Microbiology

Question 1

List 2 plating media would you use to identify and isolate bacterial conjunctivitis:

What kind of stain would you perform?

Answer 1

Plating media:

1. Chocolate agar
2. 5% SBA

Gram Stain

Question 2

List the agent that causes human monocytic ehrlichiosis (HME):

Answer 2

Ehrlichia chaffeensis

Question 3

List the agent that causes Rocky Mountain spotted fever (RMSF):

Answer 3

Rickettsia rickettsii

Question 4

What is the vector for Rocky Mountain spotted fever?

Answer 4

Ticks

Question 5

List the causative agent for Lyme disease:

Answer 5

Borrelia burgdorferi

Question 6

What is the causative agent of genital warts?

Answer 6

HPV - human papillomavirus

Question 7

List the causative agent of Chancroid:

Answer 7

Haemophilus ducreyi

Question 8

List the causative agent of syphilis:

Answer 8

Treponema pallidum

Question 9

Refer to the photo below.

List the disease caused by this organism:

Answer 9

Trichomonas

Question 10

Refer to the photo below:

What organism is causing the white coating on the patient’s tongue?

Answer 10

Candida albicans

Question 11

What is the most common organism that causes nongonoccoal urethritis (NGU)?

Answer 11

Chlamydia trachomatis

Question 12

Which test is the gold standard in diagnosing bacterial meningitis?

Answer 12

CSF culture

Question 13

List any 3 nonviral causes acute meningoencephalitis:

Answer 13

1. L. monocytogenes
2. Ricketttsia
3. Bartonella
4. Mycoplasma
5. B. burgdorferi
6. T. gondii

Question 14

List 2 spirochetes associated with CNS infections:

Answer 14

1. T. pallidum
2. B. burgdorferi

Question 15

List 3 common organisms that cause acute meningitis:

Answer 15

1. N. meningitidis
2. H. influenzae
3. L. monocytogenes

Question 16

Refer to the photo below:

What organism is shown here growing on hektoen enteric agar?

Answer 16

Salmonella spp.

Question 17

List 3 late or slow lactose-fermenting organisms commonly isolated from the GI tract that initially appear colorless and transition to light pink on Maconkey agar:

Answer 17

1. Serratia spp
2. Citrobacter spp
3. Hafnia spp

Question 18

Refer to the photo below:

What is causing the black color of the cultured organism on this XLD agar media?

Answer 18

H₂S production from Salmonella spp.

Question 19

Refer to the photo below:

What organism do you suspect is growing in this XLD agar media?

Answer 19

Shigella spp.

Does not ferment any sugars and appear red or clear on XLD.

Question 20

List media that is highly selective at promoting growth of Vibrio spp.:

Answer 20

TCBS - Thiosulfate-citrate-bile salts-sucrose agar

Question 21

List media that is highly selective at promoting growth of C. difficile:

Answer 21

CCFA - cycloserine-cefoxitin-fructose agar

Question 22

List the organism commonly associated with peptic ulcer disease:

Answer 22

Helicobacter pylori

Question 23

List the gram (+), anaerobic, spore and toxin producing organism which is commonly associated with nosocomial antibiotic-associated diarrhea.

Answer 23

Clostridium difficile

Question 24

Jock itch and ringworm are cause by this organism:

Answer 24

Tinea spp.

Question 25

List 2 acid fast stains used to identify mycobacteria:

Answer 25

1. Ziehl-Neelsen 2. Kinyoun

Question 26

List the causative agent for whooping cough:

Answer 26

## Footnote *Bordatella pertussis*

Question 27

List the condition in which prions are the causative agent.

Answer 27

Kuru or transmissible spongiform encephalopathies (TSE), CJD

Question 28

Individuals with Hepatitis D infection are concurrently infected with an additional virus:

Answer 28

Hepatitis B (HBV)

Question 29

List hepatitis viruses that most likely result in chronic infection:

Answer 29

HBV HCV

Question 30

How is Hepatitis A transmitted?

Answer 30

Fecal - oral route

Question 31

Which viral category is responsible for causing colds?

Answer 31

Rhinoviruses

Question 32

Which viral category is responsible for causing AIDS?

Answer 32

Retroviridae

Question 33

Koplik spots are symptoms associated with which viral disease?

Answer 33

Measles

Question 34

List a common virus implicated in causing croup, bronchitis, and interstitial pneumonia.

Answer 34

RSV - respiratory syncytical virus

Question 35

*Refer to the photo below.* Identify the worm indicated by the arrow:

Answer 35

## Footnote *Strongyloides*

Question 36

*Refer to the photo below:* Identify the type of ova pictured at the arrow:

Answer 36

Hookworm ova

Question 37

*Refer to the photo below:* Identify the type of ova pictured at the arrow:

Answer 37

Ascaris lumbricoides ova

Question 38

*Refer to the photo below:* Identify the type of ova pictured at the arrow:

Answer 38

*Trichuris trichiura* ova

Question 39

*Refer to the photo below:* Identify the type of ova pictured at the arrow:

Answer 39

Paragonimus westermani egg

Question 40

*Refer to the photo below:* Identify the type of ova pictured at the arrow.

Answer 40

*Clonorchis sinesis* ova

Question 41

*Refer to the photo below:* This test is diagnostic for which bacteria spp?

Answer 41

## Footnote *Corynebacterium diphtheriae*

Question 42

*Refer to the photo below:* This test is diagnostic for which bacteria spp?

Answer 42

## Footnote *Listeria*

Question 43

*Refer to the photo below:* This test is diagnostic for which bacteria spp?

Answer 43

Group B β-hemolytic streptococci

Question 44

*Refer to the photo below:* The wagonwheel appearance of the white colonies is characteristic of:

Answer 44

## Footnote *Moraxella spp.*

Question 45

*Refer to the diagram below:* This test is used to identify which bacterium?

Answer 45

## Footnote *H. influenzae*

Question 46

A selective media containing sheep rbcs and phenylethyl alcohol that is used to supress gram (-) bacilli is called:

Answer 46

PEA agar

Question 47

Bacteroides Bile esculin (BBE) agar when cultured and incubated anaerobically will often grow which bacterium?

Answer 47

## Footnote *B. fragilis*

Question 48

JEMBEC plates with a characteristic "Z" growth pattern are used in the identification of the following bacterium:

Answer 48

## Footnote *N. gonorrhoeae*

Question 49

*Refer to the photo of a urease test below:* Which tube is considered positive?

Answer 49

Pink tube

Question 50

Define syncytial:

Answer 50

Multinucleate cells

Question 51

Define median infectious dose (ID50):

Answer 51

Minimum number of organisms required to cause diarrhea in 50% of people exposed.

Question 52

List the E.coli strain responsible for enterohemorrhagic E. coli that often develops into hemolytic uremic syndrome (HUS):

Answer 52

O157:H7