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Bio 2A > Microbiological Tools > Flashcards

Microbiological Tools Flashcards

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Biology 101 flashcards
1
Q

most commonly used in handling (isolating, transferring, and cultivating) microbes

A

inoculating loop / wire loop

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2
Q

inoculating loop movement

A

finger movement

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3
Q

used for stabbing bacteria vertically into deep tube of media to determine the shape of the hole digested by the bacteria

A

inoculating needle

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4
Q

inoculating needle movement

A

stab movement

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5
Q

l shape glass rods

A

use for spreading the bacteria in the plate

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6
Q

can be used as spreader
can be used during sampling instead of inoculating loops

A

cotton swab

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7
Q

allow users to extract or deliver small amount of liquid or microbial dilutions

A

mechanical pipettes

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8
Q

unit of mechanical pipettes

A

uL

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9
Q

20 uL

A

yellow tip

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10
Q

blue tip

A

1000 uL

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11
Q

10 uL
used for extracting DNA

A

white tip

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12
Q

used in heating substances and in incinerating the specialized tool to avoid contaminants

A

alcohol lamp

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13
Q

sterilization angle

A

45*

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14
Q

refers to various types of glass instruments and containers used for conducting experiments, handling chemicals, and cultivating microorganisms.

A

laboratory glasswares

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15
Q

used for the cultivation of microorganisms on solid media

A

petri plates

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16
Q

refers to the technique of spreading a small volume of microbial sample (usually a bacterial culture) onto the surface of a solid agar medium in a systematic pattern

A

streaking

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17
Q

can be used for culturing, maintaining and storing cultures specifically in broth

important in microbial dilutions for microbial count

A

test tubes

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18
Q

position of agar in a test tube

A

slanted

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19
Q

hold the microbial cultures and store liquids

can also be used for the preparation of culture media

A

erlenmeyer flasks

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20
Q

hold and work in mixing culture media and other liquids

not recommended in culturing bacteria due to its open space or wide mouth

A

beaker

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21
Q

a device that uses steam to sterilize equipment and other objects. this means that all bacteria, viruses, fungi and spores are inactivated

A

autoclave

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22
Q

water requirement of autoclave

A

3 - 6 L

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23
Q

magsstart ang sterilization page nareach ang ____ for _____

A

121*C, 15mins

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24
Q

autoclave maintains pressure of

A

15 psi

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25
Q

a device used to grow and maintain microbiological cultures or cell cultures

maintain optimal temperature, humidity and other conditions such as the carbon dioxide and oxygen content of the atmosphere inside

A

incubator

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26
Q

bacteria tend to thrive at

A

37*C

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27
Q

can safely be used in microbiology laboratory for preparation of media

the media is convenient , reliable, economical and reproducible, it saves time and the quality of media is superior as compared to media prepared by conventional autoclaving method

A

microwave

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28
Q

laminar flowhood is also called

A

biosafety cabinet

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29
Q

level ng biosafety cabinet sa bsu

A

level 2

30
Q

provide a high degree of localized control for critical processes

the work are is continuously bathed with positive pressure HEPA filtered air to protect the product from contamination

designed for the handling of material in a sterile working environment

A

laminar flow hood

31
Q

it may be used to preserve certain chemical reagents for media

it may be used to grow certain types of bacteria

this can also be used to preserve cultures of bacteria, yeast, and other microbes

A

refrigerator

32
Q

macroscopically visible collection of millions of bacteria originating from a single bacterial cell

A

colony

33
Q

earliest solid medium

A

cooked cut potato by robert koch

34
Q

is a type of red seaweed that is significant in microbiology due to its use in the production of agar, a substance widely employed as a solidifying agent in culture media.

A

Gelidium spp.

35
Q

agar percentage in solid media

A

2%

36
Q

not satisfactory due to low melting point

A

gelatin

37
Q

gelatin liquify at

A

24*C

38
Q

who introduced agar as a media

A

frau hesse / angelina hesse

39
Q

polyssacharide extract obtained from seaweed

A

agar

40
Q

agar is ideal solidifying agent is bacteriologically

A

inert

41
Q

inert

A

no influence on bacterial growth

42
Q

agar is ideal solidifying agent bc it remains solid at

A

37*C

43
Q

agar is ideal solidifying agent bc it is

A

transparent

44
Q

media classificiation

A

according to composition, consistency, selectivity

45
Q

exact composition is not known

ex. milk, urine, diluted blood, vegetable juices, meat extracts, infusions and peptone

A

empirical or natura

46
Q

have known quantities of all ingredients

for microorganisms, it provides trace elements and vitamins required by the microbe and especially a defined carbon and nitrogen source

A

synthetic or defines

47
Q

often used as carbon sources

A

glucose or glycerol

48
Q

used as inorganic nitrogen sources

A

ammonium salts or nitrates

49
Q

used for profuse growth

mixed organism can’t be seperated

A

liquid (broth)

50
Q

bacteria may be identified by studying the colony character

mixed bacteria can be separated

A

solid (slant, plate)

51
Q

contains 0.5% agar

usually for motility media or isolation of bacteriophage

A

semi solid (soft agar)

52
Q

refers to a state of cloudiness or haziness in a liquid caused by the presence of suspended particles.

A

turbid

53
Q

may be used for growth (culture) of bacteria that do not need enrichment of the media

A

general or non selective

54
Q

example of general or non selective

A

enterobacteriaceae and Staphylococcus spp.

55
Q

example of non selective medium

A

nutrient agar

56
Q

media favors the growth of a particular bacterium by inhibiting the growth of undesired and allowing growth of desirable bacteria

A

selective

57
Q

inhibits the growth of positive gram bacteria

selects gram negative bacterium esp. those that can ferment lactose

is also diffrential - lactose become pink, non lactose become colorless

A

MacConkey agar

58
Q

selects the growth of halophiles

is also differential - staphylococcus produce yellow colonies bc they ferment mannitol

A

mannitol salt agar

59
Q

used to distinguish one microorganism type from another growing on the same media

uses the biochemical characteristics of a microorganism growing in the presence of specific nutrients or indicators added to the medium to visibly indicate the defining characteristics of a microorganism

A

differential

60
Q

Staphylococcus species that do not ferment mannitol

A

Staphylococcus epidermidis

61
Q

Staphylococcus species that ferment mannitol

A

Staphylococcus aureus

62
Q

Salmonella spp. produce black colonies on this medium

is selective and differentiatal

A

Xylose Lysine Deoxycholate agar

63
Q

color of Escherichia coli on XLD agar

A

yellow

64
Q

units used in microbial world / microscopy

A

10^6 = um
10^9 = nm

65
Q

contain only one magnifying lens

believed to have a maximum magnifying power of abt 300-

A

simple microscope

66
Q

produces metallic green sheen in presence of lactose

selective of negative gram species

is selective and also differential

A

Eosin Methylene Blue agar

67
Q

total magnification is

A

objective lens x ocular lens

68
Q

size unit of bacteria

A

micrometer

69
Q

size unit of virus

A

nanometer

70
Q

favors the growth of Salmonella spp. and Shigella spp., those that can produce hydrogen sulfide

A

XLD agar

71
Q

in xld agar, lactose fermenters produce

A

yellow colonies

72
Q

in xld agar, non lactose fermenters produce

A

red/pink colonies

Bio 2A (4 decks)

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