Microscope Quiz 1 Flashcards
What is the ft/minute fume hood have to set at minimally?
100 ft/minute
The minimum airflow for fume hoods is crucial for safety in laboratory environments.
How often does the fume hood have to be checked?
Once a year
Regular checks ensure the fume hood is functioning properly to protect users from hazardous fumes.
What is the stain used for slides?
Levowitz Weber modification of the Newman lampert stain Canadian
The correct amount of stain is important for proper visualization of specimens.
What is the measurement of the slides have to be?
11.28 mm delineated
Accurate slide measurements are important for consistent results in microscopy.
Does the pipettor have to have a serial # and date calibrated on it?
Yes
Calibration and serial numbers are essential for traceability and ensuring accuracy in measurements.
How often is the pipettor calibrated?
6 months
Regular calibration maintains the accuracy and reliability of pipetting equipment.
What is the temperature of the dryer box?
40-45°C
Maintaining the correct temperature is vital for the drying process in slide preparation.
What is the name of the instrument that you swirl the milk on the slide with?
Dissecting needle
Dissecting needles are commonly used in microscopy to manipulate samples on slides.
How much milk do you draw up to put on slide
0.01 mL
What is mastitis characterized by?
Long chains of bacteria
Mastitis bacteria appear in long chains, while diplococci are seen in pairs.
What is the cut off limit of bacteria count for GLC?
300k
The options for the cut off limit include 200k, 300k, 150k, and 250k.
What is a somatic cell?
Large circular cell with nucleus that stains dark blue
Somatic cells are typically identified by their size and staining characteristics.
What does the abbreviation DMSCC stand for?
Direct Microscopic Somatic Cell Count
This term is used in assessing somatic cell levels in milk.
How long should the slide sit in the stain?
2 minutes
The options for staining duration include 1, 2, 3, and 4 minutes.
What should you do after shaking the milk sample for testing?
Allow to stand until foam disperses and use within 3 minutes
This step is crucial to ensure accurate sampling and testing.