Microbiology

Question 1

What is the CD receptor on B cells where EBV infections?

Answer 1

CD21

C3d R

Question 2

Where are the 2 locations of epithelial cells that are infected by EBV?

Answer 2

Oro and nasopahrynx

Question 3

Which cells does EBV reside in for latent EBV?

Answer 3

Memory B cells

Question 4

After the EBV virus binds to the C3d receptor, what happens to the B cells?

Answer 4

It grows

Question 5

Which proteins do B cells express after being stimualted to grow? (4)

Answer 5

EBNAs
Latent Proteins
LMPs 1 and 2
EBER molecules

Question 6

Which B cell proteins are DNA binding protiens that are essential for establishing an maintaining the infection?

Answer 6

EBNA

LPs

Question 7

Which protein from the B cells is a membrane protein with oncogene-like activities?

Answer 7

LMPs

Question 8

This is the B Cell protein that is transcribed and translated during early genes of the virus and the lytic cycle.

Answer 8

ZEBRA transcriptional activator protein

Question 9

What is the viral attachement protein that promote fusion of the envelope with the cell membrane, which occurs after the synthesis of DNA polymerase and replication of DNA?

Answer 9

GP350/220

Question 10

When do you see the anti EBNA marker?

Answer 10

After resolution of infection

Question 11

When do you see the early Ag?

Answer 11

After an infected cell has entered lytic cycle

Question 12

What are the 2 components of the late VCA protein, which is found in virus-producing cells?

Answer 12

Anti-VCA IgM = transient

Anti-VCA IgG = persistent

Question 13

When do you see the membrane Ag?

Answer 13

same as VCA - late protein, in virus producing cells

Question 14

When do you see the Heterophile Ab?

Answer 14

Early, where EBV induces B cell proliferation

Question 15

An overactive immune response (from T cell) to EBV infection can produce what problem?

Answer 15

Infectious mononucleosis (IM)

Question 16

So what are the 2 problems if there is lack of effecting immune control of EBV infections?

Answer 16

Lymphoproliferative disease

Hairy cell leukoplakia

Question 17

What is the transmission method for EBV?

Answer 17

Slaiva (kiss kiss)

:-*

Question 18

These are the atypical lymphocytes made when Tc cells controls B-cell proliferation, induced by EBV.

Answer 18

Downey cells

Question 19

Is EBV heteophile + or -?

Answer 19

+

Question 20

The B cell activation and proliferation causes a false production of IgM to what Ag, which screens for heterophiles?

Answer 20

Paul-Bunnell Ag

Question 21

True or False: a good way of distinguising EBV from CMV infections is to see if there are Downey cells, as they’e only present in EBV infecitons.

Answer 21

FALSE

Theyre in both, but mostly in EBV infections.

Question 22

This is there condition where there is heterophile +, Sx similar to strep pharyngitis (gray/green throat exudate), extreme fatigue, lympadenopathy, exydate pharyngitis, splenomegaly and splenic rupture.

Answer 22

Infectious mononucleosis

Question 23

Treatment of IM with what drug can cause an erythematous rask because of an allergic rxn?

Answer 23

Ampicillin

Question 24

What are the 2 main complicaitons to IM?

Answer 24

Splenic rupture

Neurological complciations

Question 25

What age group is at most risk for IM?

Answer 25

Teenagers

Question 26

Which pts are at highest risk for life-threatening neoplastic disease in IM?

Answer 26

Immunocompromised pts

Question 27

This is the stage of IM where you see heterophile Ab present, mono symptoms, no EBNA, VCA IgM/IgG present, and possible EA.

Answer 27

Early IM

Question 28

Which of the following markers are present during the Chronic form of IM? Heterophile Ab, VCA IgM or EBNA, VCA IgG, EA

Answer 28

VCA IgG and EA

Question 29

Which of the following markers are present during the latent form of IM? Heterophile Ab, VCA IgM, VCA IgA, EBNA, VCA IgG, EA

Answer 29

VCA-IgA EA EBNA**

Question 30

Which of the following markers are present during the PAST INFECTION form of IM? Heterophile Ab, VCA IgM, VCA IgA, EBNA, VCA IgG, EA

Answer 30

VCA-IgA EBNA (no EA)

Question 31

This is the condition where there is white crap on the side of the tongue from EBV infections in AIDS pts.

