count for GLC?

A

300k

The options for the cut off limit include 200k, 300k, 150k, and 250k.

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12
Q

What is a somatic cell?

A

Large circular cell with nucleus that stains dark blue

Somatic cells are typically identified by their size and staining characteristics.

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13
Q

What does the abbreviation DMSCC stand for?

A

Direct Microscopic Somatic Cell Count

This term is used in assessing somatic cell levels in milk.

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14
Q

How long should the slide sit in the stain?

A

2 minutes

The options for staining duration include 1, 2, 3, and 4 minutes.

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15
Q

What should you do after shaking the milk sample for testing?

A

Allow to stand until foam disperses and use within 3 minutes

This step is crucial to ensure accurate sampling and testing.

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