MHC

Question 0

How many domains does the class one have

Answer 0

3 alpha domains, with a non MHC molecule stabilizer, B2 microglobulin

Question 1

Group of class one molecules ensuring tolerance of the mother to the baby

Answer 1

G antigen

Question 2

MHC class 1

Answer 2

Closed
Accommodates 12- 10 amino acid chains
Ubiquitous
Bulging peptides
Cd8 cells
Uses IL 2, gamma IFN, GSSF, Tumor necrosis factor and lymphotoxin
Cellular immune response
One transmembrane attachment
2-3 pockets

Question 3

MHC class 2

Answer 3

Open ends, more accommodating
Accommodates13-18 AA chains
Double transmembrane attachment
Double chains
Present only on apcs, immune competent cells
CD4 cells
Humoral immune response
Uses IL4, 5, 6, 10
Flat peptides

Question 4

Molecular weights of heavy and light chains of mhc 1

Answer 4

HEAVY CHAIN- 45,000

Light chain- 12,000

Question 5

Molecular weights of heavy and light chains of mhc 2

Answer 5

Alpha chain;heavy chain- 33,000

Beta chain; light chain- 27,000

Question 6

Chromosome coding b2 microglobulin

Answer 6

Chromosome 15

Question 7

Chromosome coding for MHC

Answer 7

Chromosome #6

Question 8

Mhc equivalent in mice and where is it located?

Answer 8

H-2 ; chromosome 17

Question 9

Gene domain for class 1

Answer 9

HLA- A
HLA- B
HLA- C

Minor hlA molecules, EFG

Question 10

Gene domain for class 2

Answer 10

HLA- D ONLY

Specifically DQ, DR, DP

Question 11

Most highly polymorphic B gene

Answer 11

DR B - 18 known allelic polymorphisms

Question 12

Allelic polymorphisms for class 1 genes

Answer 12

HLA -A; 530 alleles
HLA -B; 921 alleles
HLA -C; 312 alleles

Question 13

MHC genes are considered; and inherited as a package

Answer 13

Codominant; haplotype with both chromosome pairs 6 having a set of alleles

Question 14

Class 3 MHC codes for

Answer 14

Complement proteins (c4 and 2) and cytokines, factor b

Question 15

Ranking for transplantation and immune response
Class one
Class two
Class three

Answer 15

Immune response: c2,c1,c3

Transplantation: c1, c2, c3

Question 16

Similar for HLA and H-2

Answer 16

Class 3

Question 17

Minor histocompatibility complex chromosome

Answer 17

23

Question 18

Class 1 is found ubiquitously except in

Answer 18

RBCs and spermcells

Question 19

Binding sites for mhc class 1

Answer 19

A2 and A1

Question 20

Binding sites for mhc class 2

Answer 20

A1 and B2

Question 21

Class one mhc site components and process

Answer 21

Made in the ER.
Calnexin holds the 3 domain alpha chain into a partially folded structure to allow binding of B2 microglobulin
The binding process happens in the ER
The proteins are degraded first by proteosomes, cleaved and then DELIVERED BY TAP1 and TAP2
Tapasin brings TAP into close proximity of mhc molecules and mediates interaction with them
It is then transferred at the cell membrane.

Question 22

Class one mhc site components and process

Answer 22

Also made in the ER
Binding site is is within the phagocytic vesicles( endocytic)
Most potent activators for T cells are dendritic cells
The invariant chain, a 31kd molecule prevents interaction with endogenous peptides
Once bound to an invariant chain, it is transported in to a endosomal compartment(vesicle) and then waits for processed exogenous peptides
Once it encounters a peptide, the invariant chain is degraded into a CLIP(class 2 invariant chain peptide) and is exchanged for exogenous peptides (low ph favors this binding)

Question 23

Peptide bonding present within class 2 mhc

Answer 23

Hydrogen bonds

Question 24

Molecular ruler for peptide binding of MHC 1

Answer 24

Transporter associated with antigen processing

Question 25

Type of peptide bonding between peptide and class one mhc

Answer 25

Bond happens in amino and carboxy terminal ends