Microbiology of the Root Canal System Flashcards

1
Q

The root canal system provides an ideal environment for?

A

bacterial colonization

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2
Q

T or F:
The root canal system is warm, moist, and nutritious (has lots of substrate) and has a variable but largely anaerobic climate.

A

True

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3
Q

The root canal system is largely protected from the host defenses due to..?

A

lack of circulation in a diseased pulp

“Privileged Sanctuary”

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4
Q

What basic science is most closely associated with the practice of Endodontics?

A

Microbiology

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5
Q

How were anaerobic bacteria successfully cultured from the canal system? Who discovered this technique?

A

1- By using a special “anaerobic glove box” technique.

2-Sundqvist

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6
Q

What did Sundqvist prove?

A

He proved that many of the pathological bacteria found in the root canal system are black pigmented anaerobic gram negative bacteria

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7
Q

What do gram negative bacteria release?

A

Lipopolysaccharides (also known as endotoxins). They induce fever, collegenolysis, and osteolysis.

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8
Q

Which bacteria is the most frequent in endo infections?

A

Prevotella nigrescens

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9
Q

The canal walls/spaces are conducive to formation of…?

A

biofilm (complex, colonized communities of bacteria)

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10
Q

T or F:

One single microorganism can be responsible for apical periodontitis.

A

False

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11
Q

Progression of Root Canal infections? (steps 1-3)

A

1- carious lesion or trauma opens tubules to bacterial invasion

2- bacteria inflame pulp locally

3- inflammation may overcome pulpal defenses and localized abscesses may form in coronal pulp

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12
Q

Progression of Root Canal infections? (steps 4-7)

A

4- infection increases in pulp and necrosis begins

5- necrosis involves entire root canal system

6- infection uses “portals of exit” to invade periradicular tissues (apical periodontitis)

7- periradicular infection occurs beyond apex (apical abscess)

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13
Q

“Portals of Exit” for infection?

A

apical foramen and lateral canals

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14
Q

Biofilm is defined as a ____ microbial community characterized by cells that are firmly attached to a surface and enmeshed in a self produced matrix of extracellular polymeric substance.

A

multicellular

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15
Q

What should we think when we hear biofilm?

A

think complex, variable, and constantly changing bacterial infection

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16
Q

Biofilm must accomplish these 6 things to cause disease within the root canal system:

A
  1. Microorganism must adhere to host surfaces
  2. Obtain nutrients from the host
  3. Multiply
  4. Invade tissue
  5. Overcome host defenses
  6. Induce tissue damage
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17
Q

The longer the infection exists within the root canal system, the more _____ is consumed by bacterial activity and the more anaerobic becomes the root canal system.

A

oxygen

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18
Q

Chronic infections within the root canal system tend to favor…?

A

anaerobic bacterial flora

19
Q

Some examples of anaerobic gram negative bacteria?

A
porphyromonas
prevotella
peptostreptococcus
streptococcus
actinomyces
olsenella
propionibacterium
20
Q

How do we destroy bacteria?

A

kill them directly or starve them out

21
Q

How and what is used in endo to destroy bacteria?

A

Cleaning and shaping with 8.3% Sodium Hypochlorite (NaOCl) and intra-canal medicaments

22
Q

Biofilm becomes more varied, complex, and difficult to control when the tooth is further challenged by…?

A

salivary contact or operative contamination

23
Q

How to avoid salivary contact and operative contamination?

A

Avoid leakage of rubber dam and avoid iatrogenic contamination during treatment

24
Q

Which areas in the tooth are inaccessible to instrumentation?

A

Canals. They have many ramifications, anastomoses, fins, webs, and bifurcations where microorganisms can hide.

25
Q

T or F:

Flies never reach ALL spaces in the complex pulpal system

A

True

26
Q

Correct use of ____ is extremely important in elimination microorganisms in root canal systems.

A

NaOCl (sodium hypochlorite)

27
Q

What are the 6 functions of sodium hypochlorite (NaOCl) in root canal systems?

A
1- disinfects over time
2- dilutes and inactivates toxins
3- dissolves substrate over time
4- dissolves necrotic tissue over time
5- flushes and floats out debris
6- lubricates canal
28
Q

NaOCl degrades rapidly to ___ in the canal, therefore must be replenished @ 3-5 minutes.

A

NaCl (salt water)

29
Q

How much NaOCl is typically used as irrigant per visit?

A

about 10-12 cc

30
Q

NaOCl must be in contact with shaped canal a minimum of…?

A

30 minutes after canal enlarged to #30 or larger.

31
Q

T or F:

A canal smaller than #30 will rarely if ever allow any irrigant to reach the apical 1/3 of the canal.

A

True

32
Q

When are medications placed within the canal system?

A

Between appointments. Intended to increase local anti-microbial action and to further decrease the microbial challenge within the root canal system.

33
Q

Examples of toxic, damaging, and sometimes carcinogenic medicaments used historically?

A

CMCP,
Formocresol,
Cresatin,
Beachwood Creosote

34
Q

What is the universally accepted intracanal medication of choice (especially in necrotic cases)?

A

Calcium Hydroxide (CaOH)

35
Q

What is the pH of CaOH?

A

between 11 and 12

36
Q

Advantages for using CaOH?

A
  • Discourages most microbial growth
  • Long lasting
  • No reported allerfic responses
  • Easy to apply and remove as a paste
37
Q

What is the name of the CaOH product available in clinic?

A

Calasept

38
Q

In endo, the use of antibiotics is ____ to relieve pain.

A

USELESS

39
Q

Systemic antibiotics are useful only in…?

A

Acute periradicular infections (swelling and fever) or for a patient who is immunologically supressed

40
Q

T or F:
Healthy patients without systemic signs and symptoms of infection but with symptomatic pulpitis, symptomatic apical periodontitis, a draining sinus tract, or localized swelling DO NOT require antibiotics.

A

True.

use of antibiotics with these patients will just create resistance

41
Q

When do we use antibiotics in endodontics?

A

Medically compromised patient, pre-med when indicated, persistent or spreading infection

42
Q

Drainage is accomplished by I&D (____ & ____)

A

Incision & Drainage

43
Q

T or F:

All endo infections are polymicrobial. Mostly facultative and obligate anaerobes.

A

True and true