Microbiology Flashcards
Bipolar staining safety pin appearance
Yersinia pestis- plague H ducreyi - chancroid Calymatobacter garnulomatosis Burkholderia mallei B paseudo mallei Vibrio para hemoliticusp
Incubation period Vibrio para haemolytic uss
16 -24 hrs
Chinese letter appearance or cueniform arngmnt
corynebacterium diphtheria
Capsulated organisms
Pneumococcus Antracis bacilus Klebsiella I hemophilus Yersenia species Bordella spe Meningococcclaustridum perforinges Vibrio para hemo
Capsule mostly made up off
Polysaccharides
Except bacillus antacis
Polypeptides
Quellung reaction- capsular swelling seen in
Pneumococcus
Macfadyens reaction done for
Peptide capsule- bacillus antracis
Function of capsule
Anti phagocytic- Prevent opsonisation
Opsonisers
C3b, fc of IGg, IgM
major opsonise
C3b
Names of. Motility
T- tumbling( listeria)
D- darting(v cholera)
S- swarming(protease)
FALLING- leaf like(giardia lamblia) parasite
Stately motility
Slow form of Swarming
Seen in clustridium species
Demonstrate motilityMethod
Hanging drop method
Semi solid agar
Enriched media( always solid)
Ba- sheep blood 5%
Ca
Lss- lofflers serum slope-cd
Enrichment media always liquid
Celetine f broth- salmonella/shigella
Differential indicative sekective
Mac-conky agar
Only for gnb grws here
Contents of mac conky agar
P-peptone l-lactose A-agar N-neutralised T-touroclorate sodium
Media for anaerobes
Rcm - robertson cooked meat medium
Turns black- proteolytic(c tetani)
Turns pink-sacrolytic(c perforinges)
Vibrio cholera transportmedia
Venkat raman ramakrshnan media(V-R) media
Cary blair
Transport media for streptococcus pyogenes
Pikes media
Transport media for gonococci and meningococci
Stuarts and amine media
Transport media for shigella nd salmonella
Sachs buffer glycerol saline
Mostly medically important bacteria belongs to
Mesophite(20-40c)
Antibiotic Sensitivity testing(ast)
Kirby bauer d this diffusion test
Done on- muller hilton agar
Violet color grape like cluster seen in
Staph aureus
Streptococcus pyogenes cx media
CVBA- crystal violet blood agar
Normal commensal of birth canal
Gbs s agalactiae
Enhanced or butterfly zone hemolysis seen in
Gbs grp b stretoco
S agalactiae
Cx media for enterococcus
Bile aesculin agar
Produces - BLACK COLORED COLONIES
Normal commensal of gut
Enterococcus
Doc for enterococcus
Panicillin+aminoglycoside
Lanceolate shape (flame)
Draughtsman like appearence
Carrom coin appeearence seen in?
Pneumococcus
Mcc of meningitis in adults
Pneumococcus
Doc for pneumococcus
Penicillin
Kdney shape seen in
Gonococci
Lens shape seen in
Meningococci
Rash + petechial lesion seen in which meningitis
Meningococcal meningitis
Meningococci cx on
THAYER MARTIN MEDIA
Most dreadful complication of meningococci
WATER HOUSE FRIEDERHEIN SYNDROME
Vaccine use for meningococci
MCV-4 ( meningococcal conjugate vaccine
Ophtalmia neonatorum
Mcc of chlamydia trechomatous
Also gonococci
Gonococci causing diseases
Males- urethritis
Female- cervicitis
Doc for gonococci
Ceftriaxone
3rd generation cephalosporin
C dihptheria cx media
Lss loflers serum slope
Best cx media: pottassium telurate agar - definitive dx can be made
Cx media of c diphtheria
H- hoyles
M macleoids
T tinsdale
Diphtheria is?
Toxemia not bacterimia
Bull neck seen jn
C diphtheria
C diphtheria lab dx
Eleck gel
Shick test
Pcr
Elisa
Erythrasama caused by
Coryne bacterium minnutissimum
Bacillus antracis appearence
M medusa head appearnce
I Inverted fir tree app
S string of pearl app
BAMBOO bambo stick app
Cx media for b antracis
P PROTECTIVE FACTOR
L LETHAL FACTOR
E EDEMA FACTOR
T [TRIPARTINE TOXIN]
Bacillus cereus cx media
MYPA- mannitol yolk polymyxin b agar