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Lab > Methods of Blood Collection > Flashcards

Methods of Blood Collection Flashcards

1
Q

Skin puncture is the choice of blood collection for which patients?

A

Pediatric, obese, severe burns or thrombotic tendencies

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2
Q

What site is skin puncture collected from?

A

Heel, finger, earlobe or toe

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3
Q

Which fingers should be used for skin puncture?

A

Middle or ring finger
Middle is the first choice

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4
Q

What is the best method of blood collection?

A

Venipuncture

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5
Q

How should lancet be placed in correlation to the striations of the finger tip?

A

Across the striations

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6
Q

What is the max depth for venipuncture?

A

2mm

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7
Q

How many degrees should the capillary tube be placed?

A

Approximately 15 degrees

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8
Q

What angle should the needle be during venipuncture?

A

15 degrees

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9
Q

Where should the bevel be for venipuncture?

A

Bevel up

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10
Q

What anticoagulant is in a purple top?

A

Ethylenediaminetetracetic Acid (EDTA)

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11
Q

What studies does a purple top perform?

A

CBC w/ and w/o diff
ESR
A1C

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12
Q

What is the most widely used anticoagulant?

A

Ethylenediaminetetracetic Acid (EDTA)

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13
Q

How does EDTA work?

A

Binds calcium so blood cannot clot

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14
Q

How long can purple top specimens be refrigerated?

A

24 hours

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15
Q

What anticoagulant is in blue tops?

A

Sodium citrate

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16
Q

What studies does blue top perform?

A

Coagulation studies
- Prothrombin time (PT)
- Partial Thromboplastin Time (PTT)
- Fibrinogen (Fib)
- D-Dimer

17
Q

How does sodium citrate work?

A

Binds to calcium

18
Q

How long are blue tops good for?

A

4 hours after collection

19
Q

What anticoagulant is in green tops?

A

Heparin

20
Q

What studies does a green top perform?

A

Plasma and chemistry studies
- Ammonia
- Adrenocorticotropic studies

21
Q

How does heparin work?

A

Inhibits clotting enzyme thrombin by creating antithrombin

22
Q

What anticoagulant does red top use?

A

None

23
Q

What studies are red tops for?

A

Chemistry, serum

24
Q

What does tiger top contain?

A

Serum Separator Gel

25
Q

What are grey tops used for?

A

Glucose studies, ETOH

26
Q

What do grey tops inhibit?

A

Glycolosis

27
Q

Should you draw blood from IV sites?

A

No, due to dilution of sample

28
Q

What happens in prolonged TQ application?

A

Blood cell concentration (Hemoconcentration)

29
Q

What kind of tubes should you first draw blood into?

A

Non additive tubes and then tubes with additives

30
Q

Order of tubes?

A

Blood culture tubes, blue, red, tiger/yellow (SST), green, lavender, grey

31
Q

What happens if you short fill a tube?

A

Improper coagulant/blood ratio

32
Q

Should you draw blood while a patient is standing?

A

Nah

33
Q

What could cause infection during blood draw?

A

Inadequate cleaning

Lab (4 decks)

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