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Bacteriology Lab > Microscope > Flashcards

Microscope Flashcards

1
Q

Lab Safety Regulations

A
  • Put aside all unnecessary things
  • Conduct yourself in a responsible manner
  • Follow all written and verbal instructions
  • Wear PPE; Remove after
  • Wash hands before and after
  • Wear face shields or masks and eye protection
  • Treat all specimen as biohazard
  • Tie back hair
  • Decontaminate work area before and after
  • Do not eat, drink or smoke
  • Do not place anything in your mouth
  • Minimize creation of aerosols
  • Place all infectious wastes and materials in appropriate receptacle
  • Needles should not be bent, cut or recapped.
  • When infectious fluid is spilt, clean with disinfectant and notify instructor
  • Do not use cellphone during lab
  • familiarize all safety features in the lab
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2
Q

the study of organisms that are so small they cannot be seen with the naked eye.

A

Microbiology

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3
Q

minute organisms that cannot be seen by naked eye

A

microorganisms or microbes

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4
Q

Branches of Microbiology

A 
Bacteriology
Virology
Mycology
Parasitology
Phycology
Immunology
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5
Q

study of baceria

A

Bacteriology

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6
Q

study of viruses

A

Virology

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7
Q

study of fungi

A

Mycology

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8
Q

study of protozoa and parasitic worms

A

Parasitology

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9
Q

study of algae

A

Phycology

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10
Q

study of immune response

A

Immunology

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11
Q

Categories of Microbes

A

Cellular

Acellular

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12
Q

classifications of cellular microorganisms

A

Prokaryotes

Eukaryotes

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13
Q

microbes without true nucleus

A

Prokaryotes

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14
Q

microbes with true nucleus

A

Eukaryotes

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15
Q

classification of Prokaryotes

A

Eubacteria
Cyanobacteria
Archeans

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16
Q

true bacteria

A

Eubacteria

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17
Q

blue green algae

A

cyanobacteria

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18
Q

organisms that can survive in extreme environment

A

Archeans

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19
Q

classification of eukaryotes

A 
fungi
protozoa
algae
plants
animals
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20
Q

forms of acellular microbes

A

viruses

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21
Q

Why do we need to study bacteriology?

A
  • isolate and identify
  • pathogenic microorganisms
  • to assist
  • diagnosis, management and treatment
  • of infectious disease
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22
Q

is the entrance and multiplication of a microorganism in or on a host

A

infection

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23
Q

refers to an infection with functional and structura harm to the host that usually is accompanied by signs and symptoms

A

infectious disease

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24
Q

microorganism that is capable of causing infectious disease

A

pathogen

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25
Q
  • are microorganisms residing in a particular body site

- do not generally cause infection

A

normal flora or commensals

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26
Q
  • attacks on already debilitated host

- usually presents no danger to an individual with an intact immune system

A

opportunistic pathogen

27
Q

the science of investigating small objects using a microscope

A

Microscopy

28
Q

most common method used for both detection of microorganisms directly in the clinical specimens

  • and for the characterization of organisms grown in culture
A

microscopy

29
Q

defined as the use of microscope to magnify objects too small to be visualized with the naked eye so that characteristics are readily observable

A

microscopy

30
Q
  • the lens at the top that you look through

- usually 10x or 15x power

A

ocular or eyepiece

31
Q

used to magnify the object that is viewed from the eyepiece

A

objectives

32
Q

is the distance between the front lens of the objective and the specimen on the stage

A

working distance

33
Q

the higher the magnifying power,

A

the shorter the working distance

34
Q

where the specimen to be examined is placed

A

stage

35
Q

holds the specimen in place

A

stage clip

36
Q

holds two or more objective lenses and can be rotated to easily change power

A

revolving nosepiece

37
Q

other name of revolving nosepiece

A

turret

38
Q

produces light to illuminate and view the specime

A

light source/ illuminator

39
Q

gather light rays and focus these on the object to be examined

A

condenser

40
Q

is an opaque disk with an opening

can be expanded or contracted to increase or decrease the amount of light illuminating the object

A

iris diaphragm

41
Q

used to begin the focus

A

coarse adjustment knob

42
Q

used to finally adjust the details of a fuzzy focus

A

fine adjustment knob

43
Q

Types of Light Microscopes

A

Brightfield Microscope
Phase Contrast Microscope
Darkfield Microscope
Fluorescent Microscope

44
Q

Microscopes that can be used for bacteria

A

Brightfield
Fluorescent
Darkfield

45
Q

Microscopes that can’t be used for bacteria

A

Electron Microscopy

46
Q

Microscopes that can be used for fungi

A

Brightfield

Fluorescent

47
Q

Microscopes that can’t be used for fungi

A

Darkfield

Electron

48
Q

Microscopes that can be used for Parasites

A

Brightfield
Fluorescent
Electrone

49
Q

Microscopes that can’t be used for Parasites

A

Darkfield

50
Q

Microscopes that can be used for viruses

A

Fluorescent

Electron

51
Q

Microscopes that can’t be used for viruses

A

brightfield

darkfield

52
Q

Microscopes that are limitedly used for bacteria

A

Darkfield

53
Q

Microscopes that are limitedly used for parasites

A

Electron

54
Q

Microscopes that are limitedly used for viruses

A

Electron

55
Q

viruses are called ______ because they need a host in order to survive

A

obligate intracellular parasites

56
Q

other name of pathogenic

A

harmful microorganism

57
Q

common stomach commensal

A

lactobacillus acidophilus

58
Q

color of scanner

A

red

59
Q

color of LPO

A

yellow

60
Q

color of HPO

A

blue

61
Q

Color of OIO

A

white

62
Q

objectives used in coarse adjustment

A

scanner, LPO

63
Q

objectives used in fine adj.

A

HPO, OIO

Bacteriology Lab (2 decks)

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