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PARASITOLOGY LAB > MICROSCOPE > Flashcards

MICROSCOPE Flashcards

1
Q

Houses the optical parts found in the upper
part of the microscope

A

HEAD

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2
Q

o Supports the microscope
o Houses the illuminator
o Must rest on a flat surface

A

BASE

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3
Q

o Connects the head and the base
o When carrying the microscope, we hold the
arm with one hand, and the other hand
supporting the base

A

ARM

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4
Q

o Another name for revolving nosepiece
o Holds the objective lens
o Used when switching objectives

A

Turrets

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5
Q

o Characterized as a square platform with a
hole/opening at the center

A

STAGE

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6
Q

opening at the center
o Where the slide with the specimen is
placed

A

APERTURE

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7
Q

holds the slides in place

A

STAGE CLIPS

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8
Q

allows you to move the stage
front or back

A

Y AXIS KNOBS

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9
Q

allows you to move the stage
left or right

A

X AXIS KNOBS

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10
Q

Used for the initial focusing of the
specimen

A

Coarse Adjustment knobs

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11
Q

Objective lens: Scanner/LPO

A

Coarse Adjustment knobs

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12
Q

Make the image more vivid

A

Fine adjustment knobs

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13
Q

Objective lens: HPO/OIO
§ Do not use when using
Scanner/LPO

A

Fine adjustment knobs

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14
Q

Holds the eyepieces in place

A

Eyepiece tube

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15
Q

o Also known as eyepiece
o Remagnifies the imagine formed by your
objective lens
o 10x

A

Ocular lens

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16
Q

o Allows us to compensate the difference in
eyesight between the left and right eye

A

Diopter Adjustment ring

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17
Q

o Scanner - 4x;
o LPO - 10x;
o HPO - 40x;
o OIO - 100x;

A

red
yellow
blue
white

Objective lens

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18
Q

is commonly used in
parasitology

A

LPO and HPO

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19
Q

is used in parasitology when
determining the presence of parasites in
the blood (e.g. malarial parasites)

A

OIO

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20
Q

o Used to collect and focus the light from the
light source/illuminator

A

Condenser

21
Q

o Located above the condense and below the
stage
o To control the amount of light that will reach
the specimen

A

Iris diaphragm

22
Q

Source of light that is located at the base of
the microscope

A

Illuminator/ Light Source

23
Q

Allows us to regulate the intensity of light in
the illuminator

A

Light intensity adjustment knob

24
Q

Controls the flow of current that allows the
light source to be turned on or off

A

Light siwtch

25
Q

Distance between the left and the right eye

A

Interpupillary distance

26
Q

When we change from one objective lens to
another, the image will either stay in focus or close
enough to being unfocused

A

Parfocal

27
Q

When we change from one objective lens to
another, the image of the object will stay at the
center

A

Parcentered

28
Q

This is the ability of the objective lens to view
adjacent structures as separate objects

A

Resolving power

29
Q
  • Distance between the objective lens and the
    closest surface of the cover slip
  • Increasing the objective lens will decrease the
    working distance
A

Working distance

30
Q
  • Multiply the magnification power of the eyepiece to
    the objective lens that you are using
  • Eg. Using LPO: 10 x 10 = 100
A

Total magnification power

31
Q

It is called brightfield compound microscope
because the magnified objects under the
microscope appear dark against a bright
background

A

BRIGHTFIELD COMPOUND MICROSCOPE

32
Q

because the image that we see is being
magnified by two lenses (magnified twice: ocular
lens and objective lens)

A

COMPOUND

33
Q

Give me the 5 microscope dos and dont’s

A
  1. Keep the microscope covered with a clean plastic
    or cloth cover when not in use.
  2. Use a soft cloth dampened with xylene to clean
    immersion oil from the OIO. Then, polish with a
    clean lint free cloth.
  3. When preparing the microscope for storage, make
    sure that the objective lens facing the stage is
    scanner and the stage is brought down
  4. Manipulate the light intensity adjustment knob to
    reduce the light emitted by the illuminator to
    minimum; then, turn off the light switch and unplug.
34
Q

Give me the SLIDES DO’S AND DON’TS

A
  1. Reject slides that are:
    ▪ imperfectly cleaned
    ▪ surface scratch or notched edge
    ▪ frosted appearance
  2. Always handle cleaned slides by the edges.
  3. Used slides should initially be placed in water
    containing a detergent. Then, slides will be cleaned
    one by one and transferred to a fresh solution of
    detergent. Rinse then dry with a clean cotton cloth
  4. Slides are best stored in a dry place.
  5. It is recommended that cleaned slides be stored by
    10s
35
Q

Also known as pseudoparasites
- Structures that resemble parasite but in reality are
not
(4)

A

ARTIFACTS AND CONFUSERS
Disease process
Medications
Dietary habits
Specimen contamination

36
Q

It is a technique used to measure the size of
microscopic objects

A

Micrometry

37
Q

is an essential tool for characterization,
classification, and identification

A

The size of a parasite:

38
Q

Give me the principle of micrometer

A

Calibration of the ocular micrometer using
the stage micrometer

39
Q

A glass disc with 100 equal divisions or lines on it
but with no absolute value and it is placed in the
ocular of the microscope

A

Ocular micrometer

40
Q

are flat glass disk on which a
line scale divided into 100 small division has been
etched

A

ocular micrometer

41
Q

Distance between is _______, hence
calibration is a must with the aid of an
instrument known as ___________

A

unknown - stage micrometer

42
Q
  • Used to calibrate ocular micrometer
  • It looks like a microscope slide but has a standard
    scale etched into it
  • The smallest division are 0.1mm in length (0.01 mm
    = 10um)
A

Stage micrometer

43
Q

The lens that is within the eyepiece of the light microscope is called the:
scanning
low power
high power
ocular

A

ocular

44
Q
  1. The wheel under the stage that adjusts the amount of light is called the:
    coarse knob
    body tube
    stage clip
    diaphragm
A

diaphragm

45
Q
  1. To focus a specimen, it is best to start with which objective:
    high power
    low power
    scanning
    ocular
A

scanning

46
Q
  1. When using the high power objective, you should not adjust the:
    coarse focus
    fine focus
    diaphragm
    stage clips
A

coarse focus

47
Q
  1. The scanning, low, and high power objectives are mounted on the:
    revolving nosepiece
    stage
    body tube
    eyepiece
A

revolving nosepiece

48
Q
  1. A microscope has a 4x ocular lens and a 10x objective, what is this microscope’s total magnification?
    4x
    14x
    40x
    400x
A

40X

PARASITOLOGY LAB (5 decks)

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