Mic Flashcards
What is the total magnification of an object using a 6x Ocular and 30x objective lens?
TM= Ocular x Objective Lens
TM= 6x . 30x = 180x
Resolution:
The ability of Lens to separate or distinguish between small Objects that are close together
Parfocal
- A microscope that can remain in focus when the objective lens is changed.
Ocular
10x Magnification
Revolving Nosepiece
Rotates between objectives
Objective
Different modes of power lenses from low to High
Stage
Place where slide supports it and is placed
Mechanical Stage Knob
Moves stage from left to right and from back and forth
Condenser/Condenser knob
Focuses light on speciment
Iris Diaphragm
Controls the amount that passes
Light Source
Light intensity Knob
Projects Light Up
Controls the intensity
Coarse Focus
Big Movement of the stage up and down
What is the Purpose of a coverslip
1) it protects the microscope’s objective lens from contacting the specimen, and (2) it creates an even thickness (in wet mounts) for viewing.
What is the Total Magnification when you use Low Power Lens of Your Microscope
TM = 10x . 10x = 100x
What 2 parts of the microscope affect the amount of light passing through the specimen
Iris Diaphgram & Light intensity
when increasing lens magnification why do you need to increase the amount of light on the specimen?
When you change from low power to high power on a microscope, the high-power objective lens moves directly over the specimen, and the low-power objective lens rotates away from the specimen
Always start with
Lowpower 10x
Why should one not use coarse focus when using the 40x lens?
you run the risk of crashing the objective into the slide.
Which requires more light: a stained specimen slide or an unstained live specimen slide? why?
stained specimen
Immursion oil
- Increase resolution of a microscope.
- the oil has the same optical qualities as glass and prevents refractive loss and increases the numerical aperture.
