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MICROPARA > MICROPARA-PRLMS-LABQUIZ-1 > Flashcards

MICROPARA-PRLMS-LABQUIZ-1 Flashcards

- Exercise 1, Compound Microscope // Exercise 2, Magnification

1
Q

The smallest object that the eye can see at a distance of ___________ is about ________________

A

250 mm ; 0.07-0.14 mm

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2
Q

This allows us to see minute organisms

A

Microscope

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3
Q

What are the two types of Microscope

A

Light microscope and Electron microscope

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4
Q

A light microscope is classified as _____________ if they have a short focal length, held close to the eye and magnify objects only up to ______________

A

Simple ; 300x

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5
Q

A light microscope that employs two separate lenses, an ocular, and an objective, in order to achieve 2-5 times greater magnification

A

Compound Microscope

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6
Q

Other types of microscope:

  • microscope field is brightly lighted
  • specimen to be observed is dark due to its ability to absorb or refract some of the incident light
A

bright-field microscope

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6
Q

Other types of microscope:

  • specimen appears luminous against a dark background since it reflects some of the incident light in all directions
A

dark-field microscope

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6
Q

Other types of microscope:

  • uses the property of certain chemical substances that release light at a different wavelength when exposed to UV rays
A

fluorescent microscope

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7
Q

Other types of microscope:

  • uses UV light (greater resolution and magnification)
  • to detect or measure substances in specimens of living tissues that are known to absorb UV light
  • imaged formed is recorded with the use of cameras or a television screen
A

ultraviolet (UV) microscope

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8
Q

While using fluorescent microscope, the specimen, such as bacteria and other microorganisms are stained with?

A

fluorescent stain

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9
Q

Other types of microscope:

  • utilizes the refraction that occurs when light passes from one medium into another of different density
  • useful in studying the internal structure of microorganisms
A

phase-contract microscope

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10
Q

MICROSCOPE PARTS

It keeps the microscope steady at any positions of the stage

A

BASE

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11
Q

MICROSCOPE PARTS

It is fastened to the base through the inclination joint and it permits the adjustment of the stage to a desired angle

A

ARM

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12
Q

MICROSCOPE PARTS

It reflects the light into the condenser

A

MIRROR

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13
Q

MICROSCOPE PARTS

Two types of mirrors

A

Plane mirrors / Concave mirrors

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14
Q

MICROSCOPE PARTS

Use of Plane mirrors

A

for natural light

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15
Q

MICROSCOPE PARTS

Use of concave mirrors

A

for artificial light

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16
Q

MICROSCOPE PARTS

Regulates the amount of light entering the condenser

A

IRIS DIAPHRAGM

17
Q

MICROSCOPE PARTS

It concentrates the light rays received from the mirror and sends them to the objective

A

CONDENSER

18
Q

MICROSCOPE PARTS

A horizontal platform upon which the specimen to be examined is placed

A

STAGE

18
Q

MICROSCOPE PARTS

Found at the center of the stage

A

CIRCULAR APERTURE

19
Q

MICROSCOPE PARTS

It holds the slide in place on the stage

A

STAGE CLIPS

20
Q

MICROSCOPE PARTS

A part of the optical system of the microscope which produces the specimen’s initial magnified image (real) within the body tube

A

Objective

21
Q

MICROSCOPE PARTS

The three objectives of the microscope

A

Dry low power
Dry high power
Oil immersion objective

22
Q

MICROSCOPE PARTS

These parts are for Manipulation, adjustment, support, or protection

A

Mechanical Parts

23
Q

MICROSCOPE PARTS

These parts are for illumination and magnifying

A

Optical Parts

24
Q

MICROSCOPE PARTS

Two types of optical parts

A

Illuminating parts
Magnifying parts

24
Q

FEATURES OF THE OBJECTIVES:

  • It is an optical constant of the lens system
  • It is the distance from the center of the lens where parallel rays entering the lens are brought to a focus
A

Focal Length (mm)

25
Q

FEATURES OF THE OBJECTIVES:

A property to recognize features of a specimen that are close to each other as separate distinct

Is dependent on wavelength of light

A

Resolving Power

26
Q

FEATURES OF THE OBJECTIVES:

A measure of the resolving power of an objective

A

Numerical Aperture (N.A.)

27
Q

FEATURES OF THE OBJECTIVES:

Objectives are optically and mechanically designed

Distance between the specimen and the aerial image is always constant

A

Parfocal

28
Q

MICROSCOPE PARTS

Where parfocal objectives are attached, allows convenient shifting of the objectives

A

REVOLVING NOSE PIECE

29
Q

MICROSCOPE PARTS

A hollow cylindrical tube through which light passes from the objective to the eyepiece

A

BODY TUBE

30
Q

MICROSCOPE PARTS

The upper portion of the body tube

A

DRAW TUBE

31
Q

MICROSCOPE PARTS

Where the specimen is viewed

A

EYEPIECE OR OCULAR

32
Q

Where is the term ‘Compound Microscope’ come from?

A

Derived from the fact that the specimen is magnified twice, first by the objective and second by the eyepiece

33
Q

What do you call the final image formed?

A

Virtual Image

34
Q

The total magnification of a Compound Microscope

A

1000x

35
Q

MICROSCOPE PARTS

It is used to bring the object into approximate focus

A

COARSE FOCUS KNOB / COARSE ADJUSTMENT KNOB

36
Q

MICROSCOPE PARTS

It is for maximum definition

A

FINE FOCUS KNOB

37
Q

NUMERICAL APERTURES

OIL

A

1.25

38
Q

NUMERICAL APERTURES

HPO

A

0.65

39
Q

NUMERICAL APERTURES

LPO

A

0.25

MICROPARA (3 decks)

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