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Microscope > Mic > Flashcards

Mic Flashcards

1
Q

What is the total magnification of an object using a 6x Ocular and 30x objective lens?

A

TM= Ocular x Objective Lens

TM= 6x . 30x = 180x

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2
Q

Resolution:

A

The ability of Lens to separate or distinguish between small Objects that are close together

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3
Q

Parfocal

A
  • A microscope that can remain in focus when the objective lens is changed.
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4
Q

Ocular

A

10x Magnification

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5
Q

Revolving Nosepiece

A

Rotates between objectives

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6
Q

Objective

A

Different modes of power lenses from low to High

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7
Q

Stage

A

Place where slide supports it and is placed

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8
Q

Mechanical Stage Knob

A

Moves stage from left to right and from back and forth

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9
Q

Condenser/Condenser knob

A

Focuses light on speciment

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10
Q

Iris Diaphragm

A

Controls the amount that passes

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11
Q

Light Source

Light intensity Knob

A

Projects Light Up

Controls the intensity

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12
Q

Coarse Focus

A

Big Movement of the stage up and down

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13
Q

What is the Purpose of a coverslip

A

1) it protects the microscope’s objective lens from contacting the specimen, and (2) it creates an even thickness (in wet mounts) for viewing.

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14
Q

What is the Total Magnification when you use Low Power Lens of Your Microscope

A

TM = 10x . 10x = 100x

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15
Q

What 2 parts of the microscope affect the amount of light passing through the specimen

A

Iris Diaphgram & Light intensity

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16
Q

when increasing lens magnification why do you need to increase the amount of light on the specimen?

A

When you change from low power to high power on a microscope, the high-power objective lens moves directly over the specimen, and the low-power objective lens rotates away from the specimen

17
Q

Always start with

A

Lowpower 10x

18
Q

Why should one not use coarse focus when using the 40x lens?

A

you run the risk of crashing the objective into the slide.

19
Q

Which requires more light: a stained specimen slide or an unstained live specimen slide? why?

A

stained specimen

20
Q

Immursion oil

A
  • Increase resolution of a microscope.

- the oil has the same optical qualities as glass and prevents refractive loss and increases the numerical aperture.

Microscope (1 decks)

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