Brainscape's Knowledge GenomeTM


Top Flashcards (Certified)
All Flashcards

Bio 2A > Microbiological Tools > Flashcards

Microbiological Tools Flashcards

1
Q

most commonly used in handling (isolating, transferring, and cultivating) microbes

A

inoculating loop / wire loop

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
2
Q

inoculating loop movement

A

finger movement

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
3
Q

used for stabbing bacteria vertically into deep tube of media to determine the shape of the hole digested by the bacteria

A

inoculating needle

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
4
Q

inoculating needle movement

A

stab movement

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
5
Q

l shape glass rods

A

use for spreading the bacteria in the plate

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
6
Q

can be used as spreader
can be used during sampling instead of inoculating loops

A

cotton swab

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
7
Q

allow users to extract or deliver small amount of liquid or microbial dilutions

A

mechanical pipettes

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
8
Q

unit of mechanical pipettes

A

uL

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
9
Q

20 uL

A

yellow tip

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
10
Q

blue tip

A

1000 uL

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
11
Q

10 uL
used for extracting DNA

A

white tip

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
12
Q

used in heating substances and in incinerating the specialized tool to avoid contaminants

A

alcohol lamp

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
13
Q

sterilization angle

A

45*

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
14
Q

refers to various types of glass instruments and containers used for conducting experiments, handling chemicals, and cultivating microorganisms.

A

laboratory glasswares

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
15
Q

used for the cultivation of microorganisms on solid media

A

petri plates

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
16
Q

refers to the technique of spreading a small volume of microbial sample (usually a bacterial culture) onto the surface of a solid agar medium in a systematic pattern

A

streaking

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
17
Q

can be used for culturing, maintaining and storing cultures specifically in broth

important in microbial dilutions for microbial count

A

test tubes

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
18
Q

position of agar in a test tube

A

slanted

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
19
Q

hold the microbial cultures and store liquids

can also be used for the preparation of culture media

A

erlenmeyer flasks

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
20
Q

hold and work in mixing culture media and other liquids

not recommended in culturing bacteria due to its open space or wide mouth

A

beaker

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
21
Q

a device that uses steam to sterilize equipment and other objects. this means that all bacteria, viruses, fungi and spores are inactivated

A

autoclave

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
22
Q

water requirement of autoclave

A

3 - 6 L

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
23
Q

magsstart ang sterilization page nareach ang ____ for _____

A

121*C, 15mins

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
24
Q

autoclave maintains pressure of

A

15 psi

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
25
a device used to grow and maintain microbiological cultures or cell cultures maintain optimal temperature, humidity and other conditions such as the carbon dioxide and oxygen content of the atmosphere inside
incubator
26
bacteria tend to thrive at
37*C
27
can safely be used in microbiology laboratory for preparation of media the media is convenient , reliable, economical and reproducible, it saves time and the quality of media is superior as compared to media prepared by conventional autoclaving method
microwave
28
laminar flowhood is also called
biosafety cabinet
29
level ng biosafety cabinet sa bsu
level 2
30
provide a high degree of localized control for critical processes the work are is continuously bathed with positive pressure HEPA filtered air to protect the product from contamination designed for the handling of material in a sterile working environment
laminar flow hood
31
it may be used to preserve certain chemical reagents for media it may be used to grow certain types of bacteria this can also be used to preserve cultures of bacteria, yeast, and other microbes
refrigerator
32
macroscopically visible collection of millions of bacteria originating from a single bacterial cell
colony
33
earliest solid medium
cooked cut potato by robert koch
34
is a type of red seaweed that is significant in microbiology due to its use in the production of agar, a substance widely employed as a solidifying agent in culture media.
Gelidium spp.
35
agar percentage in solid media
2%
36
not satisfactory due to low melting point
gelatin
37
gelatin liquify at
24*C
38
who introduced agar as a media
frau hesse / angelina hesse
39
polyssacharide extract obtained from seaweed
agar
40
agar is ideal solidifying agent is bacteriologically
inert
41
inert
no influence on bacterial growth
42
agar is ideal solidifying agent bc it remains solid at
37*C
43
agar is ideal solidifying agent bc it is
transparent
44
media classificiation
according to composition, consistency, selectivity
45
exact composition is not known ex. milk, urine, diluted blood, vegetable juices, meat extracts, infusions and peptone
empirical or natura
46
have known quantities of all ingredients for microorganisms, it provides trace elements and vitamins required by the microbe and especially a defined carbon and nitrogen source
synthetic or defines
47
often used as carbon sources
glucose or glycerol
48
used as inorganic nitrogen sources
ammonium salts or nitrates
49
used for profuse growth mixed organism can’t be seperated
liquid (broth)
50
bacteria may be identified by studying the colony character mixed bacteria can be separated
solid (slant, plate)
51
contains 0.5% agar usually for motility media or isolation of bacteriophage
semi solid (soft agar)
52
refers to a state of cloudiness or haziness in a liquid caused by the presence of suspended particles.
turbid
53
may be used for growth (culture) of bacteria that do not need enrichment of the media
general or non selective
54
example of general or non selective
enterobacteriaceae and Staphylococcus spp.
55
example of non selective medium
nutrient agar
56
media favors the growth of a particular bacterium by inhibiting the growth of undesired and allowing growth of desirable bacteria
selective
57
inhibits the growth of positive gram bacteria selects gram negative bacterium esp. those that can ferment lactose is also diffrential - lactose become pink, non lactose become colorless
MacConkey agar
58
selects the growth of halophiles is also differential - staphylococcus produce yellow colonies bc they ferment mannitol
mannitol salt agar
59
used to distinguish one microorganism type from another growing on the same media uses the biochemical characteristics of a microorganism growing in the presence of specific nutrients or indicators added to the medium to visibly indicate the defining characteristics of a microorganism
differential
60
Staphylococcus species that do not ferment mannitol
Staphylococcus epidermidis
61
Staphylococcus species that ferment mannitol
Staphylococcus aureus
62
Salmonella spp. produce black colonies on this medium is selective and differentiatal
Xylose Lysine Deoxycholate agar
63
color of Escherichia coli on XLD agar
yellow
64
units used in microbial world / microscopy
10^6 = um 10^9 = nm
65
contain only one magnifying lens believed to have a maximum magnifying power of abt 300-
simple microscope
66
produces metallic green sheen in presence of lactose selective of negative gram species is selective and also differential
Eosin Methylene Blue agar
67
total magnification is
objective lens x ocular lens
68
size unit of bacteria
micrometer
69
size unit of virus
nanometer
70
favors the growth of Salmonella spp. and Shigella spp., those that can produce hydrogen sulfide
XLD agar
71
in xld agar, lactose fermenters produce
yellow colonies
72
in xld agar, non lactose fermenters produce
red/pink colonies

Bio 2A (4 decks)

Brainscape helps you reach your goals faster, through stronger study habits.
© 2025 Bold Learning Solutions. Terms and Conditions