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MICRO PARA LAB > MICROSCOPE > Flashcards

MICROSCOPE Flashcards

1
Q

early 1600s microscope are:

A

von leeuwanhoek simple microscope and janssens compound microscope

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2
Q

1670

A

hooks microscope

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3
Q

1850

A

powell and lealand no.1

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4
Q

1930

A

zeiss laboratory microscope

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5
Q

this microscope uses visible light to generate images

A

light (optical) microscope

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6
Q

what are the diff types of microscopes

A
  • simple
  • compound
  • stereo
  • digital
  • fluorescence
  • inverted
  • automated imaging system
  • confocal
  • super resolution microscopy
  • imaging flow cytometry
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7
Q
  • magnification up to 300 times
  • used to look at opaque objects
  • provide close up 3d view of objects
  • use in biological and medical science applications
A

Stereo (Dissecting) Microscope

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8
Q
  • use laser light to scan samples
  • with aid of dichromatic mirror
  • produce maginified image on computer screen
A

confocal microscope

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9
Q
  • uses electron rather than light
  • sample are scanned in vacuum or near vacuum
  • undergoing dehydration and then being coated
A

scanning electron microscope

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10
Q
  • uses electrons creating magnified image
  • offers high degree of both magnification and resolution
A

Transmission Electron Microscope

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11
Q

what part is used for adjustment and support?

A

Mechanical

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12
Q

whats part is used for enlarging the specimen?

A

magnifying parts

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13
Q

whats part is used for providing light?

A

illuminating parts

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14
Q
  • Bottommost portion that supports entire/lower microscope
A

Base

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15
Q

part above the base supports other parts

A

pillar

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16
Q

allows tilting of microscope for convenience of user

A

inclination joint

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17
Q

curved/slanted part, held while carrying microscope

A

arm/neck

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18
Q

attached to arm and bears lenses

A

body tube

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19
Q

cylindrical structure on top of body tube holds ocular lenses

A

draw tube

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20
Q

platform where object be examined is placed

A

stage

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21
Q

secures specimen to stage

A

stage clips

22
Q

allows light to pass through specimen for better view

A

aperture

23
Q

also known as body, carries optical parts in upper part of microscope

A

head

24
Q

when turned, raises or lowers eyepiece in order to adjust the focus for each eye

A

diopter adjustment

25
Q

rotating disc where objective attached

A

revolving/ rotating nosepiece

26
Q

lies atop nosepiece and keeps dust from settling on objectives

A

dust shield

27
Q

geared to body tube which elevates or lowers when rotated bringing object into approx focus

A

coarse adjustment knob

28
Q

smaller knob for delicate focusing bringing object into perfect focus

A

fine adjustment knob

29
Q

elevates and lowers condenser

A

condenser adjustment knob

30
Q

lever in front of condenser and which moved horizontally to open/close diaphragm

A

Iris diaphragm lever

31
Q

gather wavefronts from microscopic light source and concentrate them into a cone of light

A

condenser

32
Q

controls the amount of light reaching specimen

A

iris diaphragm

33
Q

located beneath stage and has concave and plane surfaces to gather and direct light

A

mirror

34
Q

built in illuminator beneath stage that may be used if sunlight is not prefered

A

electric lamp

35
Q

another set of lens found on top of the body tube which function to further magnify the image produced by objective lenses

A

ocular/eyepiece

36
Q

metal cylinders attached below nosepiece

A

objectives

37
Q

objectives that gives lowest, 10x

A

Low power objective

38
Q

objective gives higher, 40x

A

high power objective

39
Q

objective gives highest magnification. 100x, used wet with cedar wood oil or synthetic oil

A

oil immersion objective

40
Q

provide observes with enough, 4x

A

scanner

41
Q

ability of lens to distinguish 2 objects that are close together

A

Resolution

42
Q

function of objective lenses and its ability to gather light

A

numerical aperture

43
Q

resolving power of compound microscope?

A

200 nanometer (nm)

44
Q
A
45
Q

resolving power of transmission electron microscope?

A

0.2 nanometer

46
Q

resolving power of scanning electron microscope

A

10 nanometer

47
Q

degree of enlargement

A

magnification

48
Q

useful magnification and empty magnification

A
  • useful; 500x-1000x
  • empty: 1400x and beyond
49
Q

refers to objectives that can be changed with minimal or no refocusing

A

parfocal

50
Q

determined by linear measurement of objective front lens to specimen

A

working distance

51
Q
  • extent of observable area in distance units
  • area you see under microscope
A

Field of view

52
Q

used to measure minute distances or apparent diameters

A

micrometer microscope

MICRO PARA LAB (3 decks)

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