Micro Quiz Flashcards
Most pathogens are (ph)
Neutrophils
Most pathogens and microbes are (temp)
Mesophiles
When the ph changes
Enzymes are denatured
Human blood ph
7.4
Buffers
Used to retard ph changes
Example is strong acid buffer will weaken the acid
Zwitterions
Amino acids in media that are used as natural buffers
3 oxygen labs
Fluid thioglycollate
Anaerobic jar
Brain heart agar
Thioglycollate
-Used to grow aerobic and anaerobic microbes.
-Chemical sodium thioglycollate reduces oxygen to water.
-chemical resazurin acts as an indicator.
• pink is oxidized
• colorless is reduced
Anaerobic jar
-Creates anaerobic, microaerophilic, and capnophile conditions.
Contains gas pack and palladium
Gas pack in anaerobic jar does:
Produces hydrogen and carbons dioxide CO2
Palladium in anaerobic jar:
Combines hydrogen and oxygen O2
Brain and heart agar:
A molten agar that can be used the same way as thioglycollate.
Aerotolerance
Te ability or inability to live in the presence of oxygen
Anaerobic
Live at the bottom of a test tube
Aerobic
Live at the top of a tube