MICROSCOPE Flashcards
early 1600s microscope are:
von leeuwanhoek simple microscope and janssens compound microscope
1670
hooks microscope
1850
powell and lealand no.1
1930
zeiss laboratory microscope
this microscope uses visible light to generate images
light (optical) microscope
what are the diff types of microscopes
- simple
- compound
- stereo
- digital
- fluorescence
- inverted
- automated imaging system
- confocal
- super resolution microscopy
- imaging flow cytometry
- magnification up to 300 times
- used to look at opaque objects
- provide close up 3d view of objects
- use in biological and medical science applications
Stereo (Dissecting) Microscope
- use laser light to scan samples
- with aid of dichromatic mirror
- produce maginified image on computer screen
confocal microscope
- uses electron rather than light
- sample are scanned in vacuum or near vacuum
- undergoing dehydration and then being coated
scanning electron microscope
- uses electrons creating magnified image
- offers high degree of both magnification and resolution
Transmission Electron Microscope
what part is used for adjustment and support?
Mechanical
whats part is used for enlarging the specimen?
magnifying parts
whats part is used for providing light?
illuminating parts
- Bottommost portion that supports entire/lower microscope
Base
part above the base supports other parts
pillar
allows tilting of microscope for convenience of user
inclination joint
curved/slanted part, held while carrying microscope
arm/neck
attached to arm and bears lenses
body tube
cylindrical structure on top of body tube holds ocular lenses
draw tube
platform where object be examined is placed
stage
secures specimen to stage
stage clips
allows light to pass through specimen for better view
aperture
also known as body, carries optical parts in upper part of microscope
head
when turned, raises or lowers eyepiece in order to adjust the focus for each eye
diopter adjustment
rotating disc where objective attached
revolving/ rotating nosepiece
lies atop nosepiece and keeps dust from settling on objectives
dust shield
geared to body tube which elevates or lowers when rotated bringing object into approx focus
coarse adjustment knob
smaller knob for delicate focusing bringing object into perfect focus
fine adjustment knob
elevates and lowers condenser
condenser adjustment knob
lever in front of condenser and which moved horizontally to open/close diaphragm
Iris diaphragm lever
gather wavefronts from microscopic light source and concentrate them into a cone of light
condenser
controls the amount of light reaching specimen
iris diaphragm
located beneath stage and has concave and plane surfaces to gather and direct light
mirror
built in illuminator beneath stage that may be used if sunlight is not prefered
electric lamp
another set of lens found on top of the body tube which function to further magnify the image produced by objective lenses
ocular/eyepiece
metal cylinders attached below nosepiece
objectives
objectives that gives lowest, 10x
Low power objective
objective gives higher, 40x
high power objective
objective gives highest magnification. 100x, used wet with cedar wood oil or synthetic oil
oil immersion objective
provide observes with enough, 4x
scanner
ability of lens to distinguish 2 objects that are close together
Resolution
function of objective lenses and its ability to gather light
numerical aperture
resolving power of compound microscope?
200 nanometer (nm)
resolving power of transmission electron microscope?
0.2 nanometer
resolving power of scanning electron microscope
10 nanometer
degree of enlargement
magnification
useful magnification and empty magnification
- useful; 500x-1000x
- empty: 1400x and beyond
refers to objectives that can be changed with minimal or no refocusing
parfocal
determined by linear measurement of objective front lens to specimen
working distance
- extent of observable area in distance units
- area you see under microscope
Field of view
used to measure minute distances or apparent diameters
micrometer microscope
