Brainscape's Knowledge GenomeTM

Browse over 1 million classes created by top students, professors, publishers, and experts.


See full index

Mic Genetics > MIC428R Final > Flashcards

MIC428R Final Flashcards

You may prefer our related Brainscape-certified flashcards:
Biology 101 flashcards
1
Q

If two different restriction enzymes target the same sequence, the overhands will be able to bind to each other

A

False

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
2
Q

Which of the following istes on the bacterial chromosome can recombine with attP?
xis
attP
int
attB

A

attB

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
3
Q

Spacer arrays found in CRISPR loci are transcribed and then processed into small crRNA that target incoming DNA or RNA

A

True

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
4
Q

H-NS protein binds ti regions that are relatively high in GC%

A

False

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
5
Q

Which of the ofllowing proteins is an antiterminator?
M
P
N
O

A

N

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
6
Q

Phage lambda chooses the lysogenic cycle when CI repressor is not degraded by proteases

A

False

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
7
Q

Which of the following proteins is a site specific recombinase?
RecA
Int
TnpA
RecF

A

Int

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
8
Q

Phage lambda chooses the lytic cycle a majority of the time under normal conditions

A

true

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
9
Q

The Presence of what kind of motif specifies whether CRISPR complexes target incoming DNA?
Proto Spacer Adjacent (PAM) motif
CASCADE motif
Csy4 motif
Phage motif

A

PAM

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
10
Q

The restriction enzyme EcoR1 leaves what type of overhang?
4 base 3’
4 base 5’
a blund end

A

4 base 5’

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
11
Q

Once phage lambda is a lysogen, which of the following modifications will cause the phage to be permanently stuck as a lysogen?
Deletion of LexA
Modification of CI so that it cannot autocleave
deletion of CI
Modification of recA so that it cannot be activated

A

Modificaton of CI so that it cannot autocleave
Modification of recA so that it cannot be activated

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
12
Q

Which of the following could be recognized by a CRISPR spacer with the TEMPLATE sequence (note this doesn’t say crRNA, note the type of DNA/RNA targeted, and all the answers are the same length as the spacer, so don’t worry about length being different and assume the CRISPR can target ssDNA).

3’ AAAAAAAAAAAAAAAA 5’

ssDNA with a sequence 5’ AAAAAAAAAAAAAAAA 3’
ssDNA with a sequence 5’ GGGGGGGGGGGGGGGG 3’
ssDNA with a sequence 5’ AAAAAAAAAAAAAAAT 3’
ssDNA with a sequence 5’ TTTTTTTTTTTTTTTT 3’

A

ssDNA with a sequence 5’ AAAAAAAAAAAAAAAA 3’

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
13
Q

You select for a strain of E. coli that is RESISTANT to lysis/lysogeny from phage lambda. The mutation providing resistance eliminates the lambda receptor. The resistant strain will be able to grow well on which types of media?
M9 minimal media
M9 minimal media + glucose
LB
M9 minimal media + maltose

A

M9 minimal media + glucose
LB
M9 minimal media + maltose

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
14
Q

The restriction enzyme EcoRV leaves what type of overhand?
blunt end
4 base 3’
4 base 5’

A

blunt end

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
15
Q

The restriction enzyme Psti leaves what type of overhang?
4 base 5’
4 base 3’
blunt end

A

4 base 3’

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
16
Q

The order of the lac operon is lacZYA
If a Tn5 transposon lands in lacY, the phenotypic outcome would be:
White colonies on M9 mm+ lactose
Blue colonies on M9 mm + lactose
No colonies on M9 mm + lactose

A

no colonies on m9 mm + lactose

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
17
Q

The order of the lac operon is lacZYA
If a Tn5 transposon lands in lacZ, the phenotypic outcome would be:
White colonies on M9 mm + lactose
Blue colonies on M9 mm + lactose
No colonies on M9 mm + lactose

A

no colonies on m9 mm + lac

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
18
Q

The order of the lac operon is lacZYA
If a Tn5 transposon lands in lacZ, the phenotypic outcome would be:
White colonies on M9 mm + glucose + Xgal
Blue colonies on M9 mm + glucose + Xgal
No colonies on M9 mm + glucose + Xgal

A

white colonies on m0 mm + glucose + Xgal

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
19
Q

The order of the lac operon is lacZYA
If a Tn5 transposon lands in lacY, the phenotypic outcome would be:
White colonies on M9 mm + glucose + Xgal
Blue colonies on M9 mm + glucose + Xgal
No colonies on M9 mm + glucose + Xgal

