Microscope:lab Flashcards
T/f The microscope revolutionized the way zoologist study, identify, classify, an describe small members of the kingdom animalia
t
T/f Microscope doesn’t enable research entities
False
T/f simple microscope is similar to the magnifying glass for it is composed of one lens only
True
What kind of microscope that is similar to magnifyingglass that has one lens only?
Similar microscope
What kind of microscope that has 2 lenses at the opposite ends of tube?
Compound microscope
Type of a lens that is close to the eye?
ocular lens
Type of a lens that is near the specimen
objective lens
T/F: the enlarged and magnified views of microscopic species allows you to examine their minute structures
T
Most microorganisms studied under a microscope are measured in? which is?
Micrometer: 1/1000 millimeter
Is useful when viewing a whole specimen of small organisms or separate body parts
dissecting microscope
What kind of microscope is used if the highly recommended magnification desired is 5x to 50x?
dissecting microscope
what kind of microscope that has 2 ocular lens
Binocular microscope
It is the space you see when you look into a microscope?
field of view
A light source in the microscope can be directed two ways: What are those?
downward onto the object and up through the object.
T/f by enabling the transmission light, you can visualize objects that are thin?
False
T/f you can zoom in or out an object if your microscope has knobs on its side
true
what is the proper way to carry dissecting microscope?
You should carry dissecting microscope by its arm using one hand and support the base by your other hand.
What are 6 parts of dissecting microscope?
light switch
illumination control knob
oculars
zoom knob
stage
focus knob
what turns on the illuminator?
light switch
what function that rotates to enable reflection or transmission of light?
illumination control knob
refers to the light that travels in an upward direction to the specimen?
transmission
refers to the light directed on a downward direction to the specimen?
reflection
T/f you can get the best illumination for your specimen if you rotate the knob when visualizing
t
move together or apart to allow adjustment according to your own level of comfort when looking into the microscope?
oculars
T/f the two fields of ocular should overlap completely so that you see a single circle of light
t
it allows you to control magnification and is found next to oculars?
zoom knob
is where you place your slide which contains the specimen you will be examining
stage
it can turn for sharpness of focus
focus knob
t/f specimens are placed directly on the stage of the microscopes?
False: fixed on the slide
where should the specimen are placed?
glass slide
what are the materials in dissecting ampalaya seed?
dissecting microscope
ampalaya seed
glass slide
What are the procedures in dissecting ampalaya?
1.) put ampalaya seed on the slide and focus under the dissecting microscope
2.) switch on the light to illuminate the specimen and rotate illuminating knob while looking through the oculars.
3.) move the zoom knob to control magnification.
4. draw the seed seen under the the dissecting microscope.
What are the parts of a compound microscope?
Mechanical
- arm, base, stage, stage clips, pillar, nosepiece, body tube, coarse adjustment knob,, light source, fine adjustment knob, iris diaphragm, draw tube
- Optical
-ocular lens or eye piece, objective lenses, condenser, iris diaphragm, mirror/bulb.
serves to support the body tube?
arm
provides firm and steady support to the entire microscope?
base
is where the slide is placed
stage
T/f It is important to grasp the microscope by the arm when transferring the microscope to another place.
t
how to hold a compound microscope?
keep it upright and the underneath using a free hand
what is the purpose of the hole in the stage?
so that light can shine up through the specimen
it holds the specimen firmly on stage
stage clips
is the support or post of the base where the arm is attached
pillar
is the revolving part to which the objectives are attached?
nosepiece
T/f it is necessary to firmly click (nosepiece) it into position when the objective is changed?
t
connects the nosepiece to the ocular lens
body tube
moves the body tube or stage in an upward or downward direction?
coarse adjustment knob
coarse adjustment know is utilized only with?
lowpower or scanning objectives
moves either the body tube or stage in an upward or downward direction to correctly position that specimen so that it can be focused perfectly.
fine adjustment knob
is usually a small electric light beneath the stage ?
light source
it can be used to reflect light from another source into the microscope?
mirror
regulates how much light and lamp heat pass through the specimen
iris diaphragm
is the small cylinder attached to the upper part of the body tube that holds the ocular
draw tube
is a detachable cylinder located at the upper end of the tube?
ocular lens or eyepiece
what are the 4 objective lenses in a microscope?
scanner
low power objective
high power objective
oil immersion objective
what is the magnification for LPO, HPO, OIO, AND SCANNER?
SCANNER- 5-10X RED
LPO- 10-15X YELLOW
HPO- 40-60X BLUE
OIO- 1000X- WHITE
is a lens located at above the diaphragm and concentrates light before passing through specimen?
condenser
2 kinds of mirror or bulb? and explain
flat(natural source) and concave(artificial light)
is used to collect light and direct it to the object an reflect it to the eyes
mirror
is the preferred method of specimen preparation?
Wet mount
t/f wet mount can oly be used for a short duration since it dries out faster
t
what are the materials for preparing wet mount?
compound microscope
hay infusion (canal water)
glass slide
cover slip
lens paper
dropper
teasing needle
ruler
what is the procedure for wet mount?
prepare the glass slide, cover slip and teasing needle. second, put a dropr of hay infusion or canal water on the center of glass slide. next, gently cover it with a cover slip with ur thumb and your slide us now ready for viewing under microscope.
what are the materials in viewing the letter e?
compound microscope, prepared slide of the letter e
glass slide
cover slip
lens paper
what are materials in using oil immersion
prepared slides of bacteria
immersion oil
what do use to remove oil off the lens?
use lens paper with xylene
are 2 important concepts to comprehend when viewing tiny object?
mangnification and resolution
t/f: when an object is greatly magnified, its image can be fuzzy
t
is the measure of how clearly one can visualize details under microscope
resolution
what is the total magnification?
ocular lens multiplied by the magnification of objective lens
the magnification of the object lens can be?
4, 10, 40, 100x
is used in linear measurement of microscopic specimen
micrometer eyepeice