MICROSCOPE

Question 1

Used to magnify an object to a point where it can be seen by the human eye.

Answer 1

Magnifying glass

Question 2

Used to magnify an object to a point where it can be seen by the human eye.

Answer 2

Magnifying glass

Question 3

Is the science of investigating small objects and structures using such an instrument

Answer 3

Microscopy

Question 4

Invisible to the eye unless aided by a microscope

Answer 4

Microscopic

Question 5

Art of GRINDING LENSES is developed in Italy and SPECTACLES are made to improve eyesight

Answer 5

14th century

Question 6

The first to make microscope by placing two lenses in a tube

Answer 6

Dutch lens grinders Hans and Zacharias Janssen

Question 7

What year did Dutch lens grinders Hans and Zacharias Janssen make the first microscope

Answer 7

1590

Question 8

He studied various object with his microscope and publishes his results in MICROGRAPHIA. Among his work was description of cork and its ability to float in water.

Answer 8

Robert Hooke (1667)

Question 9

Uses a simple Microsoft with only one lens to look at blood, insects and many other objects. Was first to describe cells and bacteria, seen through his very small microscope with, for his time, extremely good lenses.

Answer 9

Anton van Leeuwenhoek (Father of Microscopy) 1675

Question 10

Several technical innovations make microscopes better and easier to handle, which leads to microscopy becoming more and more popular among scientists.

Answer 10

18th century

Question 11

An important discovery is that lenses
combining two types of glass could _____, with its disturbing halos resulting from

Answer 11

reduce the chromatic effect, differences in refraction of light

Question 12

He reduces the problem with spherical
aberration by showing that several
weak lenses used together at certain distances gave good magnification without blurring the image.

Answer 12

Joseph Jackson Lister (1830)

Question 13

This reduces the problem with spherical
aberration by showing:

Answer 13

several weak lenses used together

Question 14

three structural parts of the microscope

Answer 14

Head, Base, Arms

Question 15

it carries the optical parts in the upper part of the microscope

Basta dile na mao pag mag hunahuna kag letter E

Answer 15

Head

Question 16

it acts as the microscope’s support; it also carries the microscopic illuminators

Answer 16

Base

Question 17

the part that connects the base to the head and the eyepiece tube to the base of the microscope; it gives support to the head and used when carrying the microscope

Answer 17

Arms

Question 18

usually equipped with 10x lenses. The lenses
magnify the intermediate image formed by the objective lens in the optical
tube. They also limit the area of visibility

Answer 18

Eyepiece or oculars

Question 19

prevents scratching of lens by the user’s eyeglass or
vice versa

Answer 19

Eyepiece rubbers

Question 20

for differences in eye grading especially for
those wearing prescription glasses

Answer 20

Numbers on the eyepiece

Question 21

used to adjust the lateral separation of the oculars for each individual

(I P C)

Answer 21

Interpupillary control

Question 22

It provides a structural site of attachment for the revolving nosepiece.

Yes it can be two

Answer 22

Arm or neck

Question 23

holds the objectives and allows for easy rotation from one objective to another.

Answer 23

Revolving nosepiece

Question 24

Controls the height limit of the stage to prevent breakage of glass slide

Medj taas ni nga name

Answer 24

Knob/screw at the back of the microscope

Question 25

to tighten the screw

Answer 25

Clockwise

Question 26

to loosen the screw

Answer 26

Counterclockwise

Question 27

Also known as the **coarse tension adjustment knob**, **Prevents the stage** from **going down** and **losing focus** on the specimen |Yes medj taas napd siya nga name

Answer 27

Inner knob before the coarse adjustment knob

Question 28

It refers to the **thickness of the coverslip** to be used; coverslip to be used must be **compatible with the objective** to be used for microscopic examination - N o t O

Answer 28

Numbers on the objectives

Question 29

Most objectives have 3 to 4 lenses

Answer 29

Scanner Objective (4x) Low Power Objective (10x) High Power Objective (40x) Oil Immersion Objective (100x)

Question 30

a hole on the microscope stage through which the transmitted light from the source reaches the stage

Answer 30

Aperture

Question 31

supports the prepared microscope slide to be reviewed. Used for much easier to manipulate the object being observed | (yes not the base)

Answer 31

Mechanical stage

Question 32

It is also available by turning the **knob** left and right or backward and forward

Answer 32

Mechanical stage knob

Question 33

determines the **positioning** of the cells under the microscope; provides the **coordinates or location** of important cells "With numbers"

Answer 33

Stage numbers

Question 34

is also present to keep the slide stationary

Answer 34

Stage clip

Question 35

**focuses** or **directs** the **available light** into the objective as it *raised or lowered*. Lowering it will increase the contrast of unstained specimens. |Basta wala ni numbers

Answer 35

Condenser

Question 36

**must** be the **same with the magnification of the objective** for **better viewing of the specimen details** | Yes naay numbers ang last name

Answer 36

Condenser numbers

Question 37

regulates the **amount of light** that strikes the object being viewed. May be adjusted by a **movable lever**.

Answer 37

Iris Diaphragm

Question 38

It is used to focus with the **low power objective only**

Answer 38

Coarse adjustment knob

Question 39

gives a sharper image after the object is brought into view with coarse adjustment. Used in Higher Power Objective

Answer 39

Fine adjustment knob

Question 40

located within the base. For adjustment of the illumination for brightness | It is not the light source but a knob

Answer 40

Brightness adjustment knob/ Microscopic illuminator

Question 41

a firm, horseshoe-shaped foot on which the microscope rests’ it contains the light or mirror | (not a mirror pls)

Answer 41

Base

Question 42

the distance between the objective and the slide when objective is sharp focus.

Answer 42

Working distance

Question 43

if the magnification of the objective is higher?

Answer 43

the shorter the working distance

Question 44

should not be used when using higher magnifications

Answer 44

Coarse adjustment

Question 45

DIFFERENT TYPES OF MICROSCOPES | 3 types

Answer 45

COMPOUND/UPRIGHT INVERTED STEREOMICROSCOPE

Question 46

Objective lens faces downward, and specimens is observed from above. Suitable for observation of glass slides or prepared slides Used for routine microscopic examinations | (TYPE OF MICROSCOPE- SPECIFIC)

Answer 46

COMPOUND/UPRIGHT

Question 47

Objective lens faces upwards, and specimen is observed from below Used for observation of culture container (stem cells/unstained cells) | (TYPE OF MICROSCOPE) - SPECIFIC

Answer 47

INVERTED

Question 48

2 separate light path, enables observation of specimen from different angles at both eyes. Allow 3D observation of sample Suitable for animal works and large sample imaging | (TYPE OF MICROSCOPE)- SPECIFIC

Answer 48

STEREOMICROSCOPE

Question 49

APPLICATIONS OF THE MICROSCOPE | NOT THE TYPES BFM-DFM-PCM-EM(TEM,SEM)

Answer 49

BRIGHT FIELD MICROSCOPY DARK FIELD MICROSCOPY PHASE CONTRAST MICROSCOPY ELECTRON MICROSCOPY - Transmission Electron Microscope (TEM) - Scanning Electron Microscope (SEM)

Question 50

The **simplest** of all the optical **microscopy illumination techniques** Specimen’s image appears dark against a **bright background** Light source is usually **tungsten lamp** *Generally used* with *compound microscopes* | APPLICATIONS BUT SPECIFIC

Answer 50

BRIGHTFIELD MICROSCOPY

Question 51

Ability to **distinguish** between two **adjacent points** Depends on two factors: *White light & Numerical aperture* | R P

Answer 51

RESOLVING POWER

Question 52

Composed of a mixture of colored lights of various wavelengths

Answer 52

WHITE LIGHT

Question 53

Expression relating to the cone of light that is delivered to the specimen by the condenser and gathered by the objective | YES (w/ numbers again)

Answer 53

NUMERICAL APERTURE

Question 54

it is the ocular magnification multiplied by the objective magnification

Answer 54

TOTAL MAGNIFICATION

Question 55

Scanning Objective | the magnification/ocular lens/total magnification

Answer 55

4x 10x 40x

Question 56

Low power objective | the magnification/ocular lens/total magnification

Answer 56

10x 10x 100x

Question 57

High power objective | the magnification/ocular lens/total magnification

Answer 57

40x 10x 400x

Question 58

Oil immersion objective | the magnification/ocular lens/total magnification

Answer 58

100x 10x 1000x

Question 59

Uses a special optic system which converts differences in phases into differences in intensity such that some structures appear darker than the others Reveals the gross details of the internal structures in a living cell | APPLICATIONS BUT SPECIFIC

Answer 59

PHASE CONTRAST MICROSCOPY

Question 60

Objects appear brilliantly illuminated against a black background Used to demonstrate spirochetes which are difficult to observe in transmitted light | APPLICATIONS BUT SPECIFIC

Answer 60

DARKFIELD MICROSCOPY

Question 61

Develops an image resulting from variable electron density of the specimen interposed in the electron beam = Produces **monotone, two-dimensional, high magnified images** = **Resolving power** is 0.001 um, which is **200X** that of light microscopes = Specimens **must be fixed, stained and dried** = Used to observe **internal ultra-structural detail of cells** and **observations of viruses or small bacteria** | APPLICATIONS BUT SPECIFIC

Answer 61

TEM (TRANSMISSION ELECTRON MICROSCOPE)

Question 62

Has a practical limit of resolution of 0.005um or five-fold less than that of TEM = Produces monotone, tri-dimensional image by detection at a 90 degrees angle of secondary electrons emitted from the specimen surface as a result of bombardment by the primary electron beam = Used to observe the surface details of structures | APPLICATIONS BUT SPECIFIC

Answer 62

SEM (SCANNING ELECTRON MICROSCOPE)