MicroBiology Flashcards

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1
Q
A

B is Correct.

Any viruses that had already completed the replication process when the cyanide was added will not be affected, and will remain infectious.

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2
Q

If the ratio of adenine to thymine in a DNA virus is not one to one, what can be said about the genome of this virus?

A

Adenine base pairs with thymine in double-stranded DNA. Thus, for every A there should be one T for a one to one ratio of A to T. If the ratio differs from this, the genome must be single-stranded DNA, or RNA, which has no T.

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3
Q

A disease agent that is isolated from a human cannot reproduce on its own in cell-free broth but can reproduce in a culture of human cells. In its pure form it possesses both RNA and DNA. Is it possible that the disease agent is a virus?

A

No, it can’t be a virus. Viruses possess only one kind of nucleic acid. The disease agent is another kind of obligate intracellular parasite.

NOTE: certain bacteria like Chlamydia, can only reproduce inside host cells.

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4
Q

What is the likely result if a viral genome is tripled in size?

A

The viral genome will probably no longer fit within the normal viral structure, and the genome will therefore not be packaged into infectious viral particles.

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5
Q

How do ribosomes used to translate viral proteins compare to host ribosomes?

A

Viruses use host ribosomes. Viral and host ribosomes are translated by the same ribosomes.

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6
Q
A

C is Correct.

The only way to do this is with overlapping several frames.

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7
Q

Why might a bacteriophage inject its DNA, while animal viruses don’t?

A

a bacteriophage must puncture the cell wall, while animal viruses can be internalized whole into animal cells since animals don’t have cell walls.

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8
Q

All phages and plant viruses are naked viruses, meaning they don’t have envelopes. Why is this?

A

Viruses acquire envelopes by budding through host membranes. Phages and plant viruses infect hosts that have cell walls. When these viruses begin to exit the cell, the cell wall is destroyed and the host membrane is ruptured. Thus there is no membrane through which the remaining viruses must bud; they simply escape in lytic explosion.

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9
Q

If antibodies to a viral capsid protein are ineffective in blocking infection, what might this indicate about the virus?

A

It suggests that the virus is enveloped, so the antibody can’t reach its epitope on the capsid surface in the infectious virus.

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10
Q

If lysozyme of a bacteriophage were an early gene, would this be advantageous to the virus?

A

No. The host would lyse before the phage had time to replicate and assemble.

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11
Q
A

The intial decrease is due to the simple fact that many phage have injected their genomoes into hosts and are no longer infectious.

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12
Q
A

The 35S cysteine will be incorporated into viral coat proteins and the 32P phosphate will be incorporated into the viral nucleic acid genome in newly released viral particles because proteins contain no P and nucleic acids contain no S.

When these viruses infect bacteria, their nucleic acids are injected into bacteria while the capsid proteins remain on the exterior, which means only 32P will be found in the interior of the newly infected cells.

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13
Q
A

A is correct.

When two viruses infection the same cell it is called co-infection. Some normal viruses will result and some genomes from defective viruses will get packaged into capsids made from proteins encoded by the normal virus. The latter will be capable of infectiong new hosts, but when they do their progeny will not survive due to the capsid abnormality.

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14
Q

Why would a bacterial gene, carried by a virus integrated with viral genes into a new bacterial genome, not be repressed along with the viral genes during lysogeny?

A

Prophage latency results from a viral repressor protein binding to viral DNA in a sequence-specific manner. The specific DNA sequence to which the repressor binds is present in the viral genes but not in the bacterial genes, so the bacterial genes can be expressed while the viral genes are repressed.

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15
Q
A

Yes.

The soluble CD4 protein would bind to the virus’s CD4 receptor and block attachement of the virus to T cells.

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16
Q
A
  1. the receptor has specific role in the normal physiology of the host, which a mutation might ocmpromise.
  2. Viruses generally evolve so rapidlly that they can keep up with any changes in the host, but this is not an absolute rule. Cells of our immune system keep us alive by keeping up with most microorganism’s tricks.
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17
Q
A

A is correct.

Some envelope proteins are encoded by the virus and some are derived from the host’s membranes during budding.

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18
Q

True or False?

+ RNA viruses must encode RNA-dependent RNA polymerase and have to carry it.

A

False. They do have to encode RNA-dependent RNA polymerase but they don’t have to carry it.

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19
Q

In cellular respiration, what acts as the final electron acceptor if oxygen is not available?

A. Glucose

B. NADH

C. Pyruvate

D. FADH2

A

C. Pyruvate

20
Q

What is the role of RNA-dependent RNA polymerase?

A

to copy the RNA genome for viral replication; the host never makes RNA from RNA.

21
Q

Will an infectious virus be produced if the genome of an enveloped (+) strand RNA virus is added to an extract prepared from the cytoplasm of eukaryotic cells that retains translational activity but lacks DNA replication or transcription of host genes?

A

No. (+) strand RNA virus will be able to produce viral genome and proteins, but progeny will not be able to acquire the envelope they need to be infectious.

22
Q

If a viral genome is (+) strand RNA, what is used as a template by the RNA dependent RNA polymerase?

A

The virus needs the complementary strand as a template: the (-) strand RNA. Thus the RNA dependent RNA polymerase produces a (-) strand intermediate before generating new (+) strand genomes.

