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Histo Lab > Microscope and Cell > Flashcards

Microscope and Cell Flashcards

1
Q

Magnify image and allow visualization of greater details than is possible with unaided eye.

A

Light Microscope

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2
Q

2 Types of Microscope

A

Simple - One lens
Compound - Multiple lens

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3
Q

What type of microscope is magnifying glass?

A

Simple Microscope

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4
Q

What type of microscope is eyeglasss?

A

Simple Micrscope

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5
Q

What type of microscope is one ocular lens microscope?

A

Compound Microscope

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6
Q

What type of microscope is two ocular lens microscope?

A

Compound Microscope

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7
Q

Parts of Microscope

A

Ocular Lens
Objective Lens
Arm
Mechanical Stage
Stage Clip
Aperture
Condenser
Iris Diaphragm
Coarse Adjustment Knob
Fine Adjustment Knob
Light Source
Brightness Switch
Power Switch
Base

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8
Q

Proper Handling of Microscope

A

Cord must be secured
Support base with one hand
Lift with the arm of microscope

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9
Q

The power of microscope to enlarge the image of an object

A

Magnification

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10
Q

Ability of the microscope to distinguish two objcts from each other

A

Resolution

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11
Q

What property of microscope?

10x - 40x - 100x

A

Magnification

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12
Q

What property of microscope?

Blur to clear

A

Resolution

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13
Q

The compound microscope has two major parts

A

Optical part
Mechanical Part

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14
Q

Magnifies tissue specimens

A

Optical Parts

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15
Q

Supports the optical parts

A

Mechanical Parts

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16
Q

What are the Parts of the Optical Part

A

Ocular or Eyepiece
Objective Lens

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17
Q

Ocular or

A

Eyepiece

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18
Q

Lens that one looks into

A

Ocular lens or Eyepiece

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19
Q

Lens that is closest to the tissue or sample slide

A

Objective lens

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20
Q

Give the Magnification Power of the Objectives

A

Scanner 4x
Low Power 10x
High Power 40x
Oil Immersion Objective 100x

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21
Q

Formula of Total Power

A

Eyepiece Magnification x Objective Magnification

10 x 40(Low Power) = 400x

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22
Q

Holds the different objective Lenses

A

Revolving Nosepiece

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23
Q

It allows the rotation of the lenses while viewing

A

Revolving Nosepiece

24
Q

Supports the lenses and nosepiece of the microscope and connects the stage to the base of the clip

A

Arm

25
Q

Platform that is flat used for placing the slides under observation

A

Stag and Stage Clip

26
Q

Helps in controlling the amount of light that is passing through the opening of the stage

A

Iris Diaphragm

27
Q

Gathers light from the microscope’s light source and concentrate it into a con of light that illuminates the specimen

A

Condenser

28
Q

Lowers or Raise the stage

A

Coarse Adjustment Knob

29
Q

Used for focus on scanning. Usually the lower power lens is used enabling the movement of the tube.

A

Coarse Adjustment Knob

30
Q

Used for focusing the high power and oil objectives

A

Fine Adjustment Knob

31
Q

Moves the body tube for focusing the high power lens

A

Fine Adjustment Knob

32
Q

Source of Light

A

Light Source or Illuminator

33
Q

Moves the stage sideways

A

Stage control

34
Q

Steps in Focusing Specimens

A
  1. Always start with the Scanning Objective
  2. Place specimen in the center of your field of view.
  3. Use Coarse Adjustment Knob to focus
  4. Use the Fine Adjustment knob until clear
  5. Once focused on scanning, switch t o ow power obj using the revolving nosepiece
  6. Repeat steps 3-4
  7. Switch to High Power
  8. Only Use the fine adjustment knob to focus specimns
35
Q

Characteristics of Prokaryotes

A
  • Unicellular
  • No membrane bound nucleus
  • DNA is found in the nucleoid region
  • Absence of membrane bound organelles
36
Q

Characteristics of Eukaryotes

A

-Mostly are multicellular
-Some are unicellular
-With membrane-bound nucleus
- DNA is found within th nucleus
- Presence of Membrane bound organelles

37
Q

Yeast vs Mold

A

Yeast is multicellular
Mold is unicellular

38
Q

Difference between Prokaryotic DNA and Eukaryotic DNA

A

Prokaryotic DNA is short and circular while Eukaryotic DNA is longer and is contained in chromosomes

39
Q

T or F
Eukaryote is non-looped chromosomes

A

True

40
Q

What is the characteristics of Prokaryotic DNA

A

it is single looped

41
Q

Cell Wall of

Prokaryotes :
Plant Cell:
Fungal Cell:

A

Peptidoglycan
Cellulose
Chitin

42
Q

Eukaryotes undergoes cell division called

A

Mitosis

43
Q

Process of Mitosis

A

PMAT

Prophase
Metaphase
Anaphase
Telophase

44
Q

Major parts of Cell

A

Cell Membrane
Cytoplasm
Nucleus
Nucleolus
Chromatin

45
Q

How to study cells in histology?

A

Size
Shape
Nucleus
Staining Reaction

46
Q

What are the shapes of cell

A

Round/Spherical Cell
Cuboidal
Columnar
Pyramidal
Stellate
Spindle

47
Q

Cell Nucleus: Shape

A

Kidney bean Shaped - Monocyte

Lobed or Lobulated or Segmented - Neutrophil

Anucleated - No nucleus

48
Q

Kidney shaped nucleus

A

Monocyte

49
Q

Lobed shaped nucleus

A

Neutrophils

50
Q

Location of Nucleus

A

Central
Eccentric

51
Q

Hematoxylin is what color

A

Blue

52
Q

Hematoxylin is acidic or basic?

A

Basic

53
Q

Eosin is what color?

A

Red

54
Q

Eosin is acidic or basic?

A

Acidic

55
Q

Rule of Staining Reaction

A

Opposite attracts. Acids will attract Hematoxylin (basic) to achieve neutralization

Histo Lab (1 decks)

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