Microbial Control Flashcards
All seven physical types of microbial control
- Heat
- Freezing/fridge
- Desiccation
- Filtration
- HEPA (High Efficiency Particulate Air)
- Osmotic
- Radiation
Types of heat related methods
- moist heat
- dry heat
Types of moist heat related methods
- boiling
- autoclaving (121 degrees C, 15 psi, 15 min)
- pasteurization
Pasteurization types
Batch: 30 min, 63 degrees
Flash: 15s, 72 degrees
Ultra-High temp pasteurization: 1s, 134 degrees
Ultra-high temp sterilization: 1s, 140 degrees
Radiation types
Ionizing—wavelength shorter than 1nm
—ejects e- forming OH- ions to destroy molecules (nucleic acids)
•electron beams-effectively kill, but take a while to penetrate
•gamma rays-effective at penetrating, take a while to kill
Non-ionizing—wavelength greater than 1nm
—excites e-, causing new covalent bond formation, affecting structure of proteins and nucleic acids
•UV light doesn’t penetrate well—useful for penetrating air, transparent fluids, surfaces
•260nm is most germicidal wavelength
Types of dry heat-related methods
- Hot air—2h at 160 degrees C or 1h at 170 C
- incineration—1s at more than 1000 C
All 10 chemical methods of microbial control
- Phenol/phenolics
- Alcohols
- Halogens
- Oxidizing Agents
- Surfactants
- Heavy Metals
- Aldehydes
- Gaseous Agents
- Enzymes
- Antimicrobials
Phenol/Phenolics
- Denatures Proteins and disrupts cell membranes
- Carbolic Acid, Triclosan, Lysol, Hexachlorophene
Alcohols
- Denatures proteins and disrupt cell membranes
- Isopropanol, Ethanol
Halogens
- Presumably denatures proteins
- iodine, bromine, chlorine (bleach, sodium hypochlorite, calcium, hypochlorite), fluorine
Oxidizing Agents
- denatures proteins through oxidization
- Peroxide’s, ozone, peracetic acid
Surfactants (Surface Active)
- breaks down surface tension and disrupts cell membrane
- Soaps, Detergents, Quats ((Quaternary Ammonium Compounds) Benzalkonium Chloride, Cetylpyridinium Chloride)
Heavy Metals
- Dentures proteins
- Arsenic, Zinc, Mercury, Silver, Copper, dental amalgam, silver nitrate
Aldehydes
- Denatures proteins
- glutaraldehyde, formaldehyde, formalin
Gaseous Agents
- Denatures proteins
- Ethylene Oxide, Propylene Oxide, beta-propiolactone