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Bacte Lab (Finals) > Microbiology of Water > Flashcards

Microbiology of Water Flashcards

1
Q

Pathogenic intestinal infections (BC kaon ug TaPa)

A
  1. Bacillary dysentery
  2. Cholera
  3. Typhoid Fever
  4. Paratyphoid Fever
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2
Q

Waterborne enteric bacterial pathogens (Sige’g Swim C Vibrio)

A
  1. Shigella dysentery
  2. Salmonella typhi
  3. Campylobacter jejuni
  4. Vibrio cholerae
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3
Q

Why is water microbiology not a reliable indicator of fecal pollution in tropic and subtropic areas?

A

Soil may naturally contain high levels of E coli.

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4
Q

3 tests for qualitative analysis of water

A
  1. Presumptive Test
  2. Confirmatory Test
  3. Completed Test
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5
Q

This test is specific for the detection of coliform

A

Presumptive Test

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6
Q

A specialized broth utilized under Presumptive Test

A

Lactose Fermentation Broth

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7
Q

This tube is placed in a broth to detect deterioration of the broth before inoculation and identify denitrification by organisms that produce gas by alternate pathways.

A

Durham Tube

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8
Q

Water is examined to detect this bacterium that indicates fecal pollution.

A

Escherichia coli

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9
Q

These bacteria are indicators of fecal contamination

A

Coliform bacteria

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10
Q

Ferments lactose with the production of acid and gas

A

gram-negative, non-spore-forming bacilli

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11
Q

This medium facilitates the detection of coliform bacteria because these bacteria are capable of using lactose as a carbon source.

A

Lactose fermentation broth

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12
Q

This tube is utilized for gas collection

A

Durham tube

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13
Q

Under presumptive test, tubes of the lactose medium are to be inoculated with how many aliquots of water sample? How many tubes are specified in each group?

A

10 mL, 1 mL, and 0.1 mL;
5 Tubes

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14
Q

The greater the number of tubes per group, the greater the _____________ of the test.

A

sensitivity

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15
Q

The presence of coliform bacteria is evidenced in its development

A

gas

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16
Q

This test enables microbiologists to obtain some idea of the number of coliform organisms present.

A

Most Probable Number (MPN) Test

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17
Q

The presence of a positive or doubtful presumptive test suggests that the water sample is …

A

nonpotable

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18
Q

Selective and differential media for confirmed test

A

Eosin-methylene blue (EMB)
Endo Agar

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19
Q

This media inhibits the growth of gram-positive organisms because of its dye.

A

Eosin methylene blue
(Dye: methylene blue)

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20
Q

In the presence of an acid environment, EMB forms a complex that precipitates out onto the coliform colonies, producing 2 appearances.

A
  1. Dark centers
  2. Green metallic sheen

The reaction is characteristic for E coli.

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21
Q

Dye contained in the Endo agar responsible for the formation of a dark pink complex that turns E coli. colonies and the surrounding medium into pink.

A

Fuchsin

22
Q

The EMB agar plate from a positive tube is incubated for and at …

A

For 24 hours; at 37 oC

23
Q

Indicates positive completed test

A

Gram-negative short bacilli

24
Q

The final analysis of the water sample used to examine the coliform colonies that appeared on the EMB or Endo agar plates.

A

completed test

25
Q

This agency has the government mandate to protect the nation’s waterways and terrestrial environments from human contamination or damage.

A

Environmental Protection Agency (EPA)

26
Q

The absence of E. coli indicates

A

negative test- water is not contaminated with fecal wastes and is therefore potable

27
Q

Used in quantitative analysis for the purpose of counting the total coliforms, fecal coliforms, and E coli.

A

Most Probable Number (MPN)

28
Q

Advantages of MPN

A
  1. Inoculated greater amounts of sample
  2. Has higher sensitivity than plate count method
  3. Versatile
29
Q

Presumptive test for coliform under quantitative analysis utilizes

A

Lauryl Sulphate Tryptose (LST)

30
Q

The LST is incubated at and for …

A

At 35 oC; for 24 hours

31
Q

Selective medium for quantitative analysis

A

Brilliant Green Broth (BGB)

32
Q

A medium that inhibits noncoliforms with the presence of lactose and 2% bile

A

Brilliant Green Broth

33
Q

Growth is verified by

A

turbidity or cloudiness

34
Q

Indicates gas production

A

bubbles in Durham tube

35
Q

The membrane filters are capable of retaining organisms larger than …

A

larger than 0.45 um

36
Q

Trapped microoganisms are transferred in 50-mm petri dish containing …

A

adsorbent pad

37
Q

Agar medium used for Membrane Filter Method

A

Endo Agar

38
Q

In the endo agar, coliform growth has this appearance

A

Red or Metallic colonies

39
Q

Formula for total colony count

A

Total Colony Count = colony count x DF/ mL of sample used multiplied by 100

40
Q

Presumptively identifies Enterobacteriaceae and verotoxin producing E. coli

A

4-Methylumbelliferyl-B-d-glucuronide (MUG) Test

41
Q

Principle for MUG Test

A

B-d-glucopyranosid-uronic with the enzyme B-d-glucuronidase is separated into Alycons and D-glucuronic acid + 4-methylumbelliferyl-B-d-glucuronide would yield 4-methylumbelliferyl moiety = Blue Fluorescence

42
Q

MUG test is observed in

A

366-nm UV

43
Q

Differentiates microoganisms based on metabolic reaction in litmus milk

A

litmus milk medium

44
Q

What are observed in the litmus milk medium?

A
  1. Fermentation
  2. Clot formation
  3. Digestion
  4. Formation of gas
45
Q

Appearance of Indicator Litmus Dye when ph change to acid

A

Pink, mauve [Record: Acid (A)]

46
Q

Appearance of Indicator Litmus Dye when pH change to Alkaline

A

Blue [Record: Alkaline (K)]

47
Q

Appearance of Indicator Litmus Dye when pH has no change

A

Purple

48
Q

Appearance of Indicator Litmus Dye when pH change to independent as a result of reduction of the indicator

A

white [Record: Decolorized]

49
Q

Consistency of milk when pH is acid or alkaline

A

Coagulation or clot

50
Q

Consistency of milk when pH is acid

A

Dissolution of clot with clear, grayish, watery fluid, and a shrunken, insoluble pink clot

[Record: Digestion]

51
Q

Consistency of milk when pH is alkaline

A

Dissolution of clot with grayish, watery fluid and a clear, shrunken, insoluble blue clot

[Record: Peptonization]

Bacte Lab (Finals) (1 decks)

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