Microscope Measurments And Caluculations

Question 1

How do you measure the size of an object when using a light microscope

Answer 1

Using an eyepiece graticule and a stage micrometer

Question 2

How do you calibrate the graticule

Answer 2

Line up and the scale on the eyepiece with that on the micrometer using an objective lens that magnifies 400 times. If 50 graticule units are equivalent to 10 micrometer units, if each micrometer unit is 10 then each graticule unit is 2 micrometers

Question 3

If the graticule equals 2 micrometers and they use an objective lens magnifying 100 times, what would each graticule unit be equivalent to

Answer 3

8 micrometers

Question 4

What is 10 units on the micrometer scale equal to in the graticule scale

Answer 4

40 units