MICROPARA-PRLMS-LABQUIZ-1 Flashcards
- Exercise 1, Compound Microscope // Exercise 2, Magnification
The smallest object that the eye can see at a distance of ___________ is about ________________
250 mm ; 0.07-0.14 mm
This allows us to see minute organisms
Microscope
What are the two types of Microscope
Light microscope and Electron microscope
A light microscope is classified as _____________ if they have a short focal length, held close to the eye and magnify objects only up to ______________
Simple ; 300x
A light microscope that employs two separate lenses, an ocular, and an objective, in order to achieve 2-5 times greater magnification
Compound Microscope
Other types of microscope:
- microscope field is brightly lighted
- specimen to be observed is dark due to its ability to absorb or refract some of the incident light
bright-field microscope
Other types of microscope:
- specimen appears luminous against a dark background since it reflects some of the incident light in all directions
dark-field microscope
Other types of microscope:
- uses the property of certain chemical substances that release light at a different wavelength when exposed to UV rays
fluorescent microscope
Other types of microscope:
- uses UV light (greater resolution and magnification)
- to detect or measure substances in specimens of living tissues that are known to absorb UV light
- imaged formed is recorded with the use of cameras or a television screen
ultraviolet (UV) microscope
While using fluorescent microscope, the specimen, such as bacteria and other microorganisms are stained with?
fluorescent stain
Other types of microscope:
- utilizes the refraction that occurs when light passes from one medium into another of different density
- useful in studying the internal structure of microorganisms
phase-contract microscope
MICROSCOPE PARTS
It keeps the microscope steady at any positions of the stage
BASE
MICROSCOPE PARTS
It is fastened to the base through the inclination joint and it permits the adjustment of the stage to a desired angle
ARM
MICROSCOPE PARTS
It reflects the light into the condenser
MIRROR
MICROSCOPE PARTS
Two types of mirrors
Plane mirrors / Concave mirrors
MICROSCOPE PARTS
Use of Plane mirrors
for natural light
MICROSCOPE PARTS
Use of concave mirrors
for artificial light
MICROSCOPE PARTS
Regulates the amount of light entering the condenser
IRIS DIAPHRAGM
MICROSCOPE PARTS
It concentrates the light rays received from the mirror and sends them to the objective
CONDENSER
MICROSCOPE PARTS
A horizontal platform upon which the specimen to be examined is placed
STAGE
MICROSCOPE PARTS
Found at the center of the stage
CIRCULAR APERTURE
MICROSCOPE PARTS
It holds the slide in place on the stage
STAGE CLIPS
MICROSCOPE PARTS
A part of the optical system of the microscope which produces the specimen’s initial magnified image (real) within the body tube
Objective
MICROSCOPE PARTS
The three objectives of the microscope
Dry low power
Dry high power
Oil immersion objective
MICROSCOPE PARTS
These parts are for Manipulation, adjustment, support, or protection
Mechanical Parts
MICROSCOPE PARTS
These parts are for illumination and magnifying
Optical Parts
MICROSCOPE PARTS
Two types of optical parts
Illuminating parts
Magnifying parts
FEATURES OF THE OBJECTIVES:
- It is an optical constant of the lens system
- It is the distance from the center of the lens where parallel rays entering the lens are brought to a focus
Focal Length (mm)
FEATURES OF THE OBJECTIVES:
A property to recognize features of a specimen that are close to each other as separate distinct
Is dependent on wavelength of light
Resolving Power
FEATURES OF THE OBJECTIVES:
A measure of the resolving power of an objective
Numerical Aperture (N.A.)
FEATURES OF THE OBJECTIVES:
Objectives are optically and mechanically designed
Distance between the specimen and the aerial image is always constant
Parfocal
MICROSCOPE PARTS
Where parfocal objectives are attached, allows convenient shifting of the objectives
REVOLVING NOSE PIECE
MICROSCOPE PARTS
A hollow cylindrical tube through which light passes from the objective to the eyepiece
BODY TUBE
MICROSCOPE PARTS
The upper portion of the body tube
DRAW TUBE
MICROSCOPE PARTS
Where the specimen is viewed
EYEPIECE OR OCULAR
Where is the term ‘Compound Microscope’ come from?
Derived from the fact that the specimen is magnified twice, first by the objective and second by the eyepiece
What do you call the final image formed?
Virtual Image
The total magnification of a Compound Microscope
1000x
MICROSCOPE PARTS
It is used to bring the object into approximate focus
COARSE FOCUS KNOB / COARSE ADJUSTMENT KNOB
MICROSCOPE PARTS
It is for maximum definition
FINE FOCUS KNOB
NUMERICAL APERTURES
OIL
1.25
NUMERICAL APERTURES
HPO
0.65
NUMERICAL APERTURES
LPO
0.25
