microRNA and cancer

Question 1

what is elF4E and what does it bind?

Answer 1

The 5’ cap protein. binds to elF4G which brings in the poly A tail. This is needed for recruitment of the 40s ribosome and initiation of translation.

Question 2

Which proteins to miRNAs use to bind mRNAs?

Answer 2

GW182 proteins

Question 3

Which miRNA act as tumour supressors?

Answer 3

miR-15a,16-1,29a,b, and c, let-7. Target BCl2, cdk6, ras

Question 4

Which miRNAS act as oncogenes?

Answer 4

miR-17-92, 155 and 21. Target PTEN, BIM SHIP1 etc.

Question 5

What can miRNA profiling be used for?

Answer 5

1. identify tissue of origin,
2. distinguish tumour subtypes
3. study SNPs in miR genes, binding sites, processing pathways to predict cancer.
4. Prolifiling exosomal miR in the blood=no invasive procedure.
5. differentiate normal and tumour tissue.