Methods of Blood Collection Flashcards

1
Q

What is the method of choice for taking pediatric patients blood? What else is it useful for?

A

Capillary Puncture. Useful with obesity, severe burns, and thrombotic tendencies.

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2
Q

where can you take a capillary puncture from?

A

heel, finger, earlobe, or toe

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3
Q

which part of the finger would you take a capillary puncture?

A

fleshy surface of distal phalanx of middle finger or ring finger. ({middle is preferred)

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4
Q

What is the most common site for capillary puncture in infants?

A

plantar surface of the heel, perpendicular to the big toe

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5
Q

What is the “best method of blood collection”?

A

venipuncture

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6
Q

specimens from venous blood, because of their ease, are a primary source of specimens for which purposes?

A

clinical laboratory analyses

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7
Q

Why can you use either 70% ETOH or Betadine Solution? One vs the other?

A

patient sensitivities/allergies

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8
Q

how many times do you milk or massage the middle or ring finger on a fingerstick capillary method of blood collection?

A

5-6 times

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9
Q

What can happen if you don’t allow the alcohol to dry on the patients skin prior to lancing?

A

it can cause the blood to hemolyze

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10
Q

where is your stab with a fingerstick?

A

side of the finger (off center) across the striations of the fingertip

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11
Q

the max depth of the lancet should not exceed how far into the fingertip?

A

2 MM

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12
Q

Why would you wipe away the first drop of blood on a fingerstick?

A

to avoid dilution of the specimen with tissue fluid

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13
Q

how should you position the patient with venipuncture procedures?

A

lying in bed or sitting in a chair with arm propped up
(never perform with a patient standing up)
use caution to ensure patient does not fall forward from chair

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14
Q

how far above the antecubital area should you place a tourniquet for a venipuncture?

A

2-3 inches

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15
Q

if you use a blood pressure cuff on a patient who has hard veins to find (probably super fat), what pressure in mm/Hg can you inflate the cuff?

A

30 mm/Hg

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16
Q

what is the angle for insertion of the needle in venipuncture?

A

15-30 degree angle with skin, bevel up

17
Q

how long should patient hold pressure after removing the needle in venipuncture?

A

3 min

18
Q

What anticoagulant is used in PURPLE tops?

A

Ethylenediaminetetracetic Acid (EDTA)

19
Q

What are PURPLE tops typically used for?

A
hematological studies: 
CBC
WBC diff
ESR 
AIC
20
Q

how does EDTA work?

A

binds calcium so blood cannot clot

21
Q

Does EDTA affect cellular morphology?

A

no

22
Q

How long can specimens be refrigerated with PURPLE tops?

A

24 hrs

23
Q

What anticoagulant is used in LIGHT BLUE tops?

A

Sodium Citrate

24
Q

Sodium Citrate/Light Blue Tops are typically used in which studies?

A
coagulation studies:
PT
PTT
Fib
D Dimer
25
Q

How does Sodium Citrate work?

A

a chelating agent that binds calcium.

26
Q

How long are LIGHT BLUE tops good for after collection?

A

Typically only 4 hours

27
Q

What anticoagulant is in the GREEN tops?

A

Heparin

28
Q

GREEN tops are used in which types of studies?

A

Plasma Studies and Chem Studies:

Ammonia 
Adrenocorticotropic hormones (ACTH)
29
Q

How does Heparin work as an anticoagulant?

A

inhibits the clotting enzyme, thrombin, by creating an antithrombin. (prevents conversion of prothrombin into thrombin)

30
Q

What are RED tubes for? Is there any agent in them?

A

No anticoagulant or additives. Used for Chem, Serology, tests requiring SERUM.

31
Q

What are Serum Separator Tubes (SST)/Gold/Tiger Top/HIV Tube (red/yellow) used for?

A

serum separator gel primarily used for chem testing. No anticoagulant.

32
Q

What is in the GREY top tube?

A

Sodium Fluoride.

33
Q

What is the GREY top tube used for?

A

glucose studies
ETOH level analysis

inhibits glycolysis

34
Q

What happens with prolonged application of a tourniquet during phlebotomies?

A

increase in blood cell concentration (also called hemoconcentration)

35
Q

What can happen if you don’t follow the proper “order of draw”

A

possible contamination

36
Q

This is the word for what happens when the integrity of the vein is compromised and blood is leaking into the tissue?

A

Hematoma