methods Flashcards

1
Q

why did you use yeast as a model system

A
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2
Q

disadvantages of yeast as a model system

A
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3
Q

Why SEY6210?

A
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4
Q

imaging - why used?

A
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5
Q

endogenous tagging - method?

A
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6
Q

transformation - method?

A
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7
Q

plasmids - method?

A
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8
Q

plasmids - advantages?

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9
Q

plasmids - disadvantages?

A
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10
Q

BIFC - technique?

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11
Q

BIFC - alternatives - why not?

A
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12
Q

FRAP- technique?

A
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13
Q

protein purification - technique?

A
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14
Q

NBD-labelling - technique/science?

A
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15
Q

PI4P transfer assay - technique?

A
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16
Q

why stopped using PI4P transfer assays?

A
17
Q

PM integrity - technique/science?

A
18
Q

GCAMP better than dye

A
19
Q

HPLC - technique?

A
20
Q

Mass spectrometry - technique?

A
21
Q

lipotype vs nozumo?

A
22
Q

cryo-ET

A
23
Q

chapter 3 -summarise

A
24
Q

chapter 3 - one sentence

A
25
Q

chapter 3 - key result

A
26
Q

chapter 3 - novelty

A
27
Q

chapter 3 -previous data that inspired this work?

A