Micro Quiz Flashcards

0
Q

Most pathogens are (ph)

A

Neutrophils

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1
Q

Most pathogens and microbes are (temp)

A

Mesophiles

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2
Q

When the ph changes

A

Enzymes are denatured

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3
Q

Human blood ph

A

7.4

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4
Q

Buffers

A

Used to retard ph changes

Example is strong acid buffer will weaken the acid

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5
Q

Zwitterions

A

Amino acids in media that are used as natural buffers

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6
Q

3 oxygen labs

A

Fluid thioglycollate
Anaerobic jar
Brain heart agar

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7
Q

Thioglycollate

A

-Used to grow aerobic and anaerobic microbes.
-Chemical sodium thioglycollate reduces oxygen to water.
-chemical resazurin acts as an indicator.
• pink is oxidized
• colorless is reduced

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8
Q

Anaerobic jar

A

-Creates anaerobic, microaerophilic, and capnophile conditions.
Contains gas pack and palladium

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9
Q

Gas pack in anaerobic jar does:

A

Produces hydrogen and carbons dioxide CO2

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10
Q

Palladium in anaerobic jar:

A

Combines hydrogen and oxygen O2

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11
Q

Brain and heart agar:

A

A molten agar that can be used the same way as thioglycollate.

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12
Q

Aerotolerance

A

Te ability or inability to live in the presence of oxygen

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13
Q

Anaerobic

A

Live at the bottom of a test tube

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14
Q

Aerobic

A

Live at the top of a tube

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15
Q

Obligate (strict) aerobe

A

Require oxygen so grow at the top

16
Q

Facultative anaerobes

A

Grow in presence or absence of oxygen. Grow throughout medium but appear denser near the top.

17
Q

Aerotolerant anaerobes

A

Do not require oxygen and are not. Adversely affected by it. Live uniformly throughout the medium.

18
Q

Microaerophiles

A

Survive only in environments containing lower than atmospheric levels of oxygen. Are seen in the middle or upper middle region of the medium

19
Q

Capnophiles

A

Survive only if carbon dioxide levels are elevated.

20
Q

Obligate ( strict) anaerobes

A

Even small amounts of oxygen are lethal. Will only be seen in the lower regions of the medium.

21
Q

Psychrophiles

Middle optimum maximum

A

Grow below 20 Celsius
-5 minimum
10 optimal
17 maximum

22
Q

Psychrotrophs

A

Grow from 0 to 30
Minimum is 0
Optimum is 22
Maximum 35

23
Q

Mesophiles

A

Between 15 and 45
Minimum is 15
Optimum is 35
Maximum is 45

24
Q

Thermophiles

A

Above 40
Minimum is 40
Optimum is 65
Maximum Is 78

25
Q

Thermophiles

A

Between 65 and 110
Minimum is 65
Optimum is 95
Maximum is 110

26
Q

Acidophiles

A

Grow well in below 5.5 ph

27
Q

Neutrophils

A

Grow well between 5.5 and 8.5

28
Q

Alkaliphiles

A

Grow well above 8.5