Mice Flashcards
What is the Genus name of the mouse?
Mus
What is the Species name of the mouse?
Mus musculus
What Order is the mouse from?
Rodentia
What Sub-Order is the mouse from?
Myomorpha
What Family is the mouse from?
Muridae
What Sub-Family is the mouse from?
Murinae
Mice account for what % of procedures involving genetically modified animals?
98%
What are the reasons mice are used in labs?
inexpensive small size short life span (2-3 years) can study many generations in short time period rapid reproduction (19-21 days) large litter size
What is the life span of mice?
2-3 years
What is the gestation period of mice?
19-21 days
What are the uses of mice in research?
genetics breeding cancer drug testing cosmetics virology histocompatability (tissue transfer) congenital defects aging radiobiology diabetes behavior
What is the average weight of an adult mouse?
20-40g
What is the birth weight/condition of mice?
1-5g
blind
naked
What is the weaning age of mice?
21-28 days
What is the normal temperature for mice?
97.5-100.4 F
What is the normal heart rate of mice?
325-780 bpm
What is the normal respiration rate of mice?
60-220/min
What is the pH of mouse urine?
7.3-8.5
What is the USG of mouse urine?
1.08
When is puberty for mice?
6-8 weeks
How long is the estrous cycle for mice?
4-5 days
T or F: Mice are polyestrus because of spontaneous ovulation
True
What is the gestation time of mice?
19-21 days
What is the litter size of the mouse?
1-20
What is the average litter size of mice?
6-12
When do mice pups first open their eyes?
12-13 days
How long does the vaginal plug persist in mice?
18-24 hours
How long is the breeding duration in mice?
7-9 months
When is breeding onset for female mice?
50-60 days
When is breeding onset for male mice?
50 days
The laboratory strains of mice used today are descendants of what?
european house mouse (Mus domesticus) with some genes from asian species
What did the mice breeders of the 19th century Europe develop and how did that impact later research?
developed the albino and color mutants and began the investigations of varietal characteristics and inheritance that provided stocks for later research
What is the Albino mouse also known as?
swiss mouse
What do mice use to feel around and why?
external vibrissae
they have poor eyesight
What is the name of the gland behind the eye of the mouse and what does it produce?
Harderian gland
produces red tears (porphorin, not blood)
What is the 1st digit of the mouse forepaw?
pollex
How is sex determined in mice?
ano-genital distance
What is the dental formula of mice?
2 (I 1/1, M 3/3) = 16
What type of incisors do mice have?
hypsodont
What type of molars do mice have?
brachyodont
How many mammary glands do mice have?
5
3 thoracic
2 abdominal
What type of fermentators are mice?
hind gut fermentators
T or F: Rodents have sweat glands and are able to pant
false
What is the acceptable environment temperature range for mice?
64-79 F
What should the environmental humidity range be maintained at?
30-70%
average: 50%
How many hours of light/day do mice need?
12-14
How many hours of light/day do mice need for breeding?
14 hours of light
How far should the bottom shelf of a rack be off the floor?
6 in. off the floor
What is the most common type of mouse cage?
plastic box
12 X 6 X 6 inches
metal lid
How much floor space is required for each mouse in a cage?
8 sq in per adult mouse
What is the ideal construction of a mouse cage?
seamless for easy
designed for easy cleaning
confine mice without restricting movement
stackable without binding
light weight yet sturdy
resistant to corrosive effects of mouse urine
What should the diameter of the water tube be for mice?
1/4 - 3/8 inch
What angle must the water tube be placed at?
bent at an angle of 105 degrees
Where is the tip of the water bottle placed in a mouse cage?
2 in. above the floor of the cage
What are the 3 purposes bedding material have?
provide material from which mice can make nests
absorb fecal and urine wastes
provide insulation
What are the most common bedding materials for mice?
wood shavings ground up corn cobs chopped straw hay shredded paper paper strands
How deep should bedding be in mice cages?
3/4 in. deep
How often should cages be cleaned?
atleast once a week
What form should mouse food be in?
pellets
What are factors that influence genetic background on breeding performance?
behavior
hybrid vigor
birth defects
What are mutation/transgenic associated breeding problems of mice ?
infertility (either gender) mammary function embryonic lethality abnormal behavior shortened breeding life span
What are the non-genetic factors that influence breeding performance of mice?
environment (temp, light intensity and light cycle, noise and vibrations, air pressure, odors)
handling consistency
nutrition
health status
Data collection and record keeping
collect breeding statistics
monitor genotype and gender frequencies for each breeding unit
pedigree records
What are the non productive breeder criteria of mice?
no litter produced (40 days from date of first mating)
no new litters (40 days from last date born)
no weaned pups (2-3 litters with no wean)
What are tips for colony management of mice?
mate mice early ( between 7-12 weeks of age)
establish/collect breeding stats
replace breeders on rotation (weekly, monthly)
keep good records, evaluate data regularly
remove non-productive breeders ASAP
What is the ideal way to set up a breeding cage?
1 male to 2-6 females
T or F: Pregnant female mice usually get along
true
When should mice pups be separated from their mother?
1 month old
When can tail clipping be done on mice?
after 18 days of age
What is the proper way to pick up a mouse?
grab at base of tail
allow forefeet to grasp edge of cage or table
approach from behind and place forefinger and index finger on either side of neck
hold rest of animal against palm with remaining fingers
What is the proper scruffing technique of a mouse?
hold at tail base
use forefinger and thumb to gently pin down the head
using the same fingers, grab a sufficent amount of loose skin over the neck
hold the tail with the little finger of the same hand for additional support
How much blood can you collect from the tail of a mouse?
0.1ml
What tools are needed for blood collection from the tail of a mouse?
75% alcohol cotton ball for disinfection small plastic bottle with 1/2 cm holes in both ends as mouse restrainer scissors pipette vial for blood collection
How much blood can be collected from the orbital sinus of mice?
up to 0.5ml
What tools are needed for blood collection from orbital sinus of mice?
75% alcohol ball for disinfection
hypnorm for general anesthetic
27 G needle with 1ml syringe for injection
glass capillary tube and vial for blood collection
How much blood can be collected from cardiac puncture in mice?
up to 1ml
What tools are needed for cardiac punch in mice?
75% alcohol cotton ball for disinfection
hypnorm used for anesthetic
27 G needle with 1ml syringe for injection
24 G needle with 3ml syring for blood withdrawl
What tools are needed for a saphenous blood draw in mice?
75% alcohol cotton ball 50 ml syringe tube with holes at each end scalpel and shaver to remove hair 24 G 1in. needle for release of blood tips and pipette for blood collection
How much blood can be collected from the saphenous vein in mice?
100 microliters
What tools are need for an IP injection in mice?
75% alcohol cotton ball
25 G 1/2 in. needle with 1ml syringe for injection
What is the maximum amount of fluids that can be given to a mouse at one dose IP?
1-2 ml
What tools are needed of SQ injection in mice?
75% alcohol cotton ball
25 G 1in. needle with 1ml syringe
What should the oral feeding in mice be limited to?
amount limited to 1% of body weight
What tools are needed for oral feeding of mice?
18 G stainless steel, ball tipped needle
glove
