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Anaphy Lab > MICROSCOPE > Flashcards

MICROSCOPE Flashcards

1
Q

are instruments that are used in science laboratories, to visualize very
minute objects such as cells, tissues, microorganisms, giving a contrasting image,
that is magnified.

A

Microscopes

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2
Q

􏰀 Microscopes are made up of lenses for —, each with their own
magnification powers. Depending on the type of lens, it will magnify the specimen according to its —.

A

magnification, focal strength

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3
Q

offer the ability to see great detail of objects that are hundreds of times too small to see with your naked eye.

A

Microscopes

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4
Q

PARTS AND FUNCTIONS OF A COMPOUND MICROSCOPE

A

Mechanical Parts
Optical Parts
Illuminating Parts

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5
Q

Mechanical Parts

A

Base
Pillar
Arm
Stage
Stage Clip
Draw tube
Revolving nosepiece
Coarse focus knob
Fine focus knob

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6
Q

Optical Parts

A

Ocular/Eyepiece
Objectives
- scanning obj
- low power obj
- high power obj
- oil immersion obj

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7
Q

Illuminating Parts

A

Illuminator/lamp
Mirror
Substage
- iris diaphragm
- condenser

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8
Q

is determined by multiplying the power of the ocular by the power of the objective in use.

A

Total magnification

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9
Q

Magnification:

Scanning
Low Power
High Power
Oil Immersion

A

4x
10x
40x
100x

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10
Q

Total Magnification

Scanning
Low Power
High Power
Oil Immersion

A

40x
100x
400x
1000x

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11
Q

Ocular Lens

A

10x

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12
Q

USING THE MICROSCOPE

A

Always carry with 2 hands
Never touch the lenses with your fingers.
• Only use lens paper for cleaning
• Do not force knobs
• Keep objects clear of desk and cords
• When you are finished with your “scope”, rotate the nosepiece so that it’s on the low power objective, roll the stage down to lowest level, rubber band the cord, then replace the dust cover.

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13
Q

Care and Maintenance of the Microscope

A

• Good preventive maintenance and care includes:
• Regular cleaning of oculars and objectives
• Avoid damaging oculars and other optics with eye make-up or other debris
• Careful handling to avoid abrupt motions
• Protect from direct sunlight, high temperature, humidity, dust and vibration
• Use appropriate materials to clean the lenses
• Cover when not in use with vinyl or plastic dust cover

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14
Q

USING THE MICROSCOPE

Focusing Specimens

A
  1. Always start with the scanning objective.

Once you’ve focused on Scanning, switch to Low Power.

  1. Now switch to High Power.
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15
Q

Using High Power

A

• Your slide MUST be focused on low power before attempting this step
• Click the nosepiece to the longest objective
• Do NOT use the Coarse Focusing Knob, this could crack the slide or the lens
• Use the Fine Focus Knob to bring the slide into focus

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16
Q

Scanning –> use
2. Low power -> use
3. High power –> use

A

coarse knob

coarse knob

fine knob

17
Q

at which the microscope stands on. It is a U-shaped structure.

A

Base (Foot):

18
Q

: It is the stand that lies on the stage and is a perpendicular projection.

A

Pillar

19
Q

: The whole microscope is managed or carried by the curve-shaped structure called the.

A

Arm

20
Q

: It is the rectangular structure that has a hole in the center that allows the light to pass through it. It is below the eyepiece, and most of the specimens are placed on this stage to examine.

A

Stage

21
Q

: It is the point where the pillar meets the arm. The whole microscope can tilt at this.

A

Inclination Joint

22
Q

: The stage, on the side of the specimen holder, contains clips that hold the slides on which samples will be placed.

A

Clips

23
Q

: It is a structure located below the stage which controls the intensity of light entering the stage. It is of two types: disc and iris.

A

Diaphragm

24
Q

: The body tube has three holes that have places to fit the objective lens. This structure is called the.

A

Nose piece

25
Q

: It is the hollow tube at the upper arm, which has one end of the eyepiece lens, and another term has an objective lens. It is altered using the adjustment knobs.

A

Body tube

26
Q

: It is a tiny knob that is used for focusing on the slide accurately. This knob can make only small adjustments.

A

Fine adjustment knob

27
Q

: It is a comparatively bigger knob that is used for more significant alterations.

A

Coarse adjustment knob

28
Q

: It is the lens used on the upper part of the body tube. It usually has a resolution of 10x but can be of 5x or 15x. It is the lens through which the person views the specimens.

A

Eyepiece

29
Q

: It is the lens used on the lower part of the body tube. It is attached to the nose piece. Usually, three or four nose pieces are available, which contain the objective lens of different resolutions like 10x, 40x, 100x.

A

Objective lens

30
Q

: A concave mirror on one side and plain mirror, on the other hand, can be seen below the stage to focus the light on the stage for a brighter image of the specimen.

A

Mirror

31
Q

It is seen below the stage and made up of a system of convex lenses which focus light from illuminating sources and is used to condense light towards the object.

Lowering the diminishes illumination whereas raising the increases the illumination.

A

Sub stage condenser:

32
Q

It is seen immediately below the condenser and operated by small lenses which protrude to one side.

Opening and closing of controls the light reaching the object.

A

Iris diaphragm:

33
Q

is situated at the base of the microscope. It is controlled by an ON /OFF switch and a lamp rheostat.

A

Light source:

34
Q

Odds are, you will be able to see something on this setting. Use the Coarse Knob to focus, image may be small at this magnification, but you won’t be able to find it on the higher powers without this first step.
Do not use stage clips, try moving the slide around until you find something.

A

Always start with the scanning objective.

35
Q

Use the Coarse Knob to refocus. Again, if you haven’t focused on this level, you will not be able to move to the next level.

A

Once you’ve focused on Scanning, switch to Low Power.

36
Q

If you have a thick slide, or a slide without a cover, do NOT use the high power objective. At this point, ONLY use the Fine Adjustment Knob to focus specimens.

A

Now switch to High Power.

37
Q

Your slide MUST be focused on low power before attempting this step
• Click the nosepiece to the longest objective
• Do NOT use the Coarse Focusing Knob, this could crack the slide or the lens
• Use the Fine Focus Knob to bring the slide into focus

A

Using High Power

Anaphy Lab (2 decks)

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