A

Blue colonies on m9 mm + glucose + Xgal

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
20
Q

Mutation rates would likely decrease after UV exposure with deletion of which of the following genes?
recA
DNA photolyase
mutS
uvrA

A

recA

21
Q

Which of the following likelty interacts with RecA in any state?
SetR
DinI
C1
UmuC

A

All of them

22
Q

Which of the following DNA repair pathways does not require ligase?
Base excision repair
Nucleotide excision repair
Dimer separation by DNA photolyase
Methyl directed mismatch repair

A

Dimer separation by DNA photolyase

23
Q

Which of the following are phenotypes associated with deletion of recA?
Decreased mutation rate when exposed to UV radiation
Decreased site specific recombination of ICE elements
Decreased homologous recombination rates
Increased sensitivity to UV radiation
Increased induction of Lambda prophage
Decreased induction of Lambda prophage

A

Decreased mutation rate when exposed to UV
Decreased homologous recombination rates
Increased sensitivity to UV
Deceased induction of lambda phage

24
Q

RecFOR changes its function when it encouters chi sites

A

False

25
Q

Hihly expressed genes will experience lower overall mutation rates than lowly expressed genes

A

True

26
Q

Deletion of DAM methylasewill lead to increased mutation rates within E. coli

A

true

27
Q

Deletion of DNA Pol IV will lead to increased mutation rates within E. coli cells

A

False

28
Q

ICE elements (like SXT) experience high levels of conjugation in cells where SetR has been genetically modified so that it cannot be cleaved

A

False

29
Q

Transcription coupled repair recruits UvrAB to help carry out nucleotide excision repair in damaged regions of DNA

A

True

30
Q

E coli will be less sensitive to damage by UV radiation if they are grown in light

A

True

31
Q

RecA changes its function when it encounters chi sites

A

False

32
Q

RecBCD changes its function when it encounters chi sites

A

True

33
Q

E coli cells will be more sensitive to damage by ROS if they are grown in light

A

False

34
Q

Vibrio cells produce small peptides (translated from ORFs) that act as quorum sensing signals

A

False

35
Q

Under high osmolarity conditions, EnvZ will act as a
Phosphatase
Kinase

A

Kinase

36
Q

OmpR can carry out the following functions
Phosphorylation of OmpR
Binding to OmpC promoter
Binding to OmpF promoter
De-phosphorylation of OmpR

A

Bind to OmpC and OmpF promoters

37
Q

Gram positive cells produce small peptides that act as quorum sensing signals

A

True

38
Q

Bacterial cells from different species can communicate with one another through quorum sensing

A

True

39
Q

Which of the following will complement a Luxl mutant in Vibrio?
Cell free supernatant form a:
LuxR- strain
LuxR+ strain
Luxl- strain
Luxl+ strain
None of the above

A

LuxR+ and Luxl+ strains

40
Q

EnvZ can carry out the following functions
Phosphorylation of OmpR
Binding to OmpC promoter
De-phosphorylation of OmpR
Binding to OmpF promoter

A

Phosphorylation of OmpR
De-phosphorylation of OmpR

41
Q

Each bacterial species makes only one types of quorum sensing signal

A

false

42
Q

Phosphorylated NtrB binds more strongly to DNA than unphosphorylated NtrB

A

false

43
Q

OmpF provides a relatively large channel in the inner membrane

A

false

44
Q

Which of the following will likely increase expression of trpB in E. coli
Deletion of the polyU region downstream the leader peptide in the attenuator region
Deletion of TrpA
Nonsense mutation in TrpA
Deletion of anti-TRAP

A

Deletion of the polyU region downstream the leader peptide in the attenuator region

45
Q

Which of the following is directly regulated by changes in the ratio of uncharged/charged tryptophan tRNA
trpE expression
Attenuator region leader peptide expression
TRAP binding to RNA
TrpR binding to DNA

A

trpE expression

46
Q

Which of the following is constitutively expressed
RpoS
RpoH
RpoD
RpoN

A

RpoH
RpoD
RpoN

47
Q

Which of the following will likely decrease expression of the trpB in E. coli
A nonsense mutation in the second codon of the leader peptide
Deletion of the attenuator region
Deletion of TRAP
A nonsense mutation in trpE

A

A nonsense mutation in the second codon of the leader peptide

48
Q

Which of the following is directly involved in positive regulation of the lac operon?
Lac operator
LacZ
LacI
CAP binding site

A

CAP binding site

49
Q
A

Mic Genetics (2 decks)

Brainscape helps you reach your goals faster, through stronger study habits.
© 2025 Bold Learning Solutions. Terms and Conditions