23
Q

True or False?

(-) RNA viruses must encode AND carry RNA-dependent RNA polymerase.

A

True

24
Q

How does ketogenesis trigger energy production?

A. Acetyl-CoA is produced for use in the Krebs cycle

B. Acetone is produced for use in the PDC

C. β-hydroxybutyrate is produced for use in glycolysis

D. Acetoacetate is produced for use in the electron transport chain

A

A. Acetyl-CoA is produced for use in the Krebs cycle

25
Q

What are the two phases of the pentose phosphate pathway?

A. Oxidative and non-oxidative

B. Non-oxidative and reductive

C. Deaminating and hydrolytic

D. Reductive and deaminating

A

A. Oxidative and non-oxidative

26
Q

In fatty acid metabolism, how many carbons are removed per round of β-oxidation?

A. 8

B. 2

C. 3

D. 4

A

B. 2

27
Q

Which of the following intermediates in gluconeogenesis is NOT also an intermediate of glycolysis?

A. Glucose-6-phosphate

B. Fructose-1,6-bis-phosphate

C. Oxaloacetate

D. Phosphoenolpyruvate

A

C. Oxaloacetate

28
Q
A
29
Q

How many net ATPs are produced when a prokaryotic cell fully oxidizes one molecule of glucose?

A. 36 ATP

B. 32 ATP

C. 38 ATP

D. 30 ATP

A

B. 32 ATP

30
Q

Do (-) strand RNA viruses use host enzymes to cataylze RNA production in transcription or in replication of the genome?

A

Neither.

Viral RNA-dependent RNA polymerase first makes (+) strand as mRNA and then uses the (+) strand as the template to replicate new (-) strand genomes.

31
Q

Retroviruses arre theoretically not required to carry RNA-dependent DNA polymerase for reverse transcription, they need only encode it. Why?

A

Because the viral RNA genome can be translated by host ribosomes; thus reverse transcriptase may be made after the viral genome enters the host.

32
Q

Given the limited information that viruses may contain in their genomes, why do double-strandedd DNA viruses carry around genes for an enzyme possessed by the host?

A

The host cell will only make dNTPs in prepraration for replication. If the virus wants to reproduce without waiting for the host to do so, it must encode its oen enzymes for the synthesis of DNA building blocks.

33
Q

After intergration of retroviruses into the cellualr genome, a reverse transcriptase inhibitor is added in the cell. Will the production of new viruses be blocked?

A

No. Reverese transcriptase is required for only one phase of the retrovirus life cycle: the copying of the viral RNA genome into DNa so that it can intergrate into the host genome and be transcribed.

Once the viral genome is integrated, transcription to produce viral mRNA and new viral RNA genomes doesn’t involve reverse transcriptase. It can proceed with the normal host-cell enzymes.

34
Q

Why don’t RNA viruses carry genes possessed by the host like double-stranded DNA viruses do?

A

Trancription is always accuring in all cells so NTPs are always present.

35
Q

What is a factor likely to limit the size of RNA genomes?

A

The error rate in RNA synthesis is much higher than with DNA because of the mechanisms to proofread and correct errors aren’t present in RNA synthesis like they are in DNA synthesis.

If an RNA genome were too large, every copy of the viral genome sythnesized would suffer from so many errors that no infectious virus could be produced.

36
Q

Some DNA viruses induce infected host cells to enter mitosis and may even overrride cellular inhibition of cell division so strongly that the cell becomes cancerous; what is the advantage to the virus inducing host-cell division?

A

To replicate, the DNA virus must either provide all the necessary components itself, infect a cel that is already dividing, or induce the cell it infects to enter mitosis and produce ingredients for DNA synthesis.

37
Q

If two different adenoviruses infect the same cell, one with a deleted E1A gene and another with a deleted NDA polymerase gene, will successful infection of the cell occur?

A

Yes due to complementation.

The mutant viruses will complement each other, one providing the missing E1A protein and the other providing DNA polymerase. These are called helper viruses.

38
Q

How do prions violate the Central Dogma?

A

Prions are self-replicating, taking transcription and translation of the process. Proteins are shaped based on other proteins.

39
Q

What is the central dogma?

A

The central Dogma states that information flows in the nucleiotide form from DNA to RNA (transcription), and then in its amino acid form from RNA to protein (translation).

40
Q

What shape are bacilli?

A

rod shaped

41
Q

Cocci bacteria are _____shaped.

A

round

42
Q

Spirochetes and spirilla bacteria are ______shaped

A

spiral

43
Q

Would a protoplast moved from salt water to fresh water shrivel or burst?

A

It would burst since water ould flow into the cell by osmosis.

44
Q

Which bacteria would be more susceptible to lysis when treated with lysozyme: Gram negative or Gram positive?

A

Gram-positive.

Lysozyme hydrolyzes linkages in peptidoglycan to weaken the cell wall. The peptidoglycan in gram-positive walls is more accessible, since these cells no not posssess an additional outer layer like gram negative bacteria.

45
Q

Can viruses move via flagellar propulsion to find host cells?

A

No. viruses lack any means of energy production on their own and any means of active movement. They rely on diffusion.

46
Q
A

C. Chemoheterotroph

47
Q
A

D. Photoheterotroph