Microscope Flashcards
you only need a slight adjustment between objectives
Parfocal
bright background and dark object
Bright field
dark background and light object
Dark field
Holds the ocular lenses, and revolving nosepiece; also serves as a support for the microscope; the other hand hold this part when carrying the microscope
Arm
OIO - 100x - white HPO - 40x / 50x - blue LPO - 10x - yellow Scanner - 4x - red
Total Magnification = (objective) (ocular)
Objectives
Where objectives are attached
Revolving nosepiece
Serves as a support for the microscope; one hand is placed here when carrying a microscope
Base
Has a magnification of 10x
Objective lenses
Where the slides are places
Stage
Holds the slides in place
Stage clips
Found at the side of the microscope and are used for refining the image seen in the microscope
Course adjustment knob
Adjusts light passing through
Diaphragm
Adjusts light intensity
Rheostat
Concentrates light entering the lens
Condenser
Used for stained linearly oriented structures of living cells in tissue culture or fixed stained preparation.
Polarizing microscope
It restricts the probing light to
preferred directions and orientations making it possible to detect the
presence of orderly arranged fibrous proteins or other arrays of long molecules.
Polarizing microscope
Used for anisotropic substances — usually crystals (ex. uric acid, cholesterol)
Polarizing microscope
Used in measurement applications. They provide
Confocal microscope
A high-resolution image with all areas in focus throughout the field of view. Also enables non-contact non-destructive measurement of three dimensional shapes.
Confocal microscope
The image is more focused thus, small area is focused
Confocal microscope
Ultraviolet microscopes can image microscopic samples in the visible and the ultraviolet region.
Uv microscope
Allows the visualization of naturally fluorescent substances (fluorescent- antibody (FA) technique, or immunofluorescence) or those that have been stained with a fluorochrome to produce an image
Fluorescence microscope
Proides a 3D image showing very fine structural detial by splitting the ligh ray so that the beams pass through different areas of the specimen
Interference microscope
Used to enhance the contrast in unstained or transparent samples to visualize living cells or tissues
- Cell additives: protoplasmic
Interference microscope
- Note: Specimens should be transluscent, fixed and stained to provide contrast and to increase visualization
Objects appear dark against a light background illuminated by a condenser. It is where stained tissues are examined with ordinary light passing through the preparation
Image is inverted of the actual image
Bright field microscope
- Enhances visualization of elements with low refractive.
- Mineral elements, Spindle fibers
- Confocal Microscope
- The phase contrast microscope enables examination of unstained cells and tissues and is especially useful for living cells.
- Function
- Used in measurement applications. They provide
- A high-resolution image with all areas in focus throughout the field of view. Also enables non-contact non-destructive measurement of three dimensional shapes.
- A type of light microscopy that intensifies contrasts of transparent and colorless objects by influencing the optical path of light.
Phase contrast microscope
- Uses electron beams
- High resolution and magnification
- Subcellular structures: organelles
- In microbiology: visualization and study of viruses
Electron microscope
Used to enhance visualization of specimens that cannot be seen easily with a bright-field
microscope.
It can be effectively used at high magnifications to photograph living bacteria, or at low magnifications to view and photograph cells, tissues, and whole mounts.
Dark field microscope