Answer 31

Hairy Oral Leukoplakia

Question 32

Where is candida thrush located, which makes it distinguishable from hairy oral leukoplakia?

Answer 32

Thursh is at the back of the throat

Question 33

What does EBV do to B cells in lymphoproliferative disease?

Answer 33

Stimulates and immoralizes them

Question 34

This is the monoclonal B-cell lymphoma of the jaw and face from chromsosomal transplaction of the cMYC oncogene to have a very active promoter.

Answer 34

Burkitts lymphoma

Question 35

In burkitts lymphoma, who is at risk?

Answer 35

African kids

Question 36

What co-infection must be present in Burkitts lymphoma?

Answer 36

malaria

Question 37

True or False: Burkitts lymphoma is curable with steroids.

Answer 37

True

Question 38

What do people lack to cause B cell lymphoma, instead of IM?

Answer 38

T-cell immunity

Question 39

This is the lymphoproliferative disease that is endemic in adults in Asia, contains EBV DNA, and is of epithelial origin.

Answer 39

Nasopharyngeal carcinoma

Question 40

Instead of getting IM, what is the lymphoproliferative disease that transplant recipients can acquire?

Answer 40

Post transplant lymphoproliferative disease

Question 41

This is the condition where therea re congenital deficiencies of T-cell fxn (SLAM), where the T cell can control B-cell growth during normal immune response to Ag or because of EBV.

Answer 41

X-linked lymphoproliferative disease

Question 42

What is the main site of latency for CMV?

Answer 42

Mononuclear lymphocytes

Question 43

How do u get CMV?

Answer 43

Blood, tissue, and most body secretions

Question 44

What does CMV do to block the CD8 and CD4 recognition of cells?

Answer 44

Downregulating MHC-I

Question 45

What cell type does CMV block from killing the cell by fooling that it does have an MHC-I?

Answer 45

NK cell

Question 46

True or False: CMV is the most common viral cause of congenital defects.

Answer 46

True

Question 47

What is the 2 ways neonates can get CMV?

Answer 47

Transplacental | Viral ascent from cervix

Question 48

How does a baby/child get CMV? think secretions.

Answer 48

Boob milk, saliva, tears, and urine

Question 49

What are the 3 ways adults can get CMV infections?

Answer 49

STD, blood transfusions, organ graft

Question 50

What are the manifestations of congenital infection of the neonate?

Answer 50

``` Low birth weight Thrombocytopenia Intercerebral calification Microcephaly Jaundice Hepatosplenomegaly Hearing loss MR ```

Question 51

What food does a baby look liek when there is cytomegalic includions all over the body from CMV infections?

Answer 51

A blueberry muffin

Question 52

This is the condition where there is heterophile negative mono, possible Downey cells, and signs of hepatitis.

Answer 52

Mononucleosis | from CMV

Question 53

What is the #1 problem of CMV infections in IC pts?

Answer 53

Retinitis

Question 54

True or False: CMV is the most responsible for kidney transplant failures in organ transplants.

Answer 54

True

Question 55

So which mononucleosis is heterophile +, EBV or CMV?

Answer 55

EBV

Question 56

This is the sign on CMV where there is an enlarged cell with basophilic intranuclear includion bodies.

Answer 56

Owl's eye inclusion

Question 57

What are the 2 tests u can do to screen for the CMV viral Ag for Dx?

Answer 57

IF or ELISA

Question 58

What is the Tx for severe CMV infection in IC pts?

Answer 58

Ganciclovir

Question 59

What other drugs can u give for CMV infections?

Answer 59

Valganciclovir Cidofovir Foscarnet

Question 60

This is an opportunistic infection in AIDS pts, where a virus infects a limited # of endothelial cells, monocytes, eithelial and sensory nerve cells, and causes neoplasms.

Answer 60

Kaposi sarcoma | HHV-8

Question 61

What is the rare type of B-cell lymphoma from HHV-8 infections?

Answer 61

Primary effusion lymphoma