Microbio

Question 1

seven benches:

Answer 1

→ Media preparation
→ Streaking and Inoculation
→ Urine, Stool, and Respiratory
→ Exudates and CSF
→ Blood
→ Mycology and anaerobes
→ TB

Question 2

Manual media prep that utilizes a media dispenser that dispenses a specific volume (25 mL);

for sensitivity!

Answer 2

Mueller-Hinton Agar

Question 3

Which is not a Differential/Selective media

SSA
XLD
HEA
TM

Answer 3

TM! it is ENRICHED media for NEISSERIA

XLD for enteric;
SSA and HEA for Salmonella and Shigella;

Question 4

For fungi, this is the culture media

Answer 4

Sabouraud Dextrose Agar

Question 5

CAP is enriched media for these two fastidious organisms:

Answer 5

Haemophilus spp &

Neisseria spp

Question 6

In ___
145 µL of the sample in the test tube is transferred and
diluted to another test tube containing 3mL NSS

Answer 6

Vitek Compact which measures IgG and sensitivity

Question 7

Bactec FX principle;

Answer 7

Presence of bacteria = change in the CO2/O2 ratio = change in
the fluorescence of the blood specimen (in solid layer at bottom of bottle)

Question 8

Amt of blood needed to transfer to bottle

Answer 8

1-3 mL (infants)

→ 8 mL (adults)

Question 9

Carbolfuchsin stain for TB

showing Red bacilli against blue background

Answer 9

Kinyoun method

Question 10

Fluorescence Microscope

→ Fluorescence bacilli against black background

Answer 10

Acid- Fast Bacilli (AFB) smear;

AURAMINE stain

Question 11

Mycobacteria Growth Indicator Tube (MGIT) how many weeks to grwo

Answer 11

6 weeks

Question 12

Gram staining process

Answer 12

→ Crystal Violet (primary stain)
→ Gram’s Iodine (mordant)
→ 95% Ethyl Alcohol (decolorizer)
→ Safranin (counterstain/ secondary stain)

Question 13

India ink Used to identify for ;

Negative staining bc capsule to large to stain

Answer 13

Cryptococcus neoformans