Microarrays Flashcards
1
Q
What probes are synthesised?
A
All those for known and predicted mRNAs.!
2
Q
Why must you sequence the genome of other organisms?
A
Evolution studies
Vectors of diseases
Commercial
Model organisms
3
Q
What do you see if you run a gel of total RNA?
A
Smear of mRNA (thousands of different sizes)
Two bright bands (28 s and 18 s) of rRNA
4
Q
What is a gene chip?
A
Thin water of silicon carrying 1000s of nt probes
Oligonucleotides are synthesised directly onto surface of chip.
Hybridisation is electronically detected.
