Microbiology of the Root Canal System Flashcards
The root canal system provides an ideal environment for?
bacterial colonization
T or F:
The root canal system is warm, moist, and nutritious (has lots of substrate) and has a variable but largely anaerobic climate.
True
The root canal system is largely protected from the host defenses due to..?
lack of circulation in a diseased pulp
“Privileged Sanctuary”
What basic science is most closely associated with the practice of Endodontics?
Microbiology
How were anaerobic bacteria successfully cultured from the canal system? Who discovered this technique?
1- By using a special “anaerobic glove box” technique.
2-Sundqvist
What did Sundqvist prove?
He proved that many of the pathological bacteria found in the root canal system are black pigmented anaerobic gram negative bacteria
What do gram negative bacteria release?
Lipopolysaccharides (also known as endotoxins). They induce fever, collegenolysis, and osteolysis.
Which bacteria is the most frequent in endo infections?
Prevotella nigrescens
The canal walls/spaces are conducive to formation of…?
biofilm (complex, colonized communities of bacteria)
T or F:
One single microorganism can be responsible for apical periodontitis.
False
Progression of Root Canal infections? (steps 1-3)
1- carious lesion or trauma opens tubules to bacterial invasion
2- bacteria inflame pulp locally
3- inflammation may overcome pulpal defenses and localized abscesses may form in coronal pulp
Progression of Root Canal infections? (steps 4-7)
4- infection increases in pulp and necrosis begins
5- necrosis involves entire root canal system
6- infection uses “portals of exit” to invade periradicular tissues (apical periodontitis)
7- periradicular infection occurs beyond apex (apical abscess)
“Portals of Exit” for infection?
apical foramen and lateral canals
Biofilm is defined as a ____ microbial community characterized by cells that are firmly attached to a surface and enmeshed in a self produced matrix of extracellular polymeric substance.
multicellular
What should we think when we hear biofilm?
think complex, variable, and constantly changing bacterial infection
Biofilm must accomplish these 6 things to cause disease within the root canal system:
- Microorganism must adhere to host surfaces
- Obtain nutrients from the host
- Multiply
- Invade tissue
- Overcome host defenses
- Induce tissue damage
The longer the infection exists within the root canal system, the more _____ is consumed by bacterial activity and the more anaerobic becomes the root canal system.
oxygen