Microscope Flashcards
specimen is positioned properly on the stage of a microscope & illuminated by a light source will be magnified by a two-lens system
COMPOUND
magnified objects appear as dark objects against a bright background
BRIGHTFIELD
must exist between the magnified object & brightfield background for the objects to be visible
SUFFICIENT CONTRAST
Components of a Microcope:
- Lens System
- Illumination System
- Body
Further magnify the object for viewing
EYEPIECES OR OCULARS with 10x lenses
lenses magnify the intermediate image formed by the objective lenses
EYEPIECES OR OCULARS with 10x lenses
limit the area of visibility
EYEPIECES OR OCULARS with 10x lenses
should not be interchanged with other models of microscopes
EYEPIECES OR OCULARS with 10x lenses
used to adjust the lateral separation of the eyepieces for each individual
INTERPUPILLARY CONTROL
connects the eyepieces with the objective
OPTICAL TUBE
What is the standard length for the optical tube?
160 mm
gives structural site of attachment for the revolving nosepiece
NECK, OR ARM
the main vertical support of the microscope
STAND
supports: stage assembly, together w/ the condenser & base
STAND
holds the objectives
REVOLVING NOSEPIECE
allows easy rotation from one objective lens to another w/ a specific power of magnification
REVOLVING NOSEPIECE
Objective lens barrel are engraved w/ the power of magnification
`FOUR OBJECTIVE LENSES
Power magnification of the four objective lenses
- Power of magnification
- Numerical aperture (NA)
the larger ____, the greater the resolution, or ability to distinguish between fine details of two closely situated objects
NA
the larger _________, the smaller the viewing field
magnification
Standard powers of magnification:
Low power: 10X
High power: 40X
Oil immersion: 100X
lpo & hp: urine sediment examination
total magnification is calculated
10x (eyepiece) by 100x (oil immersion) = 1000x total magnification