MICROSCOPE Flashcards
1
Q
kinds of microscope
A
- Light Microscope
- Electron Microscope
2
Q
types of Light Microscope
A
- Compound Microscope
- Dissecting Microscope
3
Q
describe a Compound Microscope
A
- two-lens system (eyepiece and objective)
- magnification up to 1,500x
- specimen are sliced thinly
4
Q
describe a Dissecting Microscope
A
- AKA Stereo Microscope
- magnification up to 30x
- opaque specimen
5
Q
types of Electron Microscope
A
- Transmission Electron Microscope (TEM)
- Scanning Electron Microscope (SEM)
- Scanning Transmission Electron Microscope (STEM)
Scanning Tunneling Microscope (STM)
6
Q
parts of a Dissecting Microscope
A
- eyepiece
- diopter
- focus knob
- stereo head
- top lighting
- bottom lighting
- light controls
- stage plate
- stage clips
7
Q
describe an Electron Microscope
A
- use flow of electrons
- used to examine proteins, viruses, lipids, ribosomes and other small molecules
- has high resolution
- can magnify objects in nanometers
8
Q
describe a Fluorescence Microscope
A
- uses UV light to illuminate the specimen
- “epifluorescence microscopes” (reflect parallel beam of light directly upward to maximize illumination)
- used for life science (labelling highly specific structures within a cell) and biomedical research (analysis of biotechnology and immunotechnology)
- used to determine deoxyribose, DNA, antibodies, and antigens
9
Q
describe a Phase Contrast Microscope
A
- uses a special condenser
- enhance the contrast of the image of transparent specimens
10
Q
describe a Phase Contrast Microscope
A
- uses a special condenser
- enhance the contrast of the image of transparent specimens
11
Q
who is the Father of Microscopy?
A
Anton Van Leeuwenhoek
12
Q
who created the first ever microscope?
A
Hans & Zacharias Janssen
13
Q
who discovered the Cell Theory?
A
Robert Hooke
14
Q
what is used to clean the optical parts of a microscope?
A
lens paper
