Microbiology cram2 Flashcards
Lactose formers
“CEEK”
Citrobacter
Enterobacter
E.Coli (K1 capsule most important)
Klebsiella
Non lactose formers
“SHYPS” Shigella Yersinia enterolytica (AKA Pestis) Proteus Salmonella
May lack color
“These rascals may microscopically lack color”: Treponema Ricksetta Mycobacterium Mycoplasma Legionella Chlamydia
cAMP
“CAPE” Cholera Anthracis (Poly D glutamate capsule) Pertusis (via Gi) E.coli (LT enterotoxin)
Have Capsules [ie… are Quellung Reaction (+)]
“Some killers have pretty nice capsules”
Strep. Pneumoniae
Klebsiella
HiB
Pseudamona Aeroginosa
Neisseria meningitis
Cryptococcus neoformans (only encapsulated fungal pathogen)
Dimorphic Fungi
“Can Also Have Both Shapes” Cocciodes Aspergillus Histolpasma Blastomyces Sprothrix schenkii
Have b Prophage
“OBED”
O = Salmonella
B = Botulinum
E = Erythrogenic strep
D = Diptheria
Spore Forming Bacteria
Bacilus & Clostridium (have calcium di-picolinate)
IgA Proteases
Neisseria, Haemophilus, S. pneumoniae
Widal Test
Salmonella (Salmonella begins in the ileocecal region) agglutination indicates Abs to O, H, Vi Salmunella Ags
Wayson’s Stain
Yersinia
Pneumonic Plaque Transmission
Person to person cf w/ Bubonic plaque that was via infected flea
Splenectomy
Predisposes to septicemia
Invasins
Yersinia pseudotuberculosis
Fusiform
Vincent’s trench mouth
S. viridans
Dextran mediated adherence
Obligate Aerobes
Pseudomonas & Mycobacterium
Obligate Anaerobes
Clostridium, Actinomyces, Bacteroides
Staph aureus
A Protein, Catalase +/ Coagulase +
Spirochetes
Treponema, Borrelia, Leptospira
Non Motile Gram (+) Rods
Corenybacterium D & Nocardia
Acid Fast Organisms
Mycobacterium; Cryptosporidium; Nocardia (partially); Legionella micdadei; Isospora
Pigment Producing Bacteria
Serratia – red (can cause pseudohemoptysis)
Pseudomonas A – piocyanin blue/green
Staph Aureus – yellow – Protein A
Mycobacteria – photo/scoto chromogenic – caritinoid – yellow/orange
Corneybacterium D – black/gray – pseudomembrane plaque in throat
Bacteroides (Porphyromonas) melaninogenicus – black (heme)
E. coli – irredescent green sheen
Bacterial Morphology
Pneumococci – lancet shaped diplococci
Neisseria – kidney bean shaped diplococci
Camphylobacter – gulls’ wings/comas
Vibrio Cholera – coma shaped
Corneybacterium D – club shaped (nonmotile, G+Rod)
Yersinia – safety pin seen in Wayson’s stain
Inclusion Bodies
Rabies – Negri bodies – intracytoplasmic
Pox virus – Guarnieri – intracytoplasmic & acidophilic
CMV – Owl’s eyes – intracytoplasmic & intranuclear
HSV – Cowdry bodies – intranuclear
Schistosoma Japonicum Monsoni
Intestinal – contact w/ bad water
Schistosoma Haematolium
Vesicular – contact w/ bad water
Non Human Schistosom
Swimmer’s itch – contact w/ bad water
Clonorchichis
Chinese liver fluke – eating raw fish. Txt: Praziquantel
Fasciola Hepatica
Sheep – eating raw fish. Txt: Praziquantel
Fasciola Biski
Giant intestinal flukes – eating raw fish. Txt: Praziquantel
Paragonimus Westermani
Lung fluke – eating raw fish. Txt: Praziquantel
Oxidase (+)
Neiserria and most Gram (-)s
Micro Aerophilic
Camphylobacter & Helicobacter
Urease (+)
All Proteus – can cause Staghorn/Struvite calculi (NH4- Mg2- stones): alkaline urine Ureaplasma Campylobacter pylori (Helicobacter) Cryptococcus Nocardia
Coagulase (+)
Staph A & Yersenia pestis
Obligate Intracellular Bacteria
Chlamydia Pistacci (Chlamydia do not make own ATP); Mycobacterium Leprae; all Rickettsia except Roachalimea (make suficient ATP to survive)
Protozoa
Plasmodium; Toxoplasma ghondi; Babesin; Leishmania; Trypanosoma Cruzi
Obligate Non Intracellular Parasites
Treponema palidum & Pneumocystis Carinii (cannot be cultured on inert media but can be found extra cellularly in the body)
Haemophilus Factors
X = Protoporphyrin & V = NAD
All cocci are
Gram (+) except for Neisseria & Moraxella
“Eaton Fried Eggs”
Mycoplasma pneumoniae has fried egg colonies on Eaton agar (needs cholesterol)
Mycoplasma
No cell wall. Membrane has cholesterol. Smallest living bacteria.
P1 protein inhs ciliary action
Fried egg colonies
Atypical pneumonia – young adults
Sabrands
Fungal media
Malassazia furfur
Spaghetti & meat ball
Measles’ 3C’s
Cough – Coryza – Conjunctivitis. Can also have photophobia
May lead to subacute Sclerosing Panencephalitis
Non Motile Bacilli & Clostridium
B. Anthracis & C. Perfringens
Bloody diarrhea agents
EIEC – EHEC – Shigella - Yersenia enterocolitica – Entaemeba histolytica – Salmonella – Campylobacter jejuni
YW-135CA
N. meningitidis vaccine capsualr polysaccharide strains
Indian Ink
Cryptococcus neoformans
Naegleria causes
Colonization in the nasal passages after swimming
Need Cysyeine for growth
“Ella likes cysteine”: Francisella Brucella Legionella Pasturella
Endotoxins, G(+) or G(-)
Gram (-): N. meningitidis
Ecthyma Gangrenosum, seen w/
Pseudomonas aeroginosa. Target shaped skin lesions w/ a black center and red ring surrounding the lesion
Endospores G(+)
Gram (+): Bacillus & Clostridium – made up of dipicolinate & Keratin
Multi Brain Abscess
Nocardia
Single Brain Abscess
Actinomyces israelli
risk for Strep pneum Infection
Asplenic; Sickle cell anemia; immunocompromising illness
a Hemolysis/Optochin Sensitive
Strep. Pneumoniae
a Hemolysis/Optochin Resistant
Strep. Viridans (Subacute Endocarditis)