Microbial Control Flashcards

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1
Q

All seven physical types of microbial control

A
  • Heat
  • Freezing/fridge
  • Desiccation
  • Filtration
  • HEPA (High Efficiency Particulate Air)
  • Osmotic
  • Radiation
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2
Q

Types of heat related methods

A
  • moist heat

- dry heat

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3
Q

Types of moist heat related methods

A
  • boiling
  • autoclaving (121 degrees C, 15 psi, 15 min)
  • pasteurization
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4
Q

Pasteurization types

A

Batch: 30 min, 63 degrees
Flash: 15s, 72 degrees
Ultra-High temp pasteurization: 1s, 134 degrees
Ultra-high temp sterilization: 1s, 140 degrees

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5
Q

Radiation types

A

Ionizing—wavelength shorter than 1nm
—ejects e- forming OH- ions to destroy molecules (nucleic acids)
•electron beams-effectively kill, but take a while to penetrate
•gamma rays-effective at penetrating, take a while to kill
Non-ionizing—wavelength greater than 1nm
—excites e-, causing new covalent bond formation, affecting structure of proteins and nucleic acids
•UV light doesn’t penetrate well—useful for penetrating air, transparent fluids, surfaces
•260nm is most germicidal wavelength

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6
Q

Types of dry heat-related methods

A
  • Hot air—2h at 160 degrees C or 1h at 170 C

- incineration—1s at more than 1000 C

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7
Q

All 10 chemical methods of microbial control

A
  • Phenol/phenolics
  • Alcohols
  • Halogens
  • Oxidizing Agents
  • Surfactants
  • Heavy Metals
  • Aldehydes
  • Gaseous Agents
  • Enzymes
  • Antimicrobials
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8
Q

Phenol/Phenolics

A
  • Denatures Proteins and disrupts cell membranes

- Carbolic Acid, Triclosan, Lysol, Hexachlorophene

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9
Q

Alcohols

A
  • Denatures proteins and disrupt cell membranes

- Isopropanol, Ethanol

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10
Q

Halogens

A
  • Presumably denatures proteins

- iodine, bromine, chlorine (bleach, sodium hypochlorite, calcium, hypochlorite), fluorine

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11
Q

Oxidizing Agents

A
  • denatures proteins through oxidization

- Peroxide’s, ozone, peracetic acid

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12
Q

Surfactants (Surface Active)

A
  • breaks down surface tension and disrupts cell membrane

- Soaps, Detergents, Quats ((Quaternary Ammonium Compounds) Benzalkonium Chloride, Cetylpyridinium Chloride)

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13
Q

Heavy Metals

A
  • Dentures proteins

- Arsenic, Zinc, Mercury, Silver, Copper, dental amalgam, silver nitrate

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14
Q

Aldehydes

A
  • Denatures proteins

- glutaraldehyde, formaldehyde, formalin

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15
Q

Gaseous Agents

A
  • Denatures proteins

- Ethylene Oxide, Propylene Oxide, beta-propiolactone

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16
Q

Enzymes

A
  • Denature proteins

- lysozyme