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Parasitology (LAB) > Microscope > Flashcards

Microscope Flashcards

1
Q

specimen is positioned properly on the stage of a microscope & illuminated by a light source will be magnified by a two-lens system

A

COMPOUND

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2
Q

magnified objects appear as dark objects against a bright background

A

BRIGHTFIELD

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3
Q

must exist between the magnified object & brightfield background for the objects to be visible

A

SUFFICIENT CONTRAST

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4
Q

Components of a Microcope:

A
  1. Lens System
  2. Illumination System
  3. Body
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5
Q

Further magnify the object for viewing

A

EYEPIECES OR OCULARS with 10x lenses

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6
Q

lenses magnify the intermediate image formed by the objective lenses

A

EYEPIECES OR OCULARS with 10x lenses

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7
Q

limit the area of visibility

A

EYEPIECES OR OCULARS with 10x lenses

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8
Q

should not be interchanged with other models of microscopes

A

EYEPIECES OR OCULARS with 10x lenses

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9
Q

used to adjust the lateral separation of the eyepieces for each individual

A

INTERPUPILLARY CONTROL

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10
Q

connects the eyepieces with the objective

A

OPTICAL TUBE

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11
Q

What is the standard length for the optical tube?

A

160 mm

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12
Q

gives structural site of attachment for the revolving nosepiece

A

NECK, OR ARM

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13
Q

the main vertical support of the microscope

A

STAND

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14
Q

supports: stage assembly, together w/ the condenser & base

A

STAND

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15
Q

holds the objectives

A

REVOLVING NOSEPIECE

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16
Q

allows easy rotation from one objective lens to another w/ a specific power of magnification

A

REVOLVING NOSEPIECE

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17
Q

Objective lens barrel are engraved w/ the power of magnification

A

`FOUR OBJECTIVE LENSES

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18
Q

Power magnification of the four objective lenses

A
  1. Power of magnification
  2. Numerical aperture (NA)
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19
Q

the larger ____, the greater the resolution, or ability to distinguish between fine details of two closely situated objects

A

NA

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20
Q

the larger _________, the smaller the viewing field

A

magnification

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21
Q

Standard powers of magnification:

A

Low power: 10X
High power: 40X
Oil immersion: 100X
lpo & hp: urine sediment examination

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22
Q

total magnification is calculated

A

10x (eyepiece) by 100x (oil immersion) = 1000x total magnification

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23
Q

shorter the wavelength of light, the greater the resolving power of the microscope

24
Q

supports the prepared microscope slide to be reviewed

25
Q

engages coarse control (one direction)

A

FOCUS CONTROLS

26
Q

engages fine control (moved in opposite direction)

A

FOCUS CONTROLS

27
Q

Order of usage for focus controls:

A

Engage Coarse adjustment 1st then fine-tune w/ fine adjustment

28
Q

gathers, organizes, & directs the light

29
Q

focuses light on specimen

30
Q

uniform illumination

31
Q

controls the angle (NA) & amount of light

32
Q

For best resolution, Iris is used:

A

Fully open
Partially open
Fully closed

33
Q

For best resolution, Iris is used:

sacrificed of image contrast

A

Fully open

34
Q

For best resolution, Iris is used:

slight improvement in image contrast

A

Partially open

35
Q

For best resolution, Iris is used:

Loss of resolution

A

Fully closed

36
Q

is moved along an x- or y-axis

A

STAGE CONTROLS

37
Q

when open: allows maximally sized circle of light

A

STAGE CONTROLS

38
Q

when partially open: assists in centering the condenser apparatus

A

STAGE CONTROLS

39
Q

2 types of Brightfield Illumination:

A
  1. Critical illumination
  2. Koehler (Köhler)System
40
Q

results in increased but uneven brightness

A

Critical Illumination

41
Q

light source focused on specimen

A

Critical Illumination

42
Q

light source & condenser are properly aligned

A

Koehler (Köhler)System

43
Q

evenly distributed brightness across the specimen

A

Koehler (Köhler)System

44
Q

turns on light

A

RHEOSTAT/LIGHT CONTROL KNOB/LEVER

45
Q

regulate brightness of light needed in visualizing specimen

A

RHEOSTAT/LIGHT CONTROL KNOB/LEVER

46
Q

Care of the Microscope:

A
  1. Carry microscope with 2 hands, support base with one hand
  2. Always hold microscope in a vertical position
  3. Only clean optical surfaces w/ a good quality lens tissue & commercial lens cleaner
  4. Do not use the 10X & 40X objectives with oil
  5. Clean the oil immersion lens after use
  6. Always remove slides w/ the low-power objective raised
  7. Store the microscope w/ the low-power objective in position & the stage centered
47
Q

used to increase resolving power of light microscopes

A

IMMERSION OIL

48
Q

increase the magnification & contrast of a specimen under a microscope

A

IMMERSION OIL

49
Q

translates differences in phase of the light transmitted through or reflected by the object into differences of intensity of the image

A

PHASE CONTRAST MICROSCOPE

50
Q

used for observing specimens that have not been stained & are in their natural state

A

PHASE CONTRAST MICROSCOPE

51
Q

capable of providing information on absorption color & optical path boundaries between minerals of differing refractive indices

A

POLARIZED LIGHT MICROSCOPE

52
Q

can distinguish between isotropic & anisotropic substances

A

POLARIZED LIGHT MICROSCOPE

53
Q

takes advantage of oblique illumination to enhance contrast in specimens that are not imaged well under normal conditions

A

DARKFIELD MICROSCOPE

54
Q

designed for directly studying the surfaces of solid objects

A

SCANNING ELECTRON MICROSCOPE (SEM)

55
Q

used to view thin specimens (tissue sections, molecules, etc) through which electrons can pass generating a projection image

A

TRANSMISSION ELECTRON MICROSCOPE (TEM)

Parasitology (LAB) (3 decks)

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