Microbiology Pt. 1

Question 1

Characteristics of Nutrient Agar

Answer 1

contains carbohydrates, amino acids, vitamins, nitrogen source, and salts

Question 2

Nutrient Agar selective/differential

Answer 2

Neither

Question 3

Growth of Nutrient Agar

Answer 3

Bacteria, yeast, molds

Question 4

Characteristics of MacConkey

Answer 4

contains bile salts and crystal violet dye, inhibit Gram (+) growth.
contains neutral red pH indicators
low pH = red
high pH = yellow

Question 5

MacConkey selective/differntial

Answer 5

both
selective: inhibits Gram (+) growth
differential: ability to ferment lactose

Question 6

Growth of MacConkey

Answer 6

Gram (-) bacteria
pink/red: able to ferment lactose
Waite/colorless/golden: unable to ferment lactose

Question 7

Characteristics of Eosin Methylene Blue (EMB)

Answer 7

contains eosin and methylene blue dyes, inhibits Gram (+) growth

Question 8

Eosin Methylene Blue (EMB) selective/differential

Answer 8

Both
selective: inhibits Gram (+) growth
differential: ability to ferment lactose

Question 9

Growth of EMB

Answer 9

Gram (-) bacteria
metallic green: E coli
pink/purple/black: able to ferment lactose
colorless: unable to ferment lactose

Question 10

Characteristics of Blood Agar

Answer 10

contains sheeps blood:
fastidious bacteria that require blood can grow
contains general nutrients:
any kind of bacteria can grow

Question 11

Blood Agar selective/differential

Answer 11

non-selective
differential: identify organisms that have hemolytic activity

Question 12

Growth of Blood Agar

Answer 12

colony surrounded by green color: hemolysis (incomplete lysis and digestion)
colony surrounded by white/clear: B-hemolysis (complete lysis/digestion)
Agar under/around colony is unchanged: y-hemolysis (no hemolysis)

Question 13

When bacteria undergo cell division, they are clones of their patent cell. True/False

Answer 13

True

Question 14

What are 2 key differences between Prokaryotes and Eukaryotes?

Answer 14

Prokaryotes contain a nucleoid region and eukaryotes do not. Eukaryotes contain a nucleus and prokaryotes do not

Question 15

Media that prevents the growth of some type of microorganisms and permits the growth of other is said to be…

Answer 15

selective

Question 16

Media that allows you to tell whether or not a colony has a particular property is called…

Answer 16

different

Question 17

Nutrient Agar

Answer 17

contains all the nutrients necessary for growth

Question 18

Eosin Methylene Blue (EMB)

Answer 18

eosin, methylene blue

Question 19

Blood Agar

Answer 19

blood

Question 20

Elongated, rod-shaped bacteria are called…

Answer 20

bacill

Question 21

How does sterilization method prevent growth?

Answer 21

Microorganisms grow rapidly in culture media, and correct transfer techniques and the use of sterile reagents are usually enough to prevent contamination

Question 22

Gram-positive bacteria…..

Answer 22

have a cell membrane and a thick cell wall

Question 23

Gram-negative bacteria have….

Answer 23

a cell membrane, a thin cell wall, and an outer phospholipid membrane

Question 24

Anaerobic bacteria

Answer 24

only grows in absence of oxygen

Question 25

Photoautotroph bacteria

Answer 25

produces energy using sunlight

Question 26

Aerobic bacteria

Answer 26

requires oxygen to grow

Question 27

Facultative bacteria

Answer 27

survives best a low oxygen

Question 28

Chemoautotroph bacteria

Answer 28

obtains its energy through chemicals

Question 29

Pathogenic bacteria

Answer 29

causes disease