microbio - first 50 Flashcards
Basitracin (+) control
*organism
S. pyogenes
Acetamide test
*time and temperature
35’C 7 days
MUG (-)
*result
No blue fluorescence
P. fluorescens
*disease
Bacteremia
Has DNA and RNA
Bacteria
Blood collection
Before the height of fever
Rickettsia stain
Gimenez, Macchiavelo
Anton agent
Listeria
XLD yellow
*organism
E. coli
Lockjaw
*organism
C. tetani
Test to determine C. tetani
Morphology
Malonate (-)
*result
Green
AF rod in nasal mucosa
*diagnosis
Not diagnostic
pH culture media for bacteria
pH 7.2 to 7.4
Diptheria toxin in vitro
test
Elek test
Lowest concentration of drug to kill organisms
*test
MBC or MLC
Test to differentiate S. pyogenes and S. pneumoniae from other
Hemolysis test
Listeria VS. Corynebacteria
*test
Esculin hydrolysis
Penicillin resistant
Beta lactamase
Endospore stain
Schaeffer fulton
Diplococcic ear infection
*organism
Moraxella / Branhamella
Arthrobacter
*organism
Brevibacterium
C. pseudotuberculosis, C. ulcerans
C. diptheriae
Unique in cell wall of AFB
Mycolic acid
Skin, hair, nail fungal infection
Dermatophytoses
Filamentous bacteria, antibiotics
Streptomyces
Curved bacteria with darting motility
Campylobacter
Effect of too much moisture in disk diffusion in growth in AST
Smaller zone growth
SS agar: colorless colonies with black centers
*organism
Salmonella
SS agar: colorless colonies without black centers
*organism
Shigella
Best for Salmonella culture
*test
SS, XLD, Tetrathionate
Sterilization in cans kills endospore
*organism
C. botulinum, E. coli
Cause of floppy baby
C. botulinum
Milk transmitted bacteria
Clostridia, Salmonella
Capnophile requires
5-10% CO2
TRIC there is
Trachoma, Inclusion Conjunctivitis
Source of OX2
P. vulgaris
H2O2 concentration in disinfectants
3% H2O2
Urine GS/CS for ambulatory patient
Random
Urine GS/CS for bedridden
Catheterization
Urine GS/CS for women
Midtream
S. pyogenes is suspected, what test will you do next?
Catalase
E. coli VS. Enterobacter
*IMViC
++– E. coli
–++ Enterobacter
Specimen for TB microscopy
early morning, 3 days
Alternative if there is no clorox in lab
Vinegar
Mycoplasma incubation
Aerobically, CO2
See the cellular details that are microscopically um part
Resolution
Rapid detection in Bacteriology
Decreasing substrate, increasing organism, use of conventional unique substrate
Strain of E. coli that cause diarrheal outbreak in infants
EPEC (O111)
Rule out in Sheep BAP
*organism
Haemophilus