microbiology exam 3 Flashcards
catabolism
breaking down compounds into components, releases atp, exergonic reaction through hydrolysis
anabolism
building compounds from smaller units, dehydration
enzymes
required for all reactions, applies pressure, changes shape of bond
coenzyme
activator for enzymes role
enzyme reactions
reversible, influenced by temperature, regulated by substrate concentration, product , inhibitors
atp, adp,amp
adosine triphosphate, diphosphate, monophosphate
how is atp made?
substrate level phosphorylation
kinase
donates phosphate
glucose-> glycolysis
(substrate, energy captured, product)
substate: glucose (6-C)
energy captured: 2ATP,2NADH
product: 2 pyruvate
glycolysis->pyruvate oxidation
(product, energy, process)
product: 2 CO2
energy: NADH
2 pyruvate-> 2 acetyl COA
pyruvate oxidation-> Krebs cycle
(input, bond, forms, energy)
input: 2 acetyl COA
bond: each acetyl to oxaloacetate, recycled, 4CO2 produced
forms: 2 citrate
energy: 2 ATP, 6NADH, 2 FADH2
electron transport chain & oxidative phosphorylation
( input, mechanism, energy, recycled, final electron acceptor)
input: 10NADH, 2FADH2, Oxygen
mechanism: transfer of electrons via ETC
energy: net 28ATP
recycled: NAD+ & FAD
02 final electron acceptor
pentose phospate
five carbon sugars, synthesis of nucleic acids, carbon fixation,
Enter-Doudoroff pathway
alt. glucose metabolism
different intermediates
nitrate
fully oxidized, reduced to N02-, N2, N20, pseudomonas, bacillus
sulfate
fully oxidized, reduced to H2S, desulfovibrio
carbon dioxide
fully oxidized, reduced to CH4, some archaea