Microscope Flashcards
Lab Safety Regulations
- Put aside all unnecessary things
- Conduct yourself in a responsible manner
- Follow all written and verbal instructions
- Wear PPE; Remove after
- Wash hands before and after
- Wear face shields or masks and eye protection
- Treat all specimen as biohazard
- Tie back hair
- Decontaminate work area before and after
- Do not eat, drink or smoke
- Do not place anything in your mouth
- Minimize creation of aerosols
- Place all infectious wastes and materials in appropriate receptacle
- Needles should not be bent, cut or recapped.
- When infectious fluid is spilt, clean with disinfectant and notify instructor
- Do not use cellphone during lab
- familiarize all safety features in the lab
the study of organisms that are so small they cannot be seen with the naked eye.
Microbiology
minute organisms that cannot be seen by naked eye
microorganisms or microbes
Branches of Microbiology
Bacteriology Virology Mycology Parasitology Phycology Immunology
study of baceria
Bacteriology
study of viruses
Virology
study of fungi
Mycology
study of protozoa and parasitic worms
Parasitology
study of algae
Phycology
study of immune response
Immunology
Categories of Microbes
Cellular
Acellular
classifications of cellular microorganisms
Prokaryotes
Eukaryotes
microbes without true nucleus
Prokaryotes
microbes with true nucleus
Eukaryotes
classification of Prokaryotes
Eubacteria
Cyanobacteria
Archeans
true bacteria
Eubacteria
blue green algae
cyanobacteria
organisms that can survive in extreme environment
Archeans
classification of eukaryotes
fungi protozoa algae plants animals
forms of acellular microbes
viruses
Why do we need to study bacteriology?
- isolate and identify
- pathogenic microorganisms
- to assist
- diagnosis, management and treatment
- of infectious disease
is the entrance and multiplication of a microorganism in or on a host
infection
refers to an infection with functional and structura harm to the host that usually is accompanied by signs and symptoms
infectious disease
microorganism that is capable of causing infectious disease
pathogen
- are microorganisms residing in a particular body site
- do not generally cause infection
normal flora or commensals
- attacks on already debilitated host
- usually presents no danger to an individual with an intact immune system
opportunistic pathogen
the science of investigating small objects using a microscope
Microscopy
most common method used for both detection of microorganisms directly in the clinical specimens
- and for the characterization of organisms grown in culture
microscopy
defined as the use of microscope to magnify objects too small to be visualized with the naked eye so that characteristics are readily observable
microscopy
- the lens at the top that you look through
- usually 10x or 15x power
ocular or eyepiece
used to magnify the object that is viewed from the eyepiece
objectives
is the distance between the front lens of the objective and the specimen on the stage
working distance
the higher the magnifying power,
the shorter the working distance
where the specimen to be examined is placed
stage
holds the specimen in place
stage clip
holds two or more objective lenses and can be rotated to easily change power
revolving nosepiece
other name of revolving nosepiece
turret
produces light to illuminate and view the specime
light source/ illuminator
gather light rays and focus these on the object to be examined
condenser
is an opaque disk with an opening
can be expanded or contracted to increase or decrease the amount of light illuminating the object
iris diaphragm
used to begin the focus
coarse adjustment knob
used to finally adjust the details of a fuzzy focus
fine adjustment knob
Types of Light Microscopes
Brightfield Microscope
Phase Contrast Microscope
Darkfield Microscope
Fluorescent Microscope
Microscopes that can be used for bacteria
Brightfield
Fluorescent
Darkfield
Microscopes that can’t be used for bacteria
Electron Microscopy
Microscopes that can be used for fungi
Brightfield
Fluorescent
Microscopes that can’t be used for fungi
Darkfield
Electron
Microscopes that can be used for Parasites
Brightfield
Fluorescent
Electrone
Microscopes that can’t be used for Parasites
Darkfield
Microscopes that can be used for viruses
Fluorescent
Electron
Microscopes that can’t be used for viruses
brightfield
darkfield
Microscopes that are limitedly used for bacteria
Darkfield
Microscopes that are limitedly used for parasites
Electron
Microscopes that are limitedly used for viruses
Electron
viruses are called ______ because they need a host in order to survive
obligate intracellular parasites
other name of pathogenic
harmful microorganism
common stomach commensal
lactobacillus acidophilus
color of scanner
red
color of LPO
yellow
color of HPO
blue
Color of OIO
white
objectives used in coarse adjustment
scanner, LPO
objectives used in fine adj.
HPO, OIO
