Microbiology Diagnostic

Question 1

Bacillus anthracis

1 - smear show large, gram + rods in chain
2 - non heamolytic colonies forms on blood culture
3 - PCR used in bioterror attacks
4 - Direct flourscent antibody test
5 - ELISA to detect antibodies

Answer 1

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Question 2

Bacillus cereus

No laboratory diagnosis is done

Answer 2

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Question 3

Clostridium tetani

• No microbiologic or serologic diagnosis
• organism is rare isolated from wound
• C. tetani produce ((terminal spore))

Answer 3

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Question 4

Clostridium botulinum

1 - Mouse protection test

Answer 4

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Question 5

Clostridium perfringenes

1 - smear show large gram + rods
2 - Culture anaerobically and identify by sugar fermentation
3 - colony exhibit double zone of hemolysis
4 - Culture on Egg yolk

Answer 5

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Question 6

Clostridium difficile

1 - ELISA to detect antibodies to toxins
2 - cytotoxicity tests
3 - PCR to detect toxin gene

Answer 6

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Question 7

Corynebacterium diphtheria

1 - isolate organism and culture in loeffler media and blood culture

2 - antibody - based gel diffusion precipitation test

3 - PCR to detect toxin gene

4 - Gram stain and methylene blue to reveal metachromatic granules

Answer 7

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Question 8

Lesteria monocytogenes

1 - small and gray colonies on culture
2 - organism exhibit norrow zone of hemolysis on blood culture
3 - sugar fermentation test
4 - It’s motile

Answer 8